생명과학회지 (Journal of Life Science)

  • 제16권3호
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  • Pages.447-453
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  • 2006
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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애기장대 histidine kinase 3 (AHK3)의 특성과 결손돌연변이체인 ahk3의 프로테옴 분석

Characterization of Arabidopsis Histidine Kinase 3 and Proteomic Analysis of Its Mutant

  • 양영실 (경상대학교 대학원 응용생명과학부) ;
  • 차준영 (경상대학교 대학원 응용생명과학부) ;
  • 네티 엘마와티 (경상대학교 대학원 응용생명과학부) ;
  • 정민희 (경상대학교 대학원 응용생명과학부) ;
  • 이곤호 (경상대학교 대학원 응용생명과학부) ;
  • 손대영 (경상대학교 대학원 응용생명과학부)
  • Liang Ying-Shi (Division of Applied Life Science, Gyeongsang National University) ;
  • Cha Joon-Yung (Division of Applied Life Science, Gyeongsang National University) ;
  • Ermawati Netty (Division of Applied Life Science, Gyeongsang National University) ;
  • Jung Min-Hee (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee Kon-Ho (Division of Applied Life Science, Gyeongsang National University) ;
  • Son Dae-Young (Division of Applied Life Science, Gyeongsang National University)
  • 발행 : 2006.06.01
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초록

Histidine kinase는 식물의 신호전달기작에서 매우 중요한 역할을 한다. 본 연구에서는 애기장대 histidine kinase 3 (AHK3)의 식물체내에서의 기능을 조사하였으며 이 유전자의 결손 돌연변이체인 ahk3에 trans-zeatin (t-zeatin)을 처리하여 유전자와 단백질의 발현양상을 분석하였다. ahk3는 야생형 식물체에 비하여 캘러스 형성, 유모의 성장, 잎의 노화과정에서 t-zeatin에 대한 감수성이 줄어들었다. 프로테옴 분석 결과 eukaryotic translation initiation factor 5A-2, auxin binding glutathione S-transferase, NDPK1 등은 야생형의 애기장대에서는 t-zeatin에 의하여 발현이 증가하는 반면 ahk3에서는 증가하지 않는 것으로 나타났다. 또한 cytokinin처리에 의하여 발현이 증가하는 것으로 보고된 A-type response regulator들 중에서 ARR4와 ARR16의 발현양이 ahk3에서는 현저하게 감소하는 것으로 나타났다. 이러한 결과들은 AHK3가 cytonin신호전달기작에서 매우 중요한 역할을 하며, 프로테옴 분석에 의하여 동정된 단백질들과 ARR4, ARR16은 AHK3에 의해 매개되는 cytokinin 신호전달과정에서 중요한 역할을 할 것으로 생각된다.

Histidine kinase plays important roles in signal transduction in plant. We characterized the function of Arabidopsis histidine kinase 3 (AHK3) and analyzed the expression patterns of genes and proteins in its mutant ahk3 by trans-zeatin (t-zeatin). The ahk3 exhibited decreased sensitivity to t-zeatin during callus formation, seedling growth, and leaf senescence. From proteomic analysis of ahk3, eukaryotic translation initiation factor 5A-2, auxin binding glutathione S-transferase, and NDPK1 were identified not to be induced by t-zeatin, when compared to the wild-type. In addition, the expression levels of ARR4 and ARR16 among A-type response regulators (ARRs) markedly decreased in ahk3 by t-zeatin treatment. These results suggest that AHK3 plays an important role in cytokinin signaling and the proteins identified from proteomic analysis and specific ARRs, ARR4 and ARR16 may be directly or indirectly associated in AHK3-mediated cytokinin signaling.

키워드

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