Biomedical Science Letters (대한의생명과학회지)
- Volume 12 Issue 2
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- Pages.81-89
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- 2006
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
Molecular Characterization of Ischemia-Responsive Protein 94 (irp94) Response to Unfolded Protein Responses in the Neuron
- Kim Seung-Whan (Department of Emergency Medicine Chungnam National University Hospital) ;
- Kwon Ki-Sang (Department of Anatomy College of Medicine Chungnam National University) ;
- Shin Kee-Sun (Korea Research Institute of Bioscience and Biotechnology) ;
- Kim Seung-Ho (Department of Emergency Medicine, Yonsei University of Medicine) ;
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Kwon O-Yu
(Department of Anatomy College of Medicine Chungnam National University)
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권오유
(충남대학교 의과대학 해부학교실)
- Published : 2006.06.01
Abstract
The ischemia-responsive 94 gene (irp94) encoding a 94 kDa endoplasmic reticulum resident protein was investigated its molecular properties associated with unfoled protein responses. First, the expression of irp94 mRNA was tested after the reperfusion of the transient forebrain ischemia induction at the central nervous system in three Mongolian gerbils. Second, irp94 expression in PC12 cells, which are derived from transplantable rat pheochromocytoma cultured in the DMEM media, was tested at transcriptional and translational levels. The half life of irp94 mRNA was also determined In PC12 cells. Last, the changes of irp94 mRNA expression were investigated by the addition of various ER stress inducible chemicals (A23187, BFA, tunicamycin, DTT and