Asian-Australasian Journal of Animal Sciences

  • 제19권5호
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  • Pages.617-621
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  • 2006
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Analysis of Genetic Diversity of the Thai Swamp Buffalo (Bubalus bubalis) Using Cattle Microsatellite DNA Markers

  • Triwitayakorn, K. (Institute of Molecular Biology and Genetics, Mahidol University) ;
  • Moolmuang, B. (Institute of Molecular Biology and Genetics, Mahidol University) ;
  • Sraphet, S. (Institute of Molecular Biology and Genetics, Mahidol University) ;
  • Panyim, S. (Institute of Molecular Biology and Genetics, Mahidol University) ;
  • Na-Chiangmai, A. (Department of Livestock Development) ;
  • Smith, Duncan R. (Institute of Molecular Biology and Genetics, Mahidol University)
  • 투고 : 2005.08.04
  • 심사 : 2005.11.16
  • 발행 : 2006.05.01
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초록

Recently the numbers of the Thai swamp buffalo (Bubalus bubalis), a native species of Thailand, have been rapidly declining, leading to a requirement for conservation programs for this breed. Such studies of the genetic diversity of this species are essential for conservation decisions and to assist the rational implementation of breeding programs. In this study, the genetic diversity of 80 Thai swamp buffalo, randomly selected from seven different research stations of the Thai Department of Livestock Development, were studied using ten cattle microsatellite markers. Polymorphic PCR products were observed at all microsatellite loci, with percentages of polymorphic loci ranging from 80.00 to 100.00%. The population from Payao showed the lowest level of polymorphism. The mean number of alleles per locus was 4.7 with the highest number of alleles being eight (ETH152) and the lowest being three (HAUT27 and ILSTS030). The average unbiased heterozygosity for all seven populations was 0.61 and varied between 0.5314 (Samui) and 0.6798 (Surin). The genetic distance according to NEI's (1972) ranged from 0.0722 to 0.4427. The populations from Surin and Burirum are the closest populations, while populations from Samui and Payao are the most divergent. The information generated by this study will greatly aid in the establishment of effective breeding and conservation programs for the Thai swamp buffalo.

