아연의 체내축적이 대복의 생존, 운동성, 성장 및 기관계 구조에 미치는 영향

Effect of Zinc Bioaccumulation on Survival Rate, Activity, Growth and Organ Structure of the Equilateral Venus, Gomphina veneriformis (Bivalvia: Veneridae)

  • 주선미 (전남대학교 수산해양대학 수산생명의학과) ;
  • 이재우 (전남대학교 수산해양대학 수산생명의학과) ;
  • 진영국 (전남대학교 수산해양대학 수산생명의학과) ;
  • 유준 (국립수산과학원) ;
  • 이정식 (전남대학교 수산해양대학 수산생명의학과)
  • Ju Sun-Mi (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chunnam National University) ;
  • Lee Jae-Woo (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chunnam National University) ;
  • Jin Young-Guk (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chunnam National University) ;
  • Yu Jun (National Fisheries Research and Development Institute) ;
  • Lee Jung-Sick (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chunnam National University)
  • 발행 : 2006.06.01

초록

This study was conducted to find out survival rate, activity, growth and change of the organ structure of bivalves exposed to heavy metal. The results of the study confirmed that zinc (Zn) induces reduction of survival rate and activity, abnormality of organ structure of the equilateral venus, Gomphina veneriformis. Experimental groups were composed of one control condition and three Zn exposure conditions ($0.64mg\;Zn{\iota}^{1},\;1.07mg\;Zn{\iota}^{-1},\;1.79mg\;Zn{\iota}^{-1}$). As the concentration of zinc increased the accumulation of lipofucin increased in the digestive gland. Survival rate was the lowest in the lowest Zn exposure group at $0.64mg;Zn{\iota}^{-1}$. Growth was not significantly different between the control and exposure group. Activity. with the exception of the lowest Zn exposure group at $0.64mg\;Zn{\iota}^{-1}$, was similar between the control and exposure group. Histological analysis of organ system illustrated expansion of hemolymph sinus, loss of striated border of inner epidermis, increase in the number of mucous cell in the mantle. Also, histological degenerations as epithelial necrosis and hyperplasia of mucous cells are recognized in the gill and foot.

