동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제20권5호
  • /
  • Pages.1166-1173
  • /
  • 2006
  • /
  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

정향피 추출물의 혈관 이완효과 및 작용기전에 대한 연구

Study on the Mechanism of Vascular Relaxation Induced by Cortex Caryphylli

  • Song, Chul-Min (College of Oriental Medicine, Wonkwang University) ;
  • Shin, Sun-Ho (College of Oriental Medicine, Wonkwang University) ;
  • Jung, Hyun-Ae (College of Oriental Medicine, Wonkwang University) ;
  • Lee, Jun-Kyoung (Professional Graduate School of Orienal Medicine, Wonkwang University) ;
  • Cao, Li-Hua (Professional Graduate School of Orienal Medicine, Wonkwang University) ;
  • Kang, Dae-Gil (Professional Graduate School of Orienal Medicine, Wonkwang University) ;
  • Lee, Ho-Sup (Professional Graduate School of Orienal Medicine, Wonkwang University)
  • 발행 : 2006.10.25
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The aqueous extracts of Cortex Caryophylli (AEC) induced dose-dependent relaxation of phenylephrine-precontracted aorta, which was abolished by removal of functional endothelium. Pretreatment of the endothelium-intact aortic tissues with N$^G$_nitro-L-arginine methyl ester (L-NAME) or 1 H-[1,2,4]-oxadiazole-[4,3-${\alpha}$l-quinoxalin-1-one (ODQ) inhibited the relaxation induced by AEC. AEC-induced vascular relaxations were also markedly attenuated by addition of verapamil, diltiazem and glibenclamide, tetraethylammonium (TEA), respectively, while the relaxation effect of AEC was not blocked by indomethacin, atropine, or propranolol. Moreover, incubation of endothelium-intact aortic rings with AEC increased the production of cGMP. These results suggest that AEC dilates vascular smooth muscle via endothelium-dependent nitric oxide/cGMP signaling, which seems to be causally related with L-type Ca$^{2+}$ and K$^+$ channels.

