Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Experimental Transfer of Tetracycline Resistance Genes from Fish-derived Bacteria to Escherichia coli

  • Kim Seok-Ryel (Division of Food Science and Biotechnology, Pukyong National University) ;
  • Kim Hyeung-Rak (Division of Food Science and Biotechnology, Pukyong National University) ;
  • Suzuki Satoru (Center for Marine Environmental Studies (CMES), Ehime University)
  • Published : 2006.06.01
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Abstract

To determine whether the tetracycline resistance genes tet (34), tet (M), and tet (S) can be transferred among bacteria, we used a filter mating experiment allowing intimate cell-cell contact between donor and recipient. The tet(34) gene, conveyed on a chromosome of Vibrio species (No. 6 and SW-42) was not transferred to Escherichia coli JM109, suggesting that it is not transferred among bacterial species. The tet (M) gene was transferred from three Vibrio strains (4-E, SW-18, and SW-38) to E. coli at frequencies of $8.5{\times}10^{-5}\;to\;2.1{\times}10^{-6}$. The tet(S) gene was transferred from Lactococcus garvieae KHS98032 to E. coli at a frequency of $1.8{\times}10^{-6}$. Transconjugated recipients showed increased minimum inhibitory concentrations against oxytetracycline. Although the donors possess the Tn916-Tn1545 transposons, they were not detected in transformed recipients, suggesting that the transfer of tet(M) and tet(S) is mediated by elements or mechanisms. Two ribosomal protect protein genes were also transmissible from marine bacteria to E. coli, suggesting gene hopping among marine, terrestrial, and human environments.

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References

  1. Aoki, T., T. Arai, and S. Egusa. 1977. Detection of R plasmids in naturally occurring fish-pathogenic bacteria, Edwardsiella tarda. Microbiol. Immunol., 21, 77-83 https://doi.org/10.1111/j.1348-0421.1977.tb02810.x
  2. Aoki, T., T. Satoh and T. Kitao. 1987. New tetracycline resistance determinant of R plasmid from Vibrio anguillarum. Antimicrob. Agents Chemother., 31, 1446-1449 https://doi.org/10.1128/AAC.31.9.1446
  3. Chandrasekaran, S., B. Venkatesh and D. Lalithakumari. 1998. Transfer and expression of a multiple antibiotic resistance plasmid in marine bacteria. Curr. Microbiol. 37, 347-351 https://doi.org/10.1007/s002849900390
  4. Charpentier, E. and P. Courvalin. 1999. Antibiotic resistance in Listeria spp. Antimicrob. Agents Chemother., 43, 2103-2108
  5. Charpentier, E., G. Gerbaud and P. Courvalin. 1994. Presence of the Listeria tetracycline resistance gene tet(S) in Enterococcus faecalis. Antimicrob. Agents Chemother., 38, 2330-2335 https://doi.org/10.1128/AAC.38.10.2330
  6. Chopra, I. and M. Roberts. 2001. Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiol. Mol. Biol. Rev., 65, 232-260 https://doi.org/10.1128/MMBR.65.2.232-260.2001
  7. Clewell, D.B., S.E. Flannagan and D.D. Jaworski. 1995. Unconstrained bacterial promiscuity: the Tn916-Tn1545 family of conjugative transposons. Trends Microbiol., 3, 229-236 https://doi.org/10.1016/S0966-842X(00)88930-1
  8. De Barbeyrac, B., M. Dupon, P. Rodriguez, H. Renaudin and C. Bebear. 1996. A Tn1545-like transposon carries the tet(M) gene in tetracycline resistant strains of Bacteroides ureolyticus as well as Ureaplasma urealyticum but not Neisseria gonorrhoeae. J. Antimicrob. Chemother., 37, 223-232 https://doi.org/10.1093/jac/37.2.223
  9. DePaola, A., P.A. Flynn, R.M. McPherarson and S.B. Levy. 1988. Phenotypic and genotypic characterization of tetracycline and oxytetracycline-resistant Aeromonas hydrophila from cultured channel catfish (Ictalurns punctatus) and their environments. Appl. Environ. Microbiol., 54, 1861-1863
  10. Francois, B., M. Charles and P. Courvalin. 1997. Conjugative transfer of tet(S) between strains of Enterococcus faecalis is associated with the exchange of large fragments of chromosomal DNA. Microbiology, 143, 2145-2154 https://doi.org/10.1099/00221287-143-7-2145
  11. Ho, S.P., T.Y. Hsu, M.H. Chen and W.S. Wang. 2000. Antibacterial effect of chloramphenicol, thiamphenicol and florfenicol against aquatic animal bacteria. J. Vet. Med. Sci., 62, 479-485 https://doi.org/10.1292/jvms.62.479
  12. Kim, S.R., L. Nonaka, M.J. Oh, C.R. Lavilla-Pitogo and S. Suzuki. 2003. Distribution of an oxytetracycline resistance determinant tet(34) among marine bacteria isolates of a Vibrio species. Microbes Environ., 18, 74-81 https://doi.org/10.1264/jsme2.18.74
  13. Kim, S.R., L. Nonaka and S. Suzuki. 2004. Occurrence of tetracycline resistance genes tet(M) and tet(S) in bacteria from marine aquaculture sites. FEMS Microbiol. Lett., 237, 147-156 https://doi.org/10.1111/j.1574-6968.2004.tb09690.x
  14. Levy, S.B. 1988. Tetracycline resistance determinants are widespread. ASM News, 54, 418-421
  15. Miranda, C.D., C. Kehrenberg, C. Ulep, S. Schwarz and M.C. Roberts. 2003. Diversity of tetracycline resistance genes in bacteria from Chilean salmon farms. Antimicrob. Agents Chemother., 47, 883-888 https://doi.org/10.1128/AAC.47.3.883-888.2003
  16. Nonaka, L. and S. Suzuki. 2002. New Mg$^{2+}$-dependent oxytetracycline resistance determinant Tet 34 in Vibrio isolates from marine fish intestinal contents. Antimicrob. Agents Chemother., 46, 1550-1552 https://doi.org/10.1128/AAC.46.5.1550-1552.2002
  17. Perreten, V., F. Schwarz, L. Cresta, M. Boeglin, G. Dasen and M. Teuber. 1997. Antibiotic resistance spread in food. Nature, 389, 801-802
  18. Rhodes, G., G. Huys, J. Swings, P. McGann, M. Hiney, P. Smith and R.W. Pickup. 2000. Distribution of oxytetracycline resistance plasmids between aeromonads in hospital and aquaculture environments: implication of Tn1721 in dissemination of the tetracycline resistance determinant Tet A. Appl. Environ. Microbiol., 66, 3883-3890 https://doi.org/10.1128/AEM.66.9.3883-3890.2000
  19. Rice, L.B. 1998. Tn916 family conjugative transposons and dissemination of antimicrobial resistance determinants. Antimicrob. Agents Chemother., 42, 1871-1877
  20. Roberts, M.C. 1996. Tetracycline resistance determinants: mechanisms of action, regulation of expression, genetic mobility, and distribution. FEMS Microbiol. Rev., 19, 1-24 https://doi.org/10.1111/j.1574-6976.1996.tb00251.x
  21. Salyers, A.A., N.B. Shoemaker, A.M. Stevens, and L.Y. Li. 1995. Conjugative transposons: an unusual and diverse set of integrated gene transfer elements. Microbiol. Rev., 59, 579-590
  22. Sandaa, R.A., V. Torsvik and J. Goksoyr. 1992. Transferable drug resistance in bacteria isolated from fish farm sediments. Can. Microbiol., 42, 1061-1065