Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지

Effect of Mitomycin-C on Rabbit Conjunctival Fibroblast Proliferation and Apoptosis

마이토마이신이 가토 결막섬유아세포의 증식 및 사멸에 미치는 효과

  • Kwon Young-Sam (School of Veterinary Medicine, University of Wisconsin-Madison) ;
  • Jang Kwang-Ho (College of Veterinary Medicine, Kyungpook National University)
  • 권영삼 (위스콘신-매디슨 대학교 수의과대학) ;
  • 장광호 (경북대학교 수의과대학)
  • Published : 2006.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to determine the effects of mitomycin C on conjunctival fibroblast proliferation and apoptosis. Rabbit conjunctival fibroblast mono layers were treated with 48 hours application of mitomycin-C. The viability of cells was evauated by MTT assay. To examine the effect of mitomycin-C on the cell proliferation, immunocytochemistry for BrdU staning was performed. Then, we investigated whether mitomycin-C induced cell's apoptosis by TUNEL staining. As a result of MTT assay, the viability of cells was gradually inhibited in a dose dependent manner by mitomycin-C. The BrdU staining showed that mitomycin-C significantly suppressed the proliferation of conjunctival fibroblast at concentrations of 0.02% or more. When TUNEL assays were performed, the number of apoptotic cells increased 5.6-, 18.5-, and 33.8-fold compared with the control at 0.01, 0.02, and 0.04%, respectively, of mitomycin-C at 48 hours of exposure. Therefore, mitomycin-C may be useful as a regulator in the treatment of corneal diseases that manifest with scar formation and tumor of fibroblast expansion.

Keywords

References

  1. Bergstrom TJ, Wilkinson WS, Skuta GL, Watnick RL, Elner VM. The effects of subconjunctival mitomycin-C on glaucoma filtration surgery in rabbits. Arch Ophthalmol 1991; 109: 1725-1730 https://doi.org/10.1001/archopht.1991.01080120109038
  2. Crooke ST, Bradner WT. Mitomycin C: a review. Cancer Treat Rev 1976; 3: 121-139 https://doi.org/10.1016/S0305-7372(76)80019-9
  3. Crowston JG, Wang XY, Khaw PT, Zoellner H, Healey PR. Human serum reduces mitomycin-C cytotoxicity in human tenon's fibroblasts. Invest Ophthalmol Vis Sci 2006; 47: 946-952 https://doi.org/10.1167/iovs.05-1048
  4. Frucht-Pery J, Rozenman Y. Mitomycin C therapy for corneal intraepithelial neoplasia. Am J Ophthalmol 1994; 117: 164-168 https://doi.org/10.1016/S0002-9394(14)73072-7
  5. Glover TL, Nasisse MP, Davidson MG. Effects of topically applied mitomycin-C on intraocular pressure, facility of outflow, and fibrosis after glaucoma filtration surgery in clinically normal dogs. Am J Vet Res 1995; 56: 936-940
  6. Jampel HD. Effect of brief exposure to mitomycin C on viability and proliferation of cultured human Tenon's capsule fibroblasts. Ophthalmology 1992; 99: 1471-1476 https://doi.org/10.1016/S0161-6420(92)31781-6
  7. Khaw PT, Sherwood MB, MacKay SL, Rossi MJ, Schultz G. Five-minute treatmerits with fluorouracil, floxuridine, and mitomycin have long-term effects on human Tenon's capsule fibroblasts. Arch Ophthalmol 1992; 110: 1150-1154 https://doi.org/10.1001/archopht.1992.01080200130040
  8. Kim TI, Tchah H, Lee SA, Sung K, Cho BJ, Kook MS. Apoptosis in keratocytes caused by mitomycin C. Invest Ophthalmol Vis Sci 2003; 44: 1912-1917 https://doi.org/10.1167/iovs.02-0977
  9. Mamede FV, Laus JL, Cabral VP, Vicenti FA, Barbieri-Neto J. Use of topical mitomycin C in myoplasty of the medial rectus muscle of rabbits. Vet Ophthalmol 2004; 7: 335-342 https://doi.org/10.1111/j.1463-5224.2004.04026.x
  10. Schipper I, Suppelt C, Gebbers JO. Mitomycin C reduces scar formation after excimer laser (193nm) photorefractive keratectomy in rabbits. Eye 1997; 11: 649-655 https://doi.org/10.1038/eye.1997.171
  11. Singh G, Wilson MR, Foster CS. Mitomycin eye drops as treatment for pterygium. Ophthalmology 1988; 95: 813-821 https://doi.org/10.1016/S0161-6420(88)33104-0
  12. Smith S, D'Amore PA, Dreyer EB. Comparative toxicity of mitomycin C and 5-fluorouracil in vitro. Am J Ophthalmol 1994; 118: 332-337 https://doi.org/10.1016/S0002-9394(14)72957-5
  13. Talamo JH, Gollamudi S, Green WR, De La Cruz Z, Filatov V, Stark WJ. Modulation of corneal wound healing after excimer laser keratomileusis using topical mitomycin C and steroids, Arch Ophthalmol 1991; 109: 1141-1146 https://doi.org/10.1001/archopht.1991.01080080101040
  14. Wilson MW, Hungerford JL, George SM, Madreperla SA. Topical mitomycin C for the treatment of conjunctival and corneal epithelial dysplasia and neoplasia, Am J Ophthalmol 1997; 124: 303-311 https://doi.org/10.1016/S0002-9394(14)70822-0
  15. Xu H, Liu S, Xia X, Huang P, Wang P, Wu X. Mitomycin C reduces haze formation in rabbits after excimer laser photorefractive keratectomy. J Refract Surg 2001; 17: 342-349
  16. Yamamoto T, Varani J, Soong HK, Lichter PR. Effects of 5-fluorouracil and mitomycin C on cultured rabbit subconjunctival fibroblasts. Ophthalmology 1990; 97:1204-1210 https://doi.org/10.1016/S0161-6420(90)32456-9