Comparison of Cell Wall Ultrastructures of Aspergillus nidulans in Presence and Absence of a MnpAp Mannoprotein

  • Jeong, Hyo-Yong (Division of Biological Sciences, Chonbuk National University) ;
  • Whang, Sung-Soo (Division of Science Education, Chonbuk National University) ;
  • Chae, Keon-Sang (Division of Biological Sciences, Chonbuk National University)
  • Published : 2006.09.30

Abstract

The ultrastructure of Aspergillus nidulans cell wall in relation to a mannoprotein was studied by scanning and transmission electron microscopy. An mnpAp null-mutant, DMPV1, was used as a negative control of a wild type VER7. To analyze whether the mannoprotein in the cell wall during the development of an mnpAp null-mutant is present or not, immunogold microscopy was also adopted. The surface sculpturing of various cell types - hyphae, conidium, Hulle cell, and ascospore - were not very different between the wild type and the mnpAp-null mutant (DMPV1) as examined by scanning electron microscopy. These results were comparable to those examined by transmission electron microscopy, in that the hyphal cell wall was not indentical between two strains, probably caused by the MnpA protein (MnpAp). MnpAp was absent in both the hyphal cell wall of the DMPV1 strain and the conidial cell wall of a wide type, but clearly recognized in the hyphal cell wall of a wild type.

