Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effect of In Vitro Maturation Time of Porcine Immature Oocytes on the Subsequent Development and Hatching after In Vitro Fertilization

돼지 미성숙 난자의 체외 성숙 시간이 배 발생과 부화에 미치는 영향

  • Kim J.Y. (Infertility Medical Center of CHA General Hospital) ;
  • Park H. (Infertility Medical Center of CHA General Hospital) ;
  • Kim J.M. (College of Medicine, Pochon CHA University) ;
  • Lee J.H. (College of Medicine, Pochon CHA University) ;
  • Park Y.S. (Kyongbuk Livestock Research Institute) ;
  • Kwak D.S. (Department of Biotechnology, Daegu University) ;
  • Park H.D. (Department of Biotechnology, Daegu University)
  • 김재영 (차병원 여성의학연구소 불임의학연구소) ;
  • 박향 (차병원 여성의학연구소 불임의학연구소) ;
  • 김재명 (포천중문의과대학교) ;
  • 이정형 (포천중문의과대학교) ;
  • 박용수 (경상북도 축산기술연구소) ;
  • 곽동석 (대구대학교 공과대학 생명공학과) ;
  • 박흠대 (대구대학교 공과대학 생명공학과)
  • Published : 2006.06.01
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Abstract

This study was conducted to investigate the effect of in vitro maturation (IVM) duration of porcine follicular oocytes on maturation rate, polyspermic rate, and subsequent embryo development. The nuclear maturation rates of oocytes matured for 36, 38, 40, 42 and 44 hr were similar between 68.0, 78.0, 79.5, 73.8 and 81.8% respectively. There was no significant difference in the rates of polyspermy after in vitro feritilization (IVF). The cleavage rate in the group of 36 hr was significantly higher in than that of 40, 44 hr (p<0.05) but not to 38 and 42 hr. The development rate to blastocyst stage was significantly higher in the group of 38 hr (23.1%) than that in the group of 44 hr (15.6%) (p<0.05) but not to 36, 40 and 42 hr. These results suggest that the aged oocytes for 44 hr is not required for the production of bias to cysts derived from porcine IVF embryos.

본 연구는 돼지 체외 수정란의 생산에 있어서 체외성숙 시간이 핵성숙, 다정자 침입율 및 배 발생과 배반포의 부화율 배반포의 부화에 미치는 효과를 검토하였다. 돼지 난포란의 핵성숙율이 체외성숙 36, 38, 40, 42 및 44 시간째에 각각 68.0, 78.0, 79.5, 73.8 및 81.8%로서 각 군간에 유사한 경향이었으며, 체외 수정 후 다정자 침입율도 각각 48.7, 36.0, 44.4, 38.9 및 31.8%로서 차이가 없었다. 체외 성숙 시간에 따른 2세포기 발달율은 체외 성숙 36시간군이 77.3%로서 가장 높았고, 체외 성숙 40 및 44 시간의 70.6 및 70.3%와는 유의차가 인정되었다(p<0.05). 배반포 발달율은 38시간군의 23.1%가 체외 성숙 44 시간군의 15.6%보다 유의하게 높았다(p<0.05). 따라서 돼지 난포란의 44시간 체외 성숙이 배 발생에 비효과적이었다.

Keywords

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