Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
권호 표지

Effects of Culture Conditions on The Expression of Cyclin B1 Protein during the First Meiotic Maturation in Bovine Immature Oocytes

  • Published : 2006.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Cyclin B1 is known to reflect the M-phase promoting factor (MPF), a universal regulator of G2/M-phase transition, activity during the process of oocytes maturation. To investigate whether culture condition affects the maturation rate and the expression of cyclin B1 protein, bovine immature oocytes are stimulated and cultured according to the following protocols: Experiment 1: denuded oocytes (denude) only, COC only, denuded oocytes+granulosa cells (denude+GCs) and COC+GCs; Experiment 2: no-activation (control), 7% ethanol for 5 min and $10{\mu}l/ml$ ionomycin for 5 min at immediately before maturation. The maturation rates of denude and no-activation group were significantly lower in both experiments (P<0.05), respectively. Co-culture or stimulation method in bovine immature oocytes culture increases the cyclin B1 expression significantly in both experiments (P<0.05). Based on these results, culture condition affects the maturation rate and the expression of cyclin B1 protein during the first meiotic maturation in bovine immature oocytes.

Keywords

References

  1. Bogliolo L, Ledda S, Leoni G, Naitana S, Moor RM (2000): Activity of maturation promoting factor (MPF) and mitogen-activated protein kinase (MAPK) after parthenogenetic activation of ovine oocytes. Cloning 2:185-196 https://doi.org/10.1089/152045500454744
  2. Buccione R, Schoeder AC, Eppig JJ (1990): Interactions between somatic cells and germ cells throughout mammalian oogenesis. Biol Reprod 43:543-547 https://doi.org/10.1095/biolreprod43.4.543
  3. Choi T, Aoki F, Mori M, Yamashita M, Magahama Y, Kohmoto K (1991): Activation of p34cdc2 protein kinase activity in meiotic and mitotic cell cycles in mouse oocytes and embryos. Development 113:789-795
  4. Dirnfeld M, Goldman S, Yael G (1997): A simplified coculture system with luteinzed granulosa cells improves embryo quality and implantation rates: A controlled study. Fertil Steril 67:120-122 https://doi.org/10.1016/S0015-0282(97)81867-5
  5. Evans T, Rosenthal ET, Youngblom J, Distel D, Hunt T (1983): Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division. Cell 33:389-396 https://doi.org/10.1016/0092-8674(83)90420-8
  6. Fukui Y (1990): Effects of follicle cells on the acrosome reaction, fertilization and developmental competence of bovine oocytes matured in vitro. Mol Reprod Dev 26:40-46 https://doi.org/10.1002/mrd.1080260107
  7. Hample A, Eppig JJ (1995): Analysis of the mechanism(s) of metaphase I arrest in maturing mouse oocytes. Development 121:925-933
  8. Hashimoto N, Kishimoto T (1988): Regulation of meiotic metaphase by a cytoplasmic maturation-promoting factor during mouse oocytes maturation. Dev Biol 126:242-252 https://doi.org/10.1016/0012-1606(88)90135-2
  9. Janssenswillen C, Nagy ZP, Van Steirteghem A (1995): Maturation of human cumulus-free germinal vesicle-stage oocytes to metaphase II by coculture with monolayer vero cells. Hum Reprod 10:375-378 https://doi.org/10.1093/oxfordjournals.humrep.a135947
  10. Kim CW, Chung KH (2004): MAP kinase is activated during the maturation of porcine oocytes, Asian-Aust J Anim Sci 17:1069-1075 https://doi.org/10.5713/ajas.2004.1069
  11. Levesque JT, Sirard MA (1996): Resumption of meiosis is initiated by the accumulation of cyclin B in bovine oocytes. Biol Reprod 55:1427-1436 https://doi.org/10.1095/biolreprod55.6.1427
  12. Marangos P, Carroll J (2004): The dynamics of cyclin Bl distribution during meiosis I in mouse oocytes. Reproduction 128:153-162 https://doi.org/10.1530/rep.1.00192
  13. Masui Y (1974): A cytostatic factor in amphibian oocytes: its extraction and partial characterization. J Exp Zool 187:141-147 https://doi.org/10.1002/jez.1401870116
  14. Mattioli M, Galeati G, Seren E (1988): Effects of follicle somatic cells during pig oocytes maturation on egg penetrability and male pronucleus formation. Gamete Res 20:177-183 https://doi.org/10.1002/mrd.1120200208
  15. Minamihashi A, Watson AJ, Watson PH, Church RB, Schultz GA (1993): Bovine parthenogenetic blastocysts following in vitro maturation and oocyte activation with ethanol. Theriogenology 4:63-76
  16. Park YS (2004): Effects of IVM and IVF duration on in vitro development and cell numbers of embryos in Korean native cattle. Reprod Dev Biol 28:221-226
  17. Rabahi F, Monniaux D, Pisselet C, Chupin D, Durand P (1991): Qualitative and quantitative changes in protein synthesis of bovine follicular cells during the preovulatory period. Mol Reprod Dev 30:265-274 https://doi.org/10.1002/mrd.1080300315
  18. Winston N (1997): Stability of cyclin B protein during meiotic maturation and the first meiotic cell cycle division in mouse oocytes. Biol Cell 89:211-219 https://doi.org/10.1016/S0248-4900(97)80037-8
  19. Zhang L, Jiang S, Wozniak PJ, Yang X, Godke RA (1995): Cumulus cell function during bovine oocyte maturation, fertilization, and embryo development in vitro. Mol Reprod Dev 40:338-344 https://doi.org/10.1002/mrd.1080400310