한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제23권2호
  • /
  • Pages.144-148
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  • 2006
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

개 연골세포의 손상에 의한 Tartrate Resistant Acid Phosphatase 활성의 변화 측정

The Change of Tartrate Resistant Acid Phosphatase Activity in Capsaicin-Induced Canine Chondrocyte Death

  • 설재원 (헬스케어 사업단, 전북대학교 수의과대학 생체안전성 연구소) ;
  • 이해범 (헬스케어 사업단, 전북대학교 수의과대학 생체안전성 연구소) ;
  • 김남수 (헬스케어 사업단, 전북대학교 수의과대학 생체안전성 연구소) ;
  • 김인식 (헬스케어 사업단, 전북대학교 수의과대학 생체안전성 연구소) ;
  • 강형섭 (헬스케어 사업단, 전북대학교 수의과대학 생체안전성 연구소) ;
  • 이영훈 (전북대학교 치과대학 구강생체과학연구소) ;
  • 강동원 (송파동물병원) ;
  • 박상열 (헬스케어 사업단, 전북대학교 수의과대학 생체안전성 연구소)
  • Seol Jae-Won (Center for Healthcare Technology Development, Bio-safety Research Institute College of Veterinary Medicine Chonbuk National University) ;
  • Lee Hae-Beom (Center for Healthcare Technology Development, Bio-safety Research Institute College of Veterinary Medicine Chonbuk National University) ;
  • Kim Nam-Soo (Center for Healthcare Technology Development, Bio-safety Research Institute College of Veterinary Medicine Chonbuk National University) ;
  • Kim In-Shik (Center for Healthcare Technology Development, Bio-safety Research Institute College of Veterinary Medicine Chonbuk National University) ;
  • Kang Hyung-Sub (Center for Healthcare Technology Development, Bio-safety Research Institute College of Veterinary Medicine Chonbuk National University) ;
  • Lee Young-Hoon (Institute of Oral Bioscience, School of Dentistry Chonbuk National University) ;
  • Kang Dong-Won (Song Pa Animal Clinic) ;
  • Park Sang-Youel (Center for Healthcare Technology Development, Bio-safety Research Institute College of Veterinary Medicine Chonbuk National University)
  • 발행 : 2006.06.01
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초록

Apoptotic death of articular chondrocytes has been implicated in the pathogenesis of osteoarthritis. Tartrate resistant acid phosphatase (TRAP) has been used for several years as a marker enzyme of bone-resorbing osteoclasts. This study investigated the activity of TRAP in media of apoptotic cell death-induced canine chondrocyte. We exposed canine chondrocyte to capsaicin and the results showed that capsaicin induced cell death in a dose dependent manner. And we measured TRAP activity in media of chondrocyte death induced by capsaicin treatment and the results capsaicin significantly increased the activity of TRAP in media for dose dependent. We also investigated whether the combination treatment with capsaicin and TRAIL enhance apoptotic cell death in canine chondrocyte. We exposed canine chondrocyte to capsaicin for 24 hrs at the indicated dose, and then treated with recombinant TRAIL protein for 24 hrs. TRAIL alone did not induce cell death after 24 hours, but the combined treatment of both induced more cell death compared with capsaicin alone in a dose dependent manner. Also, the combination treatment with capsaicin and TRAIL increased the activity of TRAP in culture media. These results suggest that TRAP can flow out into extracellular after chondrocyte damage, and TRAP may be a successful biomarker for detection of joint disease such as osteoarthritis.

키워드

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