Keywords

References

  1. Adams TH, Wieser JK, and Yu J-H (1998) Asexual sporulation in Aspergillus nidulans. Microbiol Mol Biol Rev 62: 35-54
  2. Aronson JM (1981) Cell wall chemistry, ultrastructure and metabolism. In: Cole GT (ed), Biology of Conidial fungi, Vol. 2, Academic Press, London, pp 459-507
  3. Bidard F, Blondin B, Dequin S, Vezinhet F, and Barre P (1994) Cloning and analysis of a FLO5 flocculation gene from S. cerevisiae. Cur Gene 25: 196-201 https://doi.org/10.1007/BF00357162
  4. Bogia PT (1992) The role of the orlA, tsE and bimG genes of Aspergillus nidulans in chitin synthesis. J Bacteriol 174: 384-389 https://doi.org/10.1128/jb.174.2.384-389.1992
  5. Borgia PT, and Dordge CL (1992) Characterization of Apergillus nidulans mutants deficient in cell-wall chitin or glucan. J Bacteriol 174: 377-383 https://doi.org/10.1128/jb.174.2.377-383.1992
  6. Bowen AR, Chen-Wu JL, Momany M, Young R, Szaniszlo PJ, and Robbins PW (1992) Classification of fungal chitin synthesis. Proc Nat Acad Sci USA 89: 519-523 https://doi.org/10.1073/pnas.89.2.519
  7. Bull AT (1970) Chemical composition of wild-type and mutant Aspergillus nidulans cell walls. The nature of polysaccharide and melanin constituents. J Gene Microbiol 63: 75-94 https://doi.org/10.1099/00221287-63-1-75
  8. Cao L, Chen DL, Lee C, Chan CM, Chan KM, Vanittanakom N, Tsang DNC, and Yuen KY (1998) Detection of specific antibodies against an antigenic mannoprotein for the diagnosis of penicilliosis marneffei. J Clinical Microbiol 36: 3028-3031
  9. Caro LHP, Tettelin H, Vossen JH, Ram AF,van den Ende H, and Klis FM (1997) In silicio indentification of glycosylphosphatidylinositol-anchored plasma-membrane and cell wall proteins of Saccharomyces cerevisiae. Yeast 13: 1477-1489 https://doi.org/10.1002/(SICI)1097-0061(199712)13:15<1477::AID-YEA184>3.0.CO;2-L
  10. Claverie-Martin F, Diaz-Torres MR, and Geoghegan MJ (1988) Chemical composition and ultrastructure of wild-type and white mutant Aspergillus nidulans conidial walls. Cur Microbiol 16: 281-287 https://doi.org/10.1007/BF01568692
  11. Doonan JH (1994) Control of cell growth. In Aspergillus: 50 Years on. Elsevier, Amsterdam, pp 455-478
  12. Fontaine T, Simenel C, Dubreucq G, Adam O, Delepierre M, Lemoine J, Vorgias CE, Diaquin M, and Latge JP (2000) Molecular organization of the alkali-insoluble fraction of Aspergillus fumigatus cell wall. J Biol Chem 275: 27594-27607 https://doi.org/10.1074/jbc.M909975199
  13. Fujiwara M, Horiuchi H, Ohta A, and Takagi M (1997) A novel fungal gene encoding chitin synthase with a myosin motorlike domain. Biochem Biophy Res Comm 236: 75-78 https://doi.org/10.1006/bbrc.1997.6907
  14. Han K-H, Han K-Y, Yu J-H, Chae K-S, Jahng K-Y, and Han D-M (2001) The nsdD gene encodes a putative GATA type transcription factor necessary for sexual development of Aspergillus nidulans. Mol Microbiol 41: 299-309 https://doi.org/10.1046/j.1365-2958.2001.02472.x
  15. Hearn VM (1997) Structure and function of fungal cell wall. In: Jacobs PH and NaIl L (eds), Fungal Diseases, Marcel Dekker Press, New York, pp 27-60
  16. Hearn VM, Cole GT, and Susuki SS (1993) Fungal antigens. In: Van Regenmortel MHV (ed), Structure of Antigens, CRC Press Inc, New York, pp 211-260
  17. Jeong H-Y, Chae K-S, and Whang SS (2004) Presence of a mannoprotein, MnpAp, in the hyphal cell wall of Aspergillus nidulans. Mycologia 96: 52-56 https://doi.org/10.2307/3761987
  18. Jeong H-Y, Han D-M, Jahng K-Y, and Chae KS (2003) Expression of the mnpA Gene that Encodes the Mannoprotein of Aspergillus nidulans is Dependent on fadA and flbA as Well as veA. Fungal Gene Biol 38: 228-236 https://doi.org/10.1016/S1087-1845(02)00527-3
  19. Jeong H-Y, Han D-M, Jahng K-Y, and Chae K-S (2000) The rp116a gene for ribosomal protein Ll6A identified from expressed sequence tags is differentially expressed during sexual development of Aspergillus nidulans. Fungal Gene Biol 31: 69-78 https://doi.org/10.1006/fgbi.2000.1233
  20. Kaminskyj S, and Hamer J (1996) A medium for rapid cleaning of Aspergillus cleistothecia. Fungal Gene Newsletter 43: 71
  21. Kapteyn JC, Hoyer LL, Hecht JE, Muller WH, Andel A, Verkleij AJ, Makarow M, van den Ende H, and Klis FM (2000) The cell wall architecture of Candida albicans wild-type cells and cell wall defective mutants. Mol Microbiol 35: 601-611 https://doi.org/10.1046/j.1365-2958.2000.01729.x
