Journal of Microbiology and Biotechnology

  • 제16권6호
  • /
  • Pages.849-854
  • /
  • 2006
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Influence of Extracellular Products from Haematococcus pluvialis on Growth and Bacteriocin Production by Three Species of Lactobacillus

  • Kim Jeong-Dong (Institute of Industrial and Biotechnology, Department of Biological Engineering, Inha University) ;
  • Lee Choul-Gyun (Institute of Industrial and Biotechnology, Department of Biological Engineering, Inha University)
  • 발행 : 2006.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The effects of Haematococcus pluvialis extracellular products on microbial growth and bacteriocin production were investigated to improve bacteriocin synthesis during the growth cycle of Lactobacilli. Lactobacillus pentosus KJ-108, L. plantarum KJ-10311, and L. sakei KJ-2008 were cultured in MRS and enriched medium (ERM) with or without supplement of the extracellular products obtained from a late exponential phase culture of Haematococcus pluvialis in modified Bold's basal medium (MBBM). In both MRS and ERM, the extracellular products strongly enhanced the growth as well as the bacteriocin production of all the lactic acid bacteria tested. The enhancing effect was observed in ERM with pH adjusted at 5 and 6. In addition, some difference in growth effects with the extracellular products of H. pluvialis was observed between pH 5 and 6 in ERM, but no effect was observed in the minimal medium. The final biomass and the final concentration of bacteriocin activity were associated with the cell growth that was promoted by the extracellular products of H. pluvialis, and the enhanced cell growth of the three lactic acid bacterial strains induced the increase of the specific bacteriocin production. Therefore, bacteriocin production and activity were influenced by the addition of the extracellular products of H. pluvialis in the culture medium.

