Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Time-course Responses of Hepatic Xenobiotic Metabolizing Enzymes and Stress in Olive Flounder (Paralichthys olivaceus) Exposed to Formalin

Formalin에 약욕시킨 넙치(Paralichthys olivaceus)의 스트레스 반응과 간장 약물대사효소의 경시적 반응

  • Lee Ji-Seon (Division of Marine Bioscience and Technology, Kangnung National University/East coastal Marine Bioresources Research Center) ;
  • Kim Pyong-Kih (Department of Marine Bio-resources, Gangwon Provincial College) ;
  • Lee Kyoung-Seon (Division of Ocean System Engineering, Mokpo National Maritime University) ;
  • Jeon Joong-Kyun (Division of Marine Bioscience and Technology, Kangnung National University/East coastal Marine Bioresources Research Center)
  • 이지선 (강릉대학교 해양생명공학부.동해안해양생물자원연구센터) ;
  • 김병기 (강원도립대학 해양생물자원개발과) ;
  • 이경선 (목포해양대학교 해양환경공학) ;
  • 전중균 (강릉대학교 해양생명공학부.동해안해양생물자원연구센터)
  • Published : 2006.05.01
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Abstract

The effects of formalin on mixed function oxygenase (MFO) system and stress-response were investigated in olive flounder (Paralichthys olivaceus). Olive flounder was exposed to formalin at the concentration of 300 ppm for 1, 2, 4 and 16 h. Levels of stress-response enzymes together with total protein, glucose and osmolality were quantitatively determined in blood, and the activities of phase I (cytochrome P450, ethoxyresorufin deethylase) and phase II (glutathione S-transferase) hepatic enzymes were also determined. Since the formalin-exposure for 16 h resulted no significant changes in aspartate aminotransferase and alanine aminotransferase, specific enzymes for liver damage, it was thought that it did not cause hepatic tissue damage at the concentration of 300 ppm. However, hepatic MFO system was induced at 1 to 4 h, and stress response was induced after 16 h of exposure. Moreover, it is considered that the depression of MFO activity after 16 h of exposure may not be adaptation to formalin, but toxic response. These results suggest that low concentration of formalin does not cause hepatic tissue damage of fish, but could induce MFO and stress response.

Keywords

References

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