키워드

참고문헌

  1. Abel PD. Effects of some pollutants on the filtration rate of Mytilus edulis, Mar Poll Bull 1976; 7: 228-231 https://doi.org/10.1016/0025-326X(76)90267-8
  2. Akberali HB, Wong TM and Trueman ER. Behavioral and siphonal tissue responses of Scrobicularia plana (Bivalvia) to zinc, Mar Environ Res 1981; 5: 251-264 https://doi.org/10.1016/0141-1136(81)90009-X
  3. Bustamante P and Miramand P. Subcellular and body distribution of 17 trace elements in the variegated scallop, Chlamys varia from the French coast of the bay of Biscay, Sci Total Environ 2005; 337: 59-73 https://doi.org/10.1016/j.scitotenv.2004.07.004
  4. Bryan GW, Langston WJ, Hummerstone and Burt GR. A guide to the assessment of heavy-metal contamination in estuaries using biological indicators, Mar Biol Assoc UK 1985; 4: 1-92
  5. Byrne PA and O'Halloran J. The role of bivalve molluscs as tools in estuarine sediment toxicity testing: a review, Hydrobiologia 2001; 465: 209-217 https://doi.org/10.1023/A:1014584607501
  6. Depledge MH and Hopkin SP. Methods to assess effects on brackish, estuarine and near-coastal water organisms, In: Methods to Assess the Effects of Chemicals on Ecosystems Chap. 7, Lindhurst RA, Bourdeau P and Tardiff RG eds. John Wiley London 1995; pp. 125-149
  7. Depledge MH, Amaral-Medes JJ, Daniel B, Halbrook R, Kloepper-Sams P, Moore MM and Peakall DB. The conceptual basis of the biomarker approach, In: Biomarkers, Research and Application in the Assessment of Environmental Health NATO ASI Series, Peakall H and Shugart RL eds. Springer Berlin 1992; 68: 15-29
  8. Eble AF. Anatomy and histology of Mercenaria mercenaria, In: Biology of the Hard Clam. Kraeuter JN and Castagna M eds. Elsevier Science BV New York 2001; pp. 117-220
  9. Gregory MA, George RC, Marshall DJ, Anandraj A and Mcclurg TP. The effects of mercury exposure on the surface morphology of gill filament in Perna perna (Mollusca: Bivalvia), Mar Poll Bull 1999; 39(1): 116-121 https://doi.org/10.1016/S0025-326X(99)00119-8
  10. Hebel DK, Jones MB and Depledge MH. Responses of crustaceans to contaminant exposure: a holistic approach, Estuar Csttl Shelf Sci 1997; 44: 177-184 https://doi.org/10.1006/ecss.1996.0209
  11. Hemelraad J, Holwerda DA and Zandee DI, Cadmium kinetics in freshwater clams, 1. The pattern of cadmium of accumulation in Anodonta cygnea, Arch Environcation Contam Toxicol 1986; 15(1): 1-7 https://doi.org/10.1007/BF01055243
  12. Jenner HA, Hemelraad J, Marquenie JM and Nopert F. Cadmium kinetics in freshwater clams (Unionidae) under field and laboratory conditions, Sci Total Environ 1991; 108: 205-2142 https://doi.org/10.1016/0048-9697(91)90357-K
  13. Kim SY and Lee TY. The effects of pollutants effluent from a steam-power plant on coastal bivalves, Ocean Res 1988; 10(1): 47-56
  14. Matthiessen P and Brafield AE. The effect of dissolved zinc on the gills of the stickleback, Gasterosteus aculeatus, J Fish Biol 1973; 5: 607-613 https://doi.org/10.1111/j.1095-8649.1973.tb04494.x
  15. Mauri M, Orlando E, Negro M and Regoli F. Heavy metals in the Antarctic scallop, Adamussium colbecki, Mar Ecol Prog Ser 1990; 67: 27-33 https://doi.org/10.3354/meps067027
  16. Micallef S and Tyler PA. Effect of mercury and selenium on the gill function of Mytilus edulis, Mar Poll Bull 1990; 21(6): 288-292 https://doi.org/10.1016/0025-326X(90)90592-V
  17. Ministry of maritime affairs & fisheries. Oceanic environmental process tentative method, 2005; pp. 206-210
  18. Moore MN. Lysosomal cytochemistry in marine environmental monitoring, Histochem J 1990; 22: 187-191 https://doi.org/10.1007/BF02386003
  19. Morrison CM. Histology and cell ultrastructure of the mantle and mantle lobes of the eastern oyster, Crassostrea virginica, Gmelin: A summary atlas, Amer Malacol Bull 1993; 10(1): 1-24
  20. Otludil B, Cengiz EI, Yildirim MZ, Unver O and Unlu E. The effects of endosulfan on the great ramshorn snail, Planorbariua corneus (Gastropoda, Pulmonata): a histopathological study, Chemosphere 2004; 56: 707-716 https://doi.org/10.1016/j.chemosphere.2004.04.027
  21. Owen G. Lysosomes, peroxisomes and bivalves, Sci Prog Oxford 1972; 60: 299-318
  22. Park JJ and Lee JS. Cadmium toxicity on the survival rate and activity of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae), J Kor Fish Soc 2003; 36(5): 463-468
  23. Pekkarinen M. Scanning electron microscopy, whole-mount histology, and histochemistry of two Anodontine glochidia (Bivalvia: Unionidae), J Zool 1996; 74(11): 1964-1973
  24. Pentreath RJ. The accumulation and retention of $^{65}Zn$ and ${54}Mn$ by the plaice, Pleuronectes platessa L, J Exp Mar Biol Ecol 1973; 12: 1-18 https://doi.org/10.1016/0022-0981(73)90034-8
  25. Rainbow PS. The significance of trace metal concentration in marine invertebrates, In: Ecotoxicology of Metals in Invertebrates, Dallinger R and Rainbow PS eds. Lewis Publishers Boca Raton FL 1993; pp. 461
  26. Romeo M and Gnassia-Barelli M. Metal distribution in different tissues and in subcellular fractions of the Mediterranean clam, Ruditapes decussatus treated with cadmium, copper, or zinc, Comp Biochem Physiol 1995; 3: 457-463
  27. Skidmore JF. Respiration and osmoregulation in rainbow trout with gills damaged by zinc sulfate, J Exp Biol 1970; 52: 481-494
  28. Soh CT, Yoo IS, Park H, Kim SH, Kim JJ and Min DY. Experimental study on the effect of cadmium containing shellfish by the long term intake, Korean J Malacol 1993; 9(2): 85-93
  29. Suzuki KT, Kawahara S, Sunaga H and Shimojo N. Efflux of endogeous zinc liberated from metallothionein and alcohol dehydrogenase in the liver by replacement with cadmium, Toxicol Appl Phamacol 1990; 105: 413-421 https://doi.org/10.1016/0041-008X(90)90145-K
  30. Thanhan P, Sretarugsa P, Pokethitiyook P, Kruatrachue M and Upatham ES. Histopathological alterations in the edible snail, Babylonia areolata (Spotted Babylon), in acute and subchronic cadmium poisoning, Environ Toxicol 2004; 20: 142-149 https://doi.org/10.1002/tox.20088
  31. Viarengo A, Canesi L, Mazzucotelli A and Ponzano E. Cu, Zn and Cd content in different tissues of the Antarctic scallop, Adamussium colbecki: role of the metallothionein in heavy metal homeostasis and detoxification, Mar Ecol Prog Ser 1993; 95: 163-168 https://doi.org/10.3354/meps095163
  32. Watling HR and Watling RJ. Comparative effects of metals on the filtering rate of brown mussel, Perna perna, Bull Environ Contam Toxicol 1982; 29: 651-657 https://doi.org/10.1007/BF01606103
  33. Wicklund A, Norrgren L and Runn P. The influence of cadmium and zinc on cadmium turnover in the zebrafish, Brachydanio rerio, Arch Environ Contam Toxicol 1990; 3: 348-353
  34. Wong MH, Luk KC and Choi KY. The effects of zinc and copper salts on Cyprinus carpio and Ctenopharyngodon idellus, Acta Anatomica 1977; 99: 450-454 https://doi.org/10.1159/000144869
  35. Yoo H, Lee IT and Lee BG. Influence of Ag and Cu contaminated sediments on the bioaccumulation and chronic toxicity to the clam, Macoma balthica, Korean J Environ Biol 2002; 20(2): 136-145
  36. Yoo JS. Korean shells in color, Il Ji Sa Seoul 1988; pp. 196