Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Growth Regulation in IGF-1 Receptor Transgenic Mice

  • Kim Hyun-Joo (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Shin Young-Min (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Chang Suk-Min (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Park Chang-Sik (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Jin Dong-Il (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University)
  • Published : 2006.06.01
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Abstract

To study the signaling effect of insulin-like growth factor-I(IGF-1), transgenic mice containing IGF-1 Receptor (IGF-1R) cDNA fused to metallothionein promoter were produced by DNA microinjection into the pronucleus of mouse zygote. Three founders were produced with transgenic mice containing IGF-1R gene. Transgenic mice lines contained approximately $4{\sim}20$ copies of transgenes per cell and transmission of this gene into the progeny with Mendelian manner were determined. The founder mice were mated with normal mice to produce $F_1$ mice and then $F_2$ mice. Transmission rates of IGF-1R transgene in the progeny mice were $25{\sim}60%$ in $F_1$ generation and $40{\sim}50%$ in $F_2$ generation. The mRNA expression of IGF-1R transgene in liver was analyzed using RT-PCR for IGF-1R gene in liver. When body weights of transgenic pups were measured during 4, 10 and 14 weeks after birth, IGF-1R transgenic mice grew faster than non transgenic littermates. This study indicated that growth regulation by IGF-1 signaling through IGF-1R can be elucidated using IGF-1R transgenic mice.

Keywords

References

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