Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Effect of Sarcotride A on Membrane Potential in C6 Glioma Cells

  • Lee Yun-Kyung (Laboratories of Pharmacology, College of Pharmacy and Research Institute for Drug Development, Pusan National University) ;
  • Liu Yong-Hong (Marine Natural Product, College of Pharmacy and Research Institute for Drug Development, Pusan National University) ;
  • Jung Jee-H. (Marine Natural Product, College of Pharmacy and Research Institute for Drug Development, Pusan National University) ;
  • Im Dong-Soon (Laboratories of Pharmacology, College of Pharmacy and Research Institute for Drug Development, Pusan National University)
  • Published : 2006.06.01
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Abstract

We tested effect of sarcotride A, a bioactive cyclitol derivative from a marine sponge, on membrane potential in C6 glioma cells. Membrane potential was estimated by measuring fluorescence change of DiBAC-loaded glioma cells. Sarcotride A increased membrane potential in a concentration-dependent manner. We tested effects of pertussis toxin, U73122, EIPA, and $Na^+-free$ media on sarcotride A-induced increase of membrane potential to investigate involvement of G proteins, phospholipase C, $Na^+/H^+$ exchanger, and $Na^+$ channels. However, we were not able to observe any significant effect of those pharmacological inhibitors, excluding the involvement of the molecules as candidate targets or signaling molecules of sarcotride A-induced increase of membrane potential. Further investigation is necessary to elucidate action mechanism of sarcotride A.

Keywords

References

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