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참고문헌

  1. Barendse, W., S. M. Armitage, L. M. Kosssrek, A. Shalom, B. W. Kirkpatrick, A. M. Ryan, D. Clayton, L. Li, H. L. Neibergs, N. Zhang, W. M. Grosse, J. Weiss, P. Creighton, F. McCarthy, M. Ron, A. J. Teale, R. Fries, R. A. McGraw, S. S. Moore, M. Georges, J. E. Soller, D. J. Womack and S. Hetzel. 1994. A genetic linkage map of the bovine genome. Nat. Genet. 6:227- 235 https://doi.org/10.1038/ng0394-227
  2. Barendse, W., D. Vaiman, S. J. Kemp, Y. Sugimoto, S. M. Armitage, J. L. Williams, H. S. Sun, A. Eggen, M. Agaba, S. A. Aleyasin, M. Band, M. D. Bishop, J. Buitkamp, K. Byrne, F. Collins, L. Cooper, W. Coppettiers, B. Denys, R. D. Drinkwater, K. Easterday, C. Elduque, S. Ennis, G. Erhardt, L. Ferretti, N. Flavin, Q. Gao, M. Georges, R. Gurung, B. Harlizius, G. Hawkins, J. Hetzel, T. Hirano, D. Hulme, C. Jorgensen, M. Kessler, B. W. Kirkpatrick, B. Konfortov, S. Kostia, C. Kuhn, J. A. Lenstra, H. Leveziel, H. A. Lewin, B. Leyhe, L. Lil, I. M. Burriel, R. A. McGraw, J. R. Miller, D. E. Moody, S. S. Moore, S. Nakane, I. J. Nijman, I. Olsaker, D. Pomp, A. Rando, M. Ron, A. Shalom, A. J. Teale, U. Thieven, B. G. D. Urquhart, D. I. Vage, A. VandeWeghe, S. Varvio, R. Velmala, J. Vikki, R. Weikard, C. Woodside, J. E. Womack, M. Zanotti and P. Zaragoza. 1997. A medium-density genetic linkage map of the bovine genome. Mamm Genome. 8:21-28 https://doi.org/10.1007/s003359900340
  3. Barker, J. S., S. S. Moore, D. J. Hetzel, D. Evans, S. G. Tan and K. Byren. 1997a. Genetic diversity of Asian water buffalo (Bubalus bubalis): microsatellite variation and a comparison with protein-coding loci. Anim. Genet. 28:103-115 https://doi.org/10.1111/j.1365-2052.1997.00085.x
  4. Barker, J. S., S. G. Tan, O. S. Selvaraj and T. K. Mukherjee. 1997b. Genetic variation within and relationships among populations of Asian water buffalo (Bubalus bubalis). Anim. Genet. 28:1- 13 https://doi.org/10.1111/j.1365-2052.1997.00036.x
  5. Bishop, M. D., S. M. Kappes, J. W. Keele, R. W. Stone, S. L. F. Sunden, G. A. Hawkins, S. S. Toldo, R. Fries, M. D. Grosz, J. Yoo and C. W. Beattie. 1994. A genetic linkage map for cattle. Genet. 136:619-639
  6. Chen, G. H., X. S. Wu, D. Q. Wang, J. Qin, S. L. Wu, Q. L. Zhou, F. Xie, R. Cheng, Q. Xu, B. Liu, X. Y. Zhang and O. Olowofeso. 2004. Cluster analysis of 12 Chinese Native chicken populations using microsatellite markers. Asian-Aust. J. Anim. Sci. 17:1047-1052 https://doi.org/10.5713/ajas.2004.1047
  7. Food and Agriculture Organization of the United Nations. 2004. FAOSTAT data: http://faostat.fao.org
  8. Indramangala, J. 2001. Buffalo development in Thailand; Development strategies for genetic evaluation for beef production in developing countries. In: Proceedings of an International Workshop, Khon Kaen Province, Thailand. 108:117-123
  9. Kappes, S. M., J. W. Keele, R. T. Stone, R. A. McGraw, T. S. Sonstegard, T. P. Smith, N. L. Lopez-Corrales and C. W. Beattie. 1997. A second generation linkage map of the bovine genome. Genome. Res. 7:235-249 https://doi.org/10.1101/gr.7.3.235
  10. Kim, K. S., J. S. Yeo and C. B. Choi. 2001. Genetic diversity of north-east Asian cattle based on microsatellite data. Anim. Genet. 33:201-204 https://doi.org/10.1046/j.1365-2052.2002.00848.x
  11. Macgregor, R. 1941. The domestic water buffalo. Cited in Mason I.L. In The Husbandry and Health of the Domestic Buffalo, FAO, Roma, pp. 57-81
  12. MacHugh, D. E., M. D. Shriver, R. T. Loftus, P. Cunningham and D. G. Bradley. 1997. Microsatellite DNA variation and the evolution, domestication and phylogeography of Taurine and Zebu cattle (Bos taurus and Bos indicus). Genet. 146:1071- 1086