키워드

참고문헌

  1. Barefoot, S. F. and T. R. Klaenhammer. 1983. Detection and activity of lactacin B, a bacteriocin produced by Lactobacillus acidophilus. Appl. Environ. Microbiol. 45: 1808-1815
  2. Choi, H. J., M. J. Seo, J. C. Lee, C. I. Cheigh, H. Park, C. Ahn, and Y. R. Pyun. 2005. Heterologous expression of human ${\beta}$-defensin-1 bacteriocin-producing Lactobacillus lactis. J. Microbiol. Biotechnol. 15: 330-336
  3. Cuozzo, S. A., F. Sesma, J. M. Palacios, A. P. de Ruz Holgado, and R. R. Raya. 2000. Identification and nucleotide sequence of genes involved in the synthesis of lactocin 705, a two-peptide bacteriocin from Lactobacillus casei CRL 705. FEMS Microbiol. Lett. 185: 157-161 https://doi.org/10.1111/j.1574-6968.2000.tb09055.x
  4. De Vuyst, L. R. Callewaert and K. Crabbe. 1996. Primary metabolite kinetics of bacteriocin biosynthesis by Lactobacillus amylovorus and evidence for stimulation of bacteriocin under unfavorable growth conditions. Microbiology 142: 817-827 https://doi.org/10.1099/00221287-142-4-817
  5. Fooks, L. J. and G. R. Gibson. 2002. In vitro investigation of the effects of probiotics and prebiotics on selected human intestinal pathogens. FEMS Microbiol. Lett. 39: 67-75 https://doi.org/10.1111/j.1574-6941.2002.tb00907.x
  6. Gibson, G. R. and M. B. Roberfroid. 1995. Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics. J. Nutr. 125: 1401-1412
  7. Grasdelt, S. L., A. M. Bon, R. Bergers, M. Suschetet, and P. Astorg. 1998. Dietary carotenoids inhibit alflatoxin B2- induced liver preneoplastic foci and DNA damage in the rat; role of the modulation of alflatoxin Bi metabolism. Carcinogenesis 19: 403-411 https://doi.org/10.1093/carcin/19.3.403
  8. Grung, M., F. M. L. D'Souza, M. Borowitzka, and S. Liaaen-Jensen. 1992. Algal carotenoids 51, secondary carotenoids 2.Haematococcus pluvialis aplanospores as a source of (3S, 3'S)-astaxanthin esters. J. Appl. Phycol. 4: 165-171 https://doi.org/10.1007/BF02442465
  9. Harker, M., A. J. Tsavalos, and A. J. Young. 1996. Autotrophic growth and carotenoid production of Haematococcus pluvialis in a 30-1 air-lift photobioreactor. J. Ferment. Bioeng. 82: 113-118 https://doi.org/10.1016/0922-338X(96)85031-8
  10. Hume, R. D. and W. D. Hann. 1984. Growth of Legionella, Escherichia, and Pseudomonas among cells of the green algae Chlorella, Gloeocystis, and Scenedesmus as seen by scanning electron microscopy. Micron Microscop. Acta 15: 107-108 https://doi.org/10.1016/0739-6260(84)90013-3
  11. Kawai, Y., B. Saitoh, O. Takahashi, H. Kitazawa, T. Saito, H. Nakajima, and T. Itoh. 2000. Primary amino acid and DNA sequences of gassericin T: A lactacin F-family bacteriocin produced by Lactobacillus gasseri SBT2055. Biosci. Biotechnol. Biochem. 64: 2201-2208 https://doi.org/10.1271/bbb.64.2201
  12. Kim. H.-J., J.-H. Kim, J. H. Son, H.-J. Seo, S.-J. Park, N.-S. Park, and S.-K. Kim. 2004. Characterization of bacteriocin produced by Lactobacillus bulgaris. J. Microbiol. Biotechnol. 14: 503-508
  13. Kim, J.-D. and K.-H. Kang. 2004. Isolation of Lactobacillus sakei strain KJ-2008 and its removal of characteristic malodorous gases under anaerobic culture conditions. Biosci. Biotechnol. Biochem. 68: 2427-2435 https://doi.org/10.1271/bbb.68.2427
  14. Kim, J.-D., J.-H. Yoon, Y.-H. Park, D.-W. Lee, K.-S. Lee, C.-H. Choi, W.-Y. Park, and K.-H. Kang. 2003. Isolation and identification of a lactic acid bacterium strain KJ-108 and its capability for deodorizing malodorous gases under anaerobic culture conditions. J. Microbiol Biotechnol. 13: 207-216
  15. King, J. O. L. 1968. Lactobacillus acidophilus as a growth stimulant for pigs. The Veterinarian 5: 273-280
  16. Kulik, M. M. 1995. The potential for using cyanobacteria (blue-green algae) and algae in the biological control of plant pathogenic bacteria and fungi. Eur. J. Plant Pathol. 101: 585-599 https://doi.org/10.1007/BF01874863
  17. Lee, J. H., M. Kim, D. W. Jeong, M. Kim, J. H. Kim, H. C. Chang, D. K. Chung, H. Y. Kim, K. H. Kim, and H. J. Lee. 2005. Identification of bacteriocin-producing Lactobacillus paraplantarum first isolated from kimchi. J. Microbiol. Bacteriol. 15: 428-433
  18. Margalith, P. Z. 1999. Production of ketocarotenoids by microalgae. Appl. Microbiol. Biotechnol. 51: 431-438 https://doi.org/10.1007/s002530051413
  19. Mataragas, M., E. H. Drosinos, E. Taskalidou, and J. Metaxopoulos. 2004. Influence of nutrients on growth and bacteriocin production by Leuconostoc mesenterioides L124 and Lactobacillus curvatus L442. Antonie van Leeuwenhoek 85: 191-198 https://doi.org/10.1023/B:ANTO.0000020291.01957.a2
  20. Mazur, H., A. Konop, and R. Synak. 2001. Indol-3-acetic acid in the culture medium of two axenic green microalgae. J. Appl. Phycol. 13: 35-42 https://doi.org/10.1023/A:1008199409953
  21. Mercenier, A., S. Pavan, and B. Pot. 2003. Probiotics as biotherapeutic agents: Present knowledge and future prospects. Curr. Pharm. Res. 9: 175-191 https://doi.org/10.2174/1381612033392224
  22. Moon, G.-S., C.-H. Kang, Y.-R. Pyun, and W. J. Kim. 2004. Isolation, identification and characterization of a bacteriocinproducing Enterococcus sp. from kimchi and its application to kimchi fermentation. J. Microbiol. Biotechnol. 14: 924-931
  23. Nonomura, T., Y. Matsuda, M. Bingo, M. Onish, K. Mastsuda, S. Harada, and H. Toyoda. 2001. Algicidal effect of 3-(3-indolyl)butanoic acid, a control agent of the bacterial wilt pathogen, Ralstonia solanacearum. Crop Protect. 20: 936-939
  24. Oh, S., M. Kim, J. J. Churey, and R. W. Worobo. 2003. Purification and characterization of a bacteriocin produced by Leuconostoc sp. W65. J. Microbiol. Biotechnol. 13: 680-686
  25. Orosa, M., D. Franqueira, A. Cid, and J. Abalde. 2005. Analysis and enhancement of astaxanthin accumulation in Haematococcus pluvialis. Bioresour. Technol. 96: 373-378 https://doi.org/10.1016/j.biortech.2004.04.006
  26. Parente, E. and C. Hill. 1992. A comparison of factors affecting the production of two bacteriocins from lactic acid bacteria. J. Appl. Bacteriol. 73: 290-298 https://doi.org/10.1111/j.1365-2672.1992.tb04980.x
  27. Park, E.-K., M.-W. Seo, and C.-G. Lee. 2001. Effects of medium compositions for the growth and the astaxanthin production of Haematococcus pluvialis. Kor. J. Microbiol. Biotechnol. 29: 227-233
  28. Reid, G., J. Howard, and B. S. Gan. 2001. Can bacterial interference prevent infection? Trends Microbiol. 9: 424-428 https://doi.org/10.1016/S0966-842X(01)02132-1
  29. Terao, J. 1989. Antioxidant activity of ${\beta}$-carotene-related carotenoids in solution. Lipids 24: 659-661 https://doi.org/10.1007/BF02535085
  30. Yamaguchi, K. 1997. Recent advances in microalgal bioscience in Japan, with special reference to utilization of biomass and metabolites: A review. J. Appl. Phycol. 9: 327-330 https://doi.org/10.1023/A:1007910110045
  31. Yuan, J. P. and F. Chen. 1998. Chromatographic separation and purification of trans-astaxanthin from the extracts of Haematococcus pluvialis. J. Agric. Food Chem. 46: 3371-3375 https://doi.org/10.1021/jf980039b
  32. Caire, G., M. Storni de Cano, M. C. Zaccaro de Mule, R. M. Palma, and K. Colombo. 1997. Exopolysaccharide of Nostoc muscorum (Cyanobateria) in the aggregation of soil particles. J. Appl. Phycol. 9: 249-253 https://doi.org/10.1023/A:1007994425799