Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Biodegradation of Hydrocarbon Contamination by Immobilized Bacterial Cells

  • Rahman Raja Noor Zaliha Abd. (Enzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Sciences) ;
  • Ghazali Farinazleen Mohamad (Enzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Sciences) ;
  • Salleh Abu Bakar (Enzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Sciences) ;
  • Basri Mahiran (Enzyme and Microbial Technology Research, Faculty of Science, Universiti Putra Malaysia)
  • Published : 2006.06.01
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Abstract

This study examined the capacity of immobilized bacteria to degrade petroleum hydrocarbons. A mixture of hydrocarbon-degrading bacterial strains was immobilized in alginate and incubated in crude oil-contaminated artificial seawater (ASW). Analysis of hydrocarbon residues following a 30-day incubation period demonstrated that the biodegradation capacity of the microorganisms was not compromised by the immobilization. Removal of n-alkanes was similar in immobilized cells and control cells. To test reusability, the immobilized bacteria were incubated for sequential increments of 30 days. No decline in biodegradation capacity of the immobilized consortium of bacterial cells was noted over its repeated use. We conclude that immobilized hydrocarbon-degrading bacteria represent a promising application in the bioremediation of hydrocarbon-contaminated areas.

Keywords

References

  1. Alexander, M. 1999. Biodegradation and Bioremediation, 2nd ed. Academic Press, San Diego
  2. Birnbaum, S. 1993. Immobilization of Macromolecules and Cells. p.23-35. In D.B. Sleytr, P. Messner, D. Pum, and M. Sara (eds) Immobilized Macromolecules: Application Potentials. Springer Verlag, Germany
  3. Bruheim, P., H. Bredholt, and K. Eimhjellen. 1997. Bacterial degradation of emulsified crude oil and the effects of various surfactants. Can. J. Microbiol. 43, 17-22 https://doi.org/10.1139/m97-003
  4. Chang, Y.C. and C.C. Chou. 2002. Growth and production of cholesterol oxidase by alginate-immobilized cells of Rhodococcus equi No. 23. Biotechnol. Appl. Biochem. 35, 69-74 https://doi.org/10.1042/BA20010058
  5. Chen, K.C., J.J. Chen, and J.Y Houng. 2000. Improvement of nitrogen removal efficiency using immobilized microorganisms with oxidation-reduction potential monitoring. J. Ind. Microbiol. Biotechnol. 25, 229-234 https://doi.org/10.1038/sj.jim.7000061
  6. Del'Arco, J.P., and F.P. Franca. 2001. Influence of contamination levels on hydrocarbon biodegradation in sandy sediment. Environ. Poll. 110, 515-519
  7. Diaz, M.P., K.G. Boyd, S.J.W. Grigson, and J.G. Burgess. 2002. Biodegradation of crude oil across a wide range of salinities by an extremely halotolerant bacterial consortium MPD-M, immobilized onto polypropylene fibers. Biotech. Bioeng, 79, 145-153 https://doi.org/10.1002/bit.10318
  8. Gardea-Torresdey, J.L., J.L. Arenas, N.M.C. Francisco, K.J. Tiemann, and R. Webb. 1998. Ability of immobilized cyanobacteria to remove metal ions from solution and demonstration of the presence of metallothionein genes in various strains. J. Hazardous Substance Research 1(2), 1-8
  9. Huy, N.Q., S. Jin., K. Amada, M. Haruki, N.B. Huu, D.T Hang, D.TC. Ha, T. Imanaka, M. Morikawa, and S. Kanaya. 1999. Characterization of petroleum-degrading bacteria from oil-contaminated sites in Vietnam. J. Biosci. Bioeng, 88, 100-102 https://doi.org/10.1016/S1389-1723(99)80184-4
  10. Manohar, S., C.K. Kim, and TB. Karegoudar. 2001. Enhanced degradation of naphthalene by immobilization of Pseudomonas sp. Strain NGKI in polyurethane foam. Appl. Microbiol. Biotechnol. 55, 311-316 https://doi.org/10.1007/s002530000488
  11. Manohar, S. and TB. Karegoudar. 1998. Degradation of naphthalene by cells of Pseudomonas sp. strain NGK1 immobilized in alginate, agar and polyacrylamide. Appl. Microbiol. Biotechnol. 49, 785-792 https://doi.org/10.1007/s002530051247
  12. Ohta, T., J.C. Ogbonna, H. Tanaka, and M. Yajima. 1994. Development of a fermentation method using immobilized cells nnder nnsterile conditions. 2. Ethanol and L-lactic acid production without heat and filter sterilization. Appl. Microbiol. Biotech. 42, 246-260 https://doi.org/10.1007/BF00902724
  13. Radwan, S.S., R.H. Al-Hasan, S. Salamah, and S. Al-Dabbous. 2002. Bioremediation of oily sea water by bacteria immobilized in biofilms coating microalgae. Int. Biodet. Biodeg, 50, 55-59 https://doi.org/10.1016/S0964-8305(02)00067-7
  14. Salleh, A.B., F. Mohamad Ghazali, R.N.Z. Abd Rahman, and M. Basri. 2003. Bioremediation of petroleum hydrocarbon pollution. Ind. J Biotechnol. 2, 411-425
  15. Tope, A.M., K. Jamil, and T.R. Baggi. 1999. Transformation of 2,4,6-trinitrotoluene (TNT) by immobilized and resting cells of Arthrobacter sp. J. Hazardous Substance Research. 2(3), 1-8
  16. Wang, C.C., C.M. Lee, C.J. Lu, M.S. Chuang, and C.Z. Huang. 2000. Biodegradation of 2,4,6-trichlorophenol in the presence of primary substrate by immobilized pure culture bacteria. Chemosphere. 41, 1873-1879 https://doi.org/10.1016/S0045-6535(00)00090-4