  22. Klis FM (1994) Review: cell wall assembly in yeast. Yeast 10: 851-869 https://doi.org/10.1002/yea.320100702
  23. Kowalski LRZ, Kondo K, and Inouye M (1995) Cold-shock incubation fo a family of TIP1-related proteins associated with the membrane in Saccharomyces cerevisiae. Mol Microbiol 15: 341-353 https://doi.org/10.1111/j.1365-2958.1995.tb02248.x
  24. Lee D-W, Kim S, Kim S-J, Han D-M, Jahang K-Y, and Chae K-S (2001) The IsdA gene is necessary for sexual development inhibition by a salt in Aspergillus nidulans. Cur Genet 39: 237-243 https://doi.org/10.1007/s002940100206
  25. Lipke PN, Wojciechowicz D, and Kurjan J (1989) Agalpha1 is the structural gene for the Saccharomyces cerevisiae alphaagglutinin, a cell surface glycoprotein involved in cell-cell interactions during mating. Mol Cell Biol 9 : 3155-3165 https://doi.org/10.1128/MCB.9.8.3155
  26. Lo W-S, and Dranginis AM (1996) FLO11, a yeast gene related to the STA genes, encodes a novel surfacefloculin. J Bacteriol 178: 7144-7151 https://doi.org/10.1128/jb.178.24.7144-7151.1996
  27. Lpez-Ribot JL, Sepuveda P, Cervera AM, Roig P, Gozalbo D, and Martinez JP (1997) Cloning of a cDNA fragment encoding part of the protein moiety of the 58-kDa fibriogen-binding mannoprotein of Candida albicans. FEMS Microbiol Letters 157: 273-278 https://doi.org/10.1016/S0378-1097(97)00486-2
  28. Marguet D, Guo XG, and Lauquin GJM (1988) Yeast gene SRP1 (serine-rich protein). Intragenic repeat structure and identification of a family of SRP1-related DNA sequences. J Mol Biol 202: 341-353 https://doi.org/10.1016/0022-2836(88)90278-1
  29. Martinelli SD, and Kinghorn JR (1994) Aspergillus: 50 Years on. Elsevier Science BV, Amsterdam
  30. Moukadiri I, Armero J, Abad A, Sentandreu R, and Zueco J (1997) Identification of a mannoprotein present in the inner layer of the cell wall of Saccharomyces cerevisiae. J Bacteriol 179: 2154-2162 https://doi.org/10.1128/jb.179.7.2154-2162.1997
  31. Nam JS, Lee DH, Lee KH, Park HM, and Bae KS (1998) Cloning and phylogenetic analysis of chitin synthase genes from the insect pathogenic fungus, Matarhizium anisopliae var. anisopliae. FEMS Microbiol Letters 159: 77-84 https://doi.org/10.1111/j.1574-6968.1998.tb12844.x
  32. Pontecorve G, Roper JA, Hemmons LM, MacDonald KD, and Bufton AWJ (1953) The genetics of Aspergillus nidulans. Adv Gene 5: 141-238 https://doi.org/10.1016/S0065-2660(08)60408-3
  33. Roy A, Lu CF, Marykwas DL, Lipke PN, and Kurjan J (1991) The AGA1 product is involved in cell surface attachment of the Saccharomyces cerevisiae cell adhesion glycoprotein aagglutinin. Mol Cell Biol 11 : 4196-4206 https://doi.org/10.1128/MCB.11.8.4196
  34. Smith DJ, and Payton MA (1994) Hyphal tip expression in Aspergillus nidulans requires in manA gene, which encodes phosphomannose isomerase. Mol Cell Biol 14: 6030-6038 https://doi.org/10.1128/MCB.14.9.6030
  35. Specht CA, Liu Y, Robbins PW, Bulawa CE, lartchouk N, Winter KR, Riggle PJ, Rhodes JC, Dodge CL, Culp DW, and Borgia PT (1996) The chsD and chsE genes of Aspergillus nidulans and their roles in chitin synthesis. Fungal Gene Biol Newsletters 20: 153-167 https://doi.org/10.1006/fgbi.1996.0030
  36. Teunissen AWRH, Holub E, Vanderhucht J, Vandenberg JA, and Steensma HY (1993) Sequence of the open reading frame of the FLO1 gene from Saccharomyces cerevisiae. Yeast 9: 423-427 https://doi.org/10.1002/yea.320090413
  37. Van Der Vaart JM, Caro LHP, Chapman JW, Klis FM, and Verrips CT (1995) Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae. J Bacteriol 177: 3104-3110 https://doi.org/10.1128/jb.177.11.3104-3110.1995
  38. Van Der Vaart JM, Van Schagen FS, Mooren ATA, Chapman JW, Klis FM, and Verrips CT (1996) The retention mechanism of cell wall propeins in Saccharomyces cerevisiae. Wall-bound Cwp2p is ${\beta}$1,6-glucosylated. Biochim Biophy Acta 1291: 206-214 https://doi.org/10.1016/S0304-4165(96)00067-0
  39. Van Der Vaart JM, Biesebeke R, Chapman JW, Toschka HY, Klis FM, and Verrips CT (1997) Comparison of cell wall proteins of Saccharomyces cerevisiae as anchors for cell surface expression of heterologous proteins. Applied Environ Biotech 63: 615-620
  40. Zonneveld BJM (1971) Biochemical analysis of the cell wall of Aspergillus nidulans. Biochim Biophy Acta 249: 506-514 https://doi.org/10.1016/0005-2736(71)90126-X