Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Effect of Plant Growth Regulators and Medium Supplements on Plant Regeneration of Kentucky Bluegrass

식물생장조절물질과 배지첨가물질이 켄터키 블루그래스의 식물체 재분화에 미치는 영향

  • Lee Sang-Hoon (Division of Applied Life Science, College of Agriculture & Life Science, Gyeongsang National University) ;
  • Lee Ki-Won (Division of Applied Life Science, College of Agriculture & Life Science, Gyeongsang National University) ;
  • Kim Do-Hyun (Division of Applied Life Science, College of Agriculture & Life Science, Gyeongsang National University) ;
  • Lee Dong-Gi (Division of Applied Life Science, College of Agriculture & Life Science, Gyeongsang National University) ;
  • Won Sung-Hye (Division of Applied Life Science, College of Agriculture & Life Science, Gyeongsang National University) ;
  • Kim Ki-Yong ;
  • Lee Byung-Hyun (Division of Applied Life Science, College of Agriculture & Life Science, Gyeongsang National University)
  • 이상훈 (경상대학교 농업생명과학대학 응용생명과학부 낙농학) ;
  • 이기원 (경상대학교 농업생명과학대학 응용생명과학부 낙농학) ;
  • 김도현 (경상대학교 농업생명과학대학 응용생명과학부 낙농학) ;
  • 이동기 (경상대학교 농업생명과학대학 응용생명과학부 낙농학) ;
  • 원성혜 (경상대학교 농업생명과학대학 응용생명과학부 낙농학) ;
  • 김기용 (축산연구소 조사료자원과) ;
  • 이병현 (경상대학교 농업생명과학대학 응용생명과학부 낙농학)
  • Published : 2006.06.01
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Abstract

To optimize tissue culture responses for genetic transformation of Kentucky bluegrass, the effects of culture medium supplements on tissue culture responses were investigated with mature seeds of a cultivar 'Newport' as explant tissues. The optimal concentration of 2,4-D (2.4-dichloro phenoxy acetic acid) for the induction of embryogenic callus from mature seed was 3 mg/L. Plant regeneration frequency was 54% when embryogenic callus was cultured on the regeneration medium supplemented with 1 mg/L 2,4-D and 3 mg/L of BA (6-benzyladenine). Addition of 1 g/L of casein hydrolysate and 500 mg/L of L-proline improved frequencies of embryogenic callus induction and plant regeneration up to 60.8% and 58.3%, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study. We suggest that the results may be useful for molecular breeding of Kentucky bluegrass through genetic transformation.

켄터키 블루그래스의 최적 조직 배양조건을 확립하기 위하여 성숙종자로부터 배발생 캘러스 유도 및 식물체 재분화에 미치는 배지 첨가물질의 영향을 조사하였다. 배발생 캘러스 유도시 첨가되는 auxin으로는 3 mg/L 2,4-D 처리구에서 58.3%로 캘러스 유도율이 가장 높았으며, 3 mg/L 2,4-D와 0.1 mg/L의 BA를 첨가하여 배양했을 때 형성된 캘러스는 형태적으로도 우수하였고 조직적으로 치밀하며 유백색을 띤 배발생 캘러스가 가장 많이 형성되었으며 54%의 재분화율을 나타내었다. 성숙종자를 배양함에 있어 탄소원으로는 캘러스 유도배지 30 g/L sucrose, 재분화배지에 30 g/L maltose를 첨가해 주었을 때 효율이 증가되었다. 성숙종자를 배양함에 있어 첨가물질로 캘러스 유도배지와 재분화 배지에 1 g/L의 casein hydrolysate와 500 mg/L의 L-proline을 동시에 첨가해주었을 때 캘러스 유도율과 재분화율이 각각 60.8%와 58.3%로 증가되었다. 본 연구를 통하여 확립된 효율적인 배발생 캘러스의 유도 및 식물체 재분화 체계의 확립은 Agrobacterium을 이용한 켄터키 블루그래스의 형질전환에 효율적으로 이용될 수 있을 것으로 사료된다.

Keywords

References

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