Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Screening of an Antagonistic Bacterium for Control of Red-pepper Anthracnose, Colletotrichum gloeosporioides

고추탄저병균 Colletotrichum gloeosporioides의 방제를 위한 길항 미생물의 분리 및 항진균 활성

  • Published : 2006.06.01
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Abstract

Bacillus sp. KMU-991 was isolated from Oslo city soils at Norway and shown a strong antifungal activity on red-pepper anthracnose, Colletotrichum gloeosporioides. Bacillus sp. KMU-991 produced a maximum level of antifungal substrate under aerobic incubation at $30^{\circ}C$, 180 rpm for 48 hours in TSB medium(initial pH 7.0) containing 1.0% mannitol and 1.0% ammonium chloride. Precipitate of culture broth by $30{\sim}60%$ ammonium sulfate precipitation exhibited strong antifungal activity against C. gloeosporioides KACC 40804. Butanol extract of cultured broth also shown fungal growth inhibitory activity against Fusarium oxysporum f. sp. radicus-lycopersici KACC 40537, Rhizoctonia solani AG-4 KACC 40142, Botrytis cinerea KACC 40573, Colletotrichum orbiculare KACC 40808, and Phytophthora cambivora KACC 40160 by agar diffusion method.

노르웨이의 Oslo시 지역의 토양시료로부터 분리된 Bacillus sp. KMU-991를 이용하여 고추탄저병균인 Colletotruchum gloeosporioides KACC 40804에 대한 항진균 활성을 위한 배양 조건을 조사하였다. 항진균 물질의 생산은 기본배지로 TSB를 사용하였으며 탄소원으로 1.0% mannitol과 질소원으로 1.0% ammonium chloride를 첨가하였을 매 가장 높은 항진균 활성을 나타내었다. 배양조건으로는 $30^{\circ}C$, 180 rpm, 48시간 배양하였을 때 가장 높은 항진균 활성을 나타내었다. $30{\sim}60%$ ammonium sulfate 침전물을 첨가하였을 때 가장 양호한 항진균 활성을 나타내었으며 butanol을 이용하여 배양액 중에 존재하는 항진균 물질을 회수하여 다양한 작물병원성 곰팡이에 대한 spectrum을 조사한 결과 B. cinerea KACC 40573, C. orbiculare KACC 40808, F. oxysporum f. sp. radicus-lycopersici KACC 40537, P. cambivora KACC 40160, 그리고 R. solani AG-4 KACC 40142등에 대하여도 높은 항진균 활성을 나타내었다.