  13. Martin-Burriel, I., E. Garcia-Muro and P. Zaragoza. 1999. Genetic diversity analysis of six Spanish native cattle breeds using microsatellites. Anim. Genet. 30(3):177-182 https://doi.org/10.1046/j.1365-2052.1999.00437.x
  14. Miller, M. P. 1997. Tools for population genetic analysis. Version 1.3. Dept of Biological Sciences, Northern Arizona Univ., Flagstaff, AZ
  15. Moore, S. S., D. Evan, K. Byrne, J. S. Barker, S. G. Tan, D. Vankan and D. J. Hetzel. 1995. A set of polymorphic DNA microsatellites useful in swamp and river buffalo (Bubalus bubalis). Anim. Genet. 26:355-359 https://doi.org/10.1111/j.1365-2052.1995.tb02674.x
  16. Na-Chiangmai, A. 2001. Current situation and development trends of beef production in Thailand. Development strategies for genetic evaluation for beef production in developing countries. In Proceedings of an International Workshop, Khon Kaen Province, Thailand. 108:93-97
  17. Navani, N., P. K. Jain, S. Gupta, B. Sisodia and S. Kumar. 2001. A set of cattle microsatellite DNA markers for genome analysis of riverine buffalo (Bubalus bubalis). Anim. Genet. 33:149- 154 https://doi.org/10.1046/j.1365-2052.2002.00823.x
  18. Nei, M. 1972. Genetic distance between populations. Am. Nat. 106:283-92 https://doi.org/10.1086/282771
  19. Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genet. 89:583-92
  20. Osman, S. A.-M., M. Sekino, M. Nishibori, Y. Yamamoto and M. Tsudzuki. 2005. Genetic variability and relationships of native Japanese chickens assessed by microsatellite DNA profiling - focusing on the breeds established in Kochi Prefecture, Japan-. Asian-Aust. J. Anim. Sci. 18:755-761 https://doi.org/10.5713/ajas.2005.755
  21. Selvi, P. K., J. M. Panandam, K. Yusoff and S. G. Tan. 2004. Molecular characterisation of the Mafriwal Dairy Cattle of Malaysia using microsatellite markers. Asian-Aust. J. Anim. Sci. 17:1366-1368 https://doi.org/10.5713/ajas.2004.1366
  22. Steffen, P., A. Eggen, A. B. Dietz, J. E. Womack, G. Stranzinger and R. Fries. 1993. Isolation and mapping of polymorphic microsatellites in cattle. Anim. Genet. 24:121-124 https://doi.org/10.1111/j.1365-2052.1993.tb00252.x
  23. Sun, W., H. Chang, Z. J. Ren1, Z. P. Yang, R. Q. Geng, S. X. Lu, L. Du and K. Tsunoda. 2004. Genetic differentiation between sheep and goats based on microsatellite DNA. Asian-Aust. J. Anim. Sci. 17:583-587 https://doi.org/10.5713/ajas.2004.583
  24. The Department of Livestock Development, Thailand: http:// www.dld.go.th/ict/yearly/yearly47/yearly47.html
  25. Van Hooft, W. F., A. F. Groen and H. H. Prins. 2000. Microsatellite analysis of genetic diversity in African buffalo (Syncerus caffer) population throughout Africa. Mol Ecol. 9:2017-2025 https://doi.org/10.1046/j.1365-294X.2000.01101.x
  26. Yang, Z. P., H. Chang, W. Sun, R. Q. Gen, Y. J. Mao and K. Tsunoda. 2004. A comparison of two kinds of markers applied in analysis of genetic diversity in sheep and goat populations. Asian-Aust. J. Anim. Sci. 17:892-896 https://doi.org/10.5713/ajas.2004.892

피인용 문헌

  1. Use of Cattle Microsatellite Markers to Assess Genetic Diversity of Thai Swamp Buffalo (Bubalus bubalis) vol.21, pp.2, 2006, https://doi.org/10.5713/ajas.2008.70327
  2. Whole-Genome Resequencing Reveals Adaptation Prior to the Divergence of Buffalo Subspecies vol.13, pp.1, 2006, https://doi.org/10.1093/gbe/evaa231
  3. Comparative Growth and Economic Performances between Indigenous Swamp and Murrah Crossbred Buffaloes in Malaysia vol.11, pp.4, 2006, https://doi.org/10.3390/ani11040957
  4. The Impact of Feed Supplementations on Asian Buffaloes: A Review vol.11, pp.7, 2006, https://doi.org/10.3390/ani11072033