Variation of Cannabinoids Content in Hemp (Cannabis sativa L.) Produced with Mixed Seeds of Drug and Non-drug Type Varieties

마약형 및 저 마약형 품종 혼합파종에 의해 채종한 대마의 cannabinoids 함량 변이

  • 문윤호 (농촌진흥청 작물과학원 목포시험장) ;
  • 송연상 (농촌진흥청 작물과학원 목포시험장) ;
  • 정병춘 (농촌진흥청 작물과학원 목포시험장) ;
  • 방진기 (농촌진흥청 작물과학원 목포시험장)
  • Published : 2006.06.01

Abstract

In order to produce hemp with low cannabinoids and appropriate fiber yield, seeds of the IH3 (non drug type germplasm) and local variety (drug type cultivar) were mixed to 1:1, 2:1, 3:1 on base of seed weight ratio and cultivated for seed production. In the seed yield trial, the weight of 1000 grains were around 21 g, which was increased in proportion to the ratio of IH3 seeds but seed yield were 100 kg/10a, which were not significantly different by the seeds-mixing ratio. In fiber yield trials with seeds from seed yield trial, fiber yield ranged $193{\sim}198kg/10a$ which was not significantly different by the seeds mixing ratio but the content of THC(${\Delta}$-9-tetrahydrocannabinol) was lowered in proportion to the ratio of IH3 seeds. Variation of THC and CBD (Cannabidiol) level in the 150 plant cultivated with various seed mixing ratio reflected that the percentage of drug and intermediate type were decreased but non drug type were increased in proportion to the ratio of IH3 seeds.

저 마약형 대마 품종이 육성되지 않았을 경우 그 대안으로서 섬유용 대마의 THC 함량을 낮추기 위해 저 마약형 도입 유전자원중 경장은 작지만 개화기가 재래종과 비슷한 IH3 과 재래종 종자를 1:1, 2:1, 3:1로 혼합하여 채종시험을 실시하고 각 혼합비율별로 채종한 종자를 섬유용으로 재배하여 종실용 및 섬유용 대마 생육특성과 수량, 그리고 cannabinoid 함량분포를 검정한 결과를 요약하면 다음과 같다. 채종시험에서 IH3 의 비율이 클수록 천립중은 무거웠으나 종실수량은 100 kg/10a 내외로 차이가 없었다. 혼합비율별로 집단 채종한 종자를 섬유용으로 재배하였을 때 섬유수량은 $193{\sim}198kg/10a$로 비슷하였으나 THC 함량은 종자 IH3 혼합비율이 클수록 낮아져 3:1 에서는 0.39%이었다. THC 및 CBD 함량분포에서도 IH3 혼합비율이 높을수록 마약형 및 중간형 비율이 낮아졌고 저 마약형 비율은 증가하였다.

Keywords

References

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