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References

  1. Bae, M. 1978. Present status and future of antibiotics for agriculture. Kor. J. Microbiol. Biotechnol. 20, 141-148
  2. Banos, S. B., M. H. Lopez, E. B. Molina and C. L. Wilson. 2003. Effects of chitosan and plant extracts on growth of Colletotrichum gloeosporioides, anthracnose levels and quality of papaya fruit. Crop Protection 22, 1087-1092 https://doi.org/10.1016/S0261-2194(03)00117-0
  3. Chang, J. Y., H. H. Lee, I. C. Kim and H. C. Chang. 2001. Characterization of a bacteriocin produced by Bacillus licheniformis cy2. J. Korean Soc. Food Sci. Nutr. 30, 410-414
  4. Chang, S. H. and B. K. Chung. 1985. Studies on the varietal resistance and effects of nutrients for fungal growth of pepper anthracnose disease caused by Colletotrichum dematium f. sp. capsium. Kor. J. Mycol. 13, 227-233
  5. Cho, S. J., B. J. Cha and K. S. Shin. 2004. Effect of culture parameters on the production of growth inhibitory substance of Colletotrichum gloeosporioides from Bacillus subtilis. Kor. J. Mycol. 32, 138-141 https://doi.org/10.4489/KJM.2004.32.2.138
  6. Cho, S. J., S. K. Lee, B. J. Cha, Y. H. Kim and K. S. Shin. 2003. Detection and characterization of the Cloeosporium gloeosporioides growth inhibitory compound irurin A from Bacillus subtilis strain KS03. FEMS Microbiol. Lett. 223, 47-51 https://doi.org/10.1016/S0378-1097(03)00329-X
  7. Chung, B. K. and S. B. Lee. 1986. Effects of conidial number and nutrition on the germination of conidia in Colletotrichum dematium f. sp. capsium causing red pepper anthracnose. Kor. J. Plant Prot. 25, 41-46
  8. Holt, J. G., N. R. Krieg, P. H. A. Sneath, J. T. Staley and S. T. Williams. 1994. Bergey' Manual of Systematic Bacteriology. 9th. Williams&Wilkins. U.S.A
  9. Jeon, Y. H., S. P. Chang, I. Hwang and Y. H. Kim. 2003. Involvement of growth-promoting Rhizobacterium Paenibacillus polymyxa in root rot of stored korean ginseng. J. Microbiol. Biotechnol. 13, 881-891
  10. Jung, B. K. and S. H. Chang. 1984. An etiological study on the anthracnose fungus of pepper caused by Colletotrichum dematium in Korea. Kor. J. Mycol. 12, 153-157
  11. Jung, H. K and S. D. Kim. 2003. Purification and chracterization of an antifungal antibiotic from Bacillus megaterim KL 39, a biocontrol agent of red-peper phytophthora blight disease. Kor. J. Mircobiol. Biotechnol. 31, 235-241
  12. Kim, J. Y., E. A. Bae, M. J. Han and D. H. Kim. 1999. Inhibitory activity of Bacillus licheniformis AJ on the growth of diarrheal pathogens. J. Appl. Pharm. 7, 385-389
  13. Kim, S. H., Y. D. Lee, W. S. Ha and H. M. Ro. 1998. A study on the transport phenomena of hydrophobic pesticides influenced by the interfaces of groundwater in unsaturated inorganic porous media. J. of KSEE. 20, 1545-1553
  14. Lee, E. T. and S. D. Kim. 2000. Selection and antifungal activity of antagonistic bacterium Pseudomonas sp. 2112 against red-pepper rotting Phytophthora capsici. Kor. J. Microbiol. Biotechnol. 28, 334-340
  15. Lee, K. M., O. M. Lee, M. S. Cha, E. H. Park, G. T. Park, H. J. Son and S. J. Lee. 2002. Production and characteristics of environment friendly antimicrobial substance by Pseudomonas aeruginosa EL-KM. J. of the Environmental Sciences. 11, 33-40 https://doi.org/10.5322/JES.2002.11.1.033
  16. Lee, K. S. 1997. Evaluation on the effects of pesticide residues to agroecosystem in Korea. Kor. J. Environ. Agric. 16, 80-93
  17. Oh, I. S., M. S. In, I. S. Woo, S. K. Lee and S. H. Yu. 1998. Anthracnose of pepper seedling caused by Colletotruchum coccodes(wallr.) hughes. Kor. J. Mycol. 16, 151-156
  18. Oh, Y. K. and J. H. Kim. 1997. Effects of residual organochlorine pesticides in the coastal environment on the Cheju Island. J. KSWQ 13, 317-324
  19. Oh, Y. J. 1992. Studies on the optimization of media composition and cultural conditions for kasugamycin production by Streptomyces kasugansis. Kor. J. Microbiol. Biotechnol. 20, 583-587
  20. Paik, S. B. and D. W. Kim. 1995. Screening for phyllospheral antagonistic microorganism for control of red-pepper anthracnose (Colletotrichum gloeosporioides). Kor. J. Mycol. 23, 190-195
  21. Park, K. S. and C. H. Kim. 1992. Identification, distribution and etiological characteristics of anthracnose fungi of red pepper in Korea. Kor. J. Plant Pathol. 8, 61-69
  22. Park, R. D., K. J. Jo, Y. Y. Jo, Y. L. Jin, K. Y. Kim, J. H. Shim and Y. W. Kim. 2002. Variation of antifungal activities of chitosans on plant pathogens. J. Microbiol. Biotechnol. 12, 84-88
  23. Park, S. M., H. J. Jung, S. H. Han, S. H. Yeo, Y. W. Kim, H. G. Ahn, H. S. Kim and T. S. Yu. 2005. Antifungal activity of extract from Xanthium strumarium L. against plant pathogenous fungi. J. of Life Science 15, 692-695 https://doi.org/10.5352/JLS.2005.15.5.692
  24. Park, S. M., S. H. Han and T. S. Yu. 2005. Culture conditions and antifungal activity of Bacillus licheniformis KMU-3 against crop pathogenic fungi. Kor. J. Microbiol. Biotechnol. 33, 112-116
  25. Park, W. M., Y. S. Lee, S. H. Kim and Y. H. Ko. 1988. Biochemical investigation of resistance of green pepper fruit to Colletotrichum gloeosporioides Penz. Kor. J. Plant Pathol. 4, 290-296

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