Study on Skin Care Properties of Milk Kefir Whey

  • Chen, Ming-Ju (Department of Animal Science, National Taiwan University) ;
  • Liu, J.R. (Department of Biotechnology, National Formosa University) ;
  • Sheu, J.F. (Department of Animal Science, National Taiwan University) ;
  • Lin, C.W. (Department of Animal Science, National Taiwan University) ;
  • Chuang, C.L. (Department of Applied cosmetics, Chih-Kou Institute of Technology)
  • Received : 2005.09.15
  • Accepted : 2006.01.25
  • Published : 2006.06.01


The purpose of this research was to study the effects of kefir whey (kefir whey, peptides, lactic acid) on skin care properties including skin lightening effect and acne treatment. The final aim was to develop a new cosmetic product and enhance the value of dairy products. The results of skin lightening tests showed that all three kefir whey components (kefir whey, peptides and lactic acid) had inhibitory ability against melanin synthesis. Furthermore, copper chelating analysis demonstrated that both kefir whey and kefir whey peptides could chelate the copper in tyrosinase, which might explain the mechanism of inhibition. The ability for acne treatment indicated that lactic acid level higher than 60 mg/ml could inhibit the growth of Propionibacterium acne, whereas no inhibition was found with other components.



Supported by : National Science Council, ROC (Taiwan)


  1. Chen, M. J., J. R. Liu, C. W. Lin and Y. T. Yeh. 2005. Study of the Microbial and chemical properties of goat milk kefir produced by inoculation with Taiwanese kefir grains. Asian-Aust. J. Anim. Sci. 18(5):711-715
  2. Curto, E. V., C. Kwong, H. Hermersdorfer, H. Gant, C. Santis, V. Virador, V. J. Hearing and T. P. Dooley. 1999. Inhibitors of mammalian melanocyte tyrosinase: In vitro comparisons of alkyl esters of gentisic acid withother putative inhibitors. Biochem. Pharmacol. 57:663-672
  3. Dreno, B., A. Reynaud, H. Richet, D. Moyse and H. Habert. 2001. Erythromycin-resistance of cutaneous bacterial flora in acne. Eur. J. Dermatol. 11:549-553
  4. Eady, E. A., E. E. Jones and J. L. Tipper. 1993. Antibiotic resistant propionibacteria in acne: need for policies to modify antibiotic usage. Br. Med. J. 306:555-556
  5. Higaki, S. 2003. Lipase inhibitors for the treatment of acne. J. Mol. Catal. B 22:377-384
  6. Kubo, I. and I. Kinst-Hori. 1999. Flavonols from Saffron Flower: Tyrosinase inhibitory activity and inhibition mechanism. J. Agri. Food Chem. 47:4121-4125
  7. Lin, C. W., H. L. Chen and J. R. Liu. 1999. Identification and characterization of lactic acid bacteria and yeasts isolated from kefir grains in Taiwan. Aust. J. Dairy Technol. 54:14-18
  8. Lintner, K. and O. Peschard. 2000. Biologically active peptides: from a laboratory bench curiosity to a functional skin care product. Inter. J. Cosmetic Sci. 22:207-218
  9. Liu, J. R., Y. Y. Lin, M. J. Chen, L. Y. Chen and C. W. Lin. 2005. Antioxidative activities of kefir. Asian-Aust. J. Anim. Sci. 18:567-573
  10. Liu, J. R., M. J. Chen and C. W. Lin. 2005. Antimutagenic and Antioxidant properties of milk kefir and soymilk kefir. J. Agr. Food Chem. 53:2467-2474
  11. Maeda, K. and M. Fukuda. 1996. Arbutin: Mechanism of its depigmenting action in human melanocyte culture. J. Pharmacol. Exp. Ther. 276:765-769
  12. Mitusuhashi, S. and N. Murata. 1991. Inhibitory activity of Bifidobacterium on the growth of Gram-negative and Grampositive bacteria. J. Jpn. Soc. Nutr. Food Sci. 44:365-372
  13. Montgomery, D. C. 1991. Experimented with a single factor: the analysis of varience. In Design and Analysis of Experiments, (Ed. D. C. Montgomery), pp. 75-77. John Wiley & Sons, New York
  14. Nishijima, S., H. Akamatsu and M. Akamatsu. 1994. The antibiotic susceptibility of Propionibacterium acnes and Staphylococcus epidermidis isolated from acne. J. Dermatol. 21:166-171
  15. Park, H., J. Perez, R. Laursen and B. Gilchrest. 1998. A tyrosinase mimetic peptide inhibits tyrosinase activity in cultured human melanocytes. J. Dermatol. Sci. 16:133
  16. Sanchez-Ferrer, A., J. N. Rodriuez-Lopez and Garcia-Canovas. 1995. Tyrosinase: a comprehensive review of its mechanism. Biochim. Biophys. Acta. 1247:1-11
  17. SAS/STAT. 1999. SAS/STAT User's guide. SAS Institute Inc., Cary, NC
  18. Shimada, K., K. Fujikawa, K. Yahara and T. Nakamura. 1992. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J. Agric. Food Chem. 40:945-948
  19. Usuki, A., A. Ohashi, H. Sato, Y. Ochiai, M. Ichihashi and Y. Funasaka. 2003. The inhibitory effect of glycolic acid and lactic acid on melanin synthesis in melanoma cells. Exp. Dermatol. 2:43-50
  20. Wang, C. M., C. L. Huang, C. T. S. Hu and H. L. Chan. 1997. The effect of glycolic acid on the treatment of acne in Asian skin. Dermatol. Surg. 23:23-29

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