Journal of Microbiology and Biotechnology

  • 제16권6호
  • /
  • Pages.939-945
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  • 2006
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Function of Cell-Bound and Released Exopolysaccharides Produced by Lactobacillus rhamnosus ATCC 9595

  • Kim Ji-Uk (Division of Food Science, Korea University) ;
  • Kim Young-Hoon (Division of Food Science, Korea University) ;
  • Han Kyoung-Sik (Division of Food Science, Korea University) ;
  • Oh Se-Jong (Institute of Agricultural Science & Technology, Department of Animal Science, Chonnam National University) ;
  • Whang Kwang-Youn (Bioscience & Technology, Korea University) ;
  • Kim Jai-Neung (Department of Packaging, Yonsei University) ;
  • Kim Sae-Hun (Division of Food Science, Korea University)
  • 발행 : 2006.06.01
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초록

The physiological characteristics and function of the exopolysaccharide (EPS) produced by Lactobacillus rhamnosus ATCC 9595 were determined. The total quantity of EPS was rapidly increased to 496$\pm$20 mg/l during the exponential phase, and then maintained steadily during the stationary phase. During the exponential phase (18 h), the total EPS consisted of 61% cell-bound EPS (cb-EPS) and 39% released EPS (r-EPS), whereas the relative proportion of EPS during the stationary phase (48 h) was convered to 23% cb-EPS and 77% r-EPS. On gel permeation chromatography, cb-EPS was fractionated as a single peak of 8.6$\times10^6$ Da, whereas r-EPS was fractionated as two peaks with average molecular weights of 4.3$\times$10$^4$ and 8.6$\times10^6$ Da. Interestingly, both EPS species exhibited anticancer properties and cholera toxin-binding activities. Our results suggest that the EPS generated by L. rhamnosus ATCC 9595 might be suitable for use as a functional food or food supplement.

키워드

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