Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

The antibacterial effect of photo-catalytic titanium dioxide on canine skin

광 촉매 이산화 티타늄의 개 피부에 대한 항균효과

  • Chang, Hwa-Seok (College of Veterinary Medicine, Konkuk University) ;
  • Kim, Ji-Eun (College of Veterinary Medicine, Konkuk University) ;
  • Chung, Dai-Jung (College of Veterinary Medicine, Konkuk University) ;
  • Lee, Jung-Sun (College of Veterinary Medicine, Konkuk University) ;
  • Choi, Chi-Bong (College of Veterinary Medicine, Konkuk University) ;
  • Kim, Hwi-Yool (College of Veterinary Medicine, Konkuk University)
  • 장화석 (건국대학교 수의과대학) ;
  • 김지은 (건국대학교 수의과대학) ;
  • 정다정 (건국대학교 수의과대학) ;
  • 이정선 (건국대학교 수의과대학) ;
  • 최치봉 (건국대학교 수의과대학) ;
  • 김휘율 (건국대학교 수의과대학)
  • Accepted : 2006.09.14
  • Published : 2006.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Photo-catalytic products have been widely used at home and hospital to prevent bacteria, virus and fungus. Activities of anti-bacteria, anti-viruses and anti-fungi are based upon direct contact of crystals and particles of titanium dioxide with pathogens, into which titanium is catalyzed by photo. Those antimicrobial activities of the photo-catalytic titanium dioxide have been proved in vitro. However, in vivo tests of those activities have not been carried out on dog skin. Aim of this study was to evaluate the antimicrobial activities of the catalytic titanium dioxide in vivo. Ten beagle dogs were divided into two groups. One group was sprayed with 10ml of titanium dioxide(1 mg/ml) whereas the other was not. The treated dogs were exposed under the sunlight for 120 min. A set of three hairs was taken 15, 30, 60 and 120 min after the exposure and the bacteria contaminated in hairs were amplified in, Muller Hilton broth at $35^{\circ}C{\pm}1$ for 3 h. The supernatant of the bacterial culture was diluted 1 : 10 in phosphafe-buffered saline. One milliliter of the diluents was transferred into triphenyltetrazolium medium(TTC) and incubated at $35^{\circ}C{\pm}1$ for 2 days. The number of bacteria was counted. The number of bacteria colonies was decreased compared to control group. To further investigate the longevity effect of titanium dioxide, the dogs were kept in indoor without sun light for 6 and 12 h, 1, 2, 3, 7, 14 days after exposure of the chemical during each 15, 30, 60 min. The number of bacteria colony in 1ml was counted. The number of bacterial colonies was decreased. Treated group is exposured by sun light during 15 min, the longevity effect of titanium dioxide is continued by 1 week. Treated group is exposured by sun light during 30, 60 min, the longevity effect of titanium dioxide is continued over 2 weeks. These data indicated that the photo-catalytic titanium dioxide may be used for prevent bacteria on dog skin.

Keywords

References

  1. Blake DM. Bibliography of work on the photocatalytic removal of hazardous compounds from water and air. pp. 211-213, National Renewable Energy Laboratory, Boulevard, 1994
  2. Blake DM. Bibliography of work on the photocatalytic removal of hazardous compounds from water and air. Update Number 1. pp. 254-256, National Renewable Energy Laboratory, Boulevard, 1995
  3. Blake DM. Bibliography of work on the photocatalytic removal of hazardous compounds from water and air. Update Number 2. pp. 88-89, National Renewable Energy Laboratory, Boulevard, 1997
  4. Blake DM, Pin C, Manass Z, Edward J, Jie Huang. Application of the photocatalytic chemistry of titanium dioxide to disinfection and the killing of cancer cells. Sep Puri Meth 1999, 28, 1-50 https://doi.org/10.1080/03602549909351643
  5. Crosby HA, Bion JF, Penn CW, Elliott TS. Antibiotic-induced release of endotoxin from bacteria in vitro. J Med Microbiol 1994, 40, 23-30 https://doi.org/10.1099/00222615-40-1-23
  6. DeRenzis FA. Endotoxin-inactivating potency of hydrogen peroxide effect on cell growth. J Dent Res 1981, 60, 933-935 https://doi.org/10.1177/00220345810600051401
  7. Fuijishima A, Honda K. Electrochemical photo catalysis of water at semiconductor electrode. Nature 1972, 238, 37-38 https://doi.org/10.1038/238037a0
  8. Hien N, Thomas MD, Ebtisam WJ. Photocatalytically-mediated disinfection of water using $Tio_2$ as a catalyst and spore-forming Bacillus pumilus as a model. Environ Sci Health 1995, 21, 627-636
  9. Homma Y, Matsuura M, Kumazawa Y. Studies on lipid A the active center of endotoxine structure activity relationship. Nippon Saikingaku Zasshi 1989, 44, 585-608 https://doi.org/10.3412/jsb.44.585
  10. Issekutz AC. Removal of gram-negative endotoxin from solutions by affinity chromatography. J Immunol Methods 1983, 31, 275-281
  11. Kotani S, Takada H, Tsujimoto M, Ogawa T, Takahashi I, Ikeda T, Otsuka K, Shimauchi H, Kasai N, Mashimo J. Synthetic lipid A with endotoxic and related biological activities comparable to those of a natural lipid A from an Escherichia coli re-mutant. Infect Immun 1985, 49, 225-237
  12. Matsunaga T, Tomoda R, Nakajima T, Nakamura N, Komine T. Continuous-sterilization system that uses photosemiconductor powders. Appl Environ Microbiol 1988, 54, 1330-1333
  13. Matsunaga T, Tomoda R, Nakajima T, Wake H. Photoelectrochemicalmical sterilization of microbial cells by semiconductor powders. FEMS. Microbiol Let 1985, 29, 211-214 https://doi.org/10.1111/j.1574-6968.1985.tb00864.x
  14. Satio T, Iwase T, Horie J, Morioka T. Mode of Photo catalytic bactericidal action of powdered semiconductor $TiO_2$ on mutans streptococci. J Photoche Photobio 1992, 21, 369-379
  15. Sunada K, Kikuchi Y, Hashimoto K, Fujishima A. Bactericidal and detoxification effects of $TiO_2$ thin film photo catalysts. Environ Sci Tec Biol 1998, 26, 1-21
  16. Wan-Chun C, Ming-Hwa C, Tse-Min L. Incidence of highly genetically diversified Vibrio parahaemolyticus in seafood imported from asian cunntries. Bot Bull Acad Sin 1999, 40, 207-212
  17. Wei C, Lin W, Zulkarnain W, Nathan E, Zhu K. Bactericidal activity of $TiO_2$ photocatalyst in aqueous media toward a solar assisted water disinfection system. Environ Sci Techno 1994, 28, 934-938 https://doi.org/10.1021/es00054a027