키워드

참고문헌

  1. 全國韓醫科大學心系內科學敎室. 心系內科學, 서울, 書苑堂, p 22, 1999
  2. Levi, R. Therapies for perioperative hypertension: Pharma- codynamic consideration. Acta Anaesthesiol. Acand Suppi. 37(99):16-19, 1993
  3. Bosnjak, Z.J. Ion channels in vascular smooth muscle. Anesthesiology, 403: 120-127, 1993
  4. Rees, D.D., Palmer, R.M., Schulz, R., Hodson, H.F., Moncada, S. Characterization of three inhibitors endothelial Nitric oxide synthase in vitro and in vivo. Br. J. Pharmacol. 101: 746-752, 1990 https://doi.org/10.1111/j.1476-5381.1990.tb14151.x
  5. Vane, J.R., Anggard, E.E., Botting, R.M. Regulatory function of the vascular endothelium. New Med. 323: 27-36, 1990 https://doi.org/10.1056/NEJM199007053230106
  6. Luscher, T.F. Endothelium-derived nitric oxide: the endogenous nitrovasodilator in the human cardiovascular system. Eur. Heart J. 12 Suppl E:2-11, 1991
  7. Kuramochi, T., Chu, J., Suga, T. Gou-Teng (From Uncaria rhynchophylla Miquel)-induced endothelium-dependent and independent relaxations in the isolated rat aorta. Life Sci. 54: 2061-2069, 1994 https://doi.org/10.1016/0024-3205(94)00715-2
  8. Goto, H., Shimada, Y., Tanigawa, K., Sekiya, N., Shitani, T., Terasawa, K. Effect of Uncariae ramulus et Uncus on endothelium in spontaneously hypertensive rats. Am. J. Chin. Med. 27: 339-345, 1999 https://doi.org/10.1142/S0192415X99000380
  9. Goto, H., Sakakibara, I., Shimada, Y., Kasahara, Y., Terasawa, K. Vasodilator effect of extract prepared from Uncariae ramulus on isolated rat aorta. Am. J. Chin. Med. 28: 197-203, 2000 https://doi.org/10.1142/S0192415X00000246
  10. Kamata, K., Noguchi, M., Nagai, M. Hypotensive effects of thospermic acid B isolated from the extract of Salviae miltiorrhizae radix in the rat. Gen. Phamacol. 25: 69-73, 1994
  11. Chiou, W.F., Chang, P.C., Chou, C.J., Chen, C.F. Protein constituent contributes to the hypotensive and vasorelaxatant activities of Cordyceps sinensis. Life Sci. 66: 1369-1376, 2000 https://doi.org/10.1016/S0024-3205(00)00445-8
  12. Chen, Z.Y., Zhang, Z.S., Kwan, K.Y., Zhu, M., Ho, W.K., Huang, Y. Endothelium-dependent relaxation induced by hawthorn extract in rat mesenteric artery. Life Sci. 63: 1983-1991, 1998 https://doi.org/10.1016/S0024-3205(98)00476-7
  13. Kim. S.H., Kang, K.W., Kim, K.W., Kim, N.D. Procyanidins in Crataegus extract evoke endothelium-dependent vasorelaxation in rat aorta. Life Sci. 67: 121-131, 2000 https://doi.org/10.1016/S0024-3205(00)00608-1
  14. Kang, D.G., Sohn, E.J., Kwon, E.K., Han, J.H., Oh, H., Lee, H.S. Effects of berberine on angiotensin-converting enzyme and NO/cGMP system in vessels. Vascul. Pharmacol. 39: 281-286, 2002 https://doi.org/10.1016/S1537-1891(03)00005-3
  15. 신민교. 臨床本草學. 서울, 영림사, p 823, 1997
  16. Kim, S.Z., Kim, S.H., Park, J.K., Koh, G.Y., Cho, K.W. Presence and biological activity of c-type natriuretic peptide-dependent guanylate cyclase-coupled receptor in the penile corpus cavernosum. J. Urol. 159: 1741-1746, 1998 https://doi.org/10.1097/00005392-199805000-00104
  17. 허준. 東醫寶鑑. 서울, 대성출판사, p 106, 1984
  18. Moncada, S., Palmer, R.M., Higgs, E.A. Nitric oxide: Physiology. pathophysiology and pharmacology. Phamacol. Rev. 43: 109-142, 1993
  19. Forstermann, U., Closs, E.I., Pollock, J.S., Nakane, M., Schwarz, P., Gath, I., Kleinert, H. Nitric oxide synthase isozyme-characterization, purification, molecular cloning and funtion. Hypertension 23: 1121-1131, 1994 https://doi.org/10.1161/01.HYP.23.6.1121
  20. Moncada, S., Radomski, M.W., Palmer, R.M. Endothelium- derived relaxing factor: Identification as nitric oxide and role in the control of vascular tone and platelet funtion. Biochem. Pharmacol. 37: 2495-2501, 1990
  21. Ishii, K., Kerwin, J.F., Huang, Z.Y., Murad, F. NG-Nitro-L- argnine : a potent inhibitor of endothelium-derived relaxing factor formation. Eur. J. Pharmacol. 176(216):219-223, 1990 https://doi.org/10.1016/0014-2999(90)90531-A
  22. Furchgott, R.F., Zawadzki, J.V. The obligatory role of endothelial cells in therelaxation of arteterial smooth muscle by acetylcholine. Nature 288: 373-376, 1980 https://doi.org/10.1038/288373a0
  23. Peach, M.J., Loeb, A.L., Singer, H.A., Saye, J. Endothelium- derived vascular relaxaing factor. Hypertension 7: 94-100, 1985 https://doi.org/10.1161/01.HYP.7.3_Pt_2.I94
  24. Palmer, R.M., Ferrige, A.G., Moncada, S. Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature. 327: 542-546, 1987 https://doi.org/10.1038/327542a0
  25. Sa85, 8kuma, I.D., Stuehr, D.J., Gross, S.S., Nathan, C., Levi, R. Identification of arginine as a precursor of endothelium-derived nitric oxide. Proc Natl Acad Sci 864-8867, 1989
  26. Iyengar, R., Stuehr, D.J, Marletta, M.A. Macrophage synthesis of nitrite, nitriate and N-nitrosamones: Precursors and role of the respiratory burst. Proc Natl Acad Sci 84:6369-6379, 1987
  27. Schmidt, H.H., Nau, H., Wittfoht, W., Gerlach, J., Prescher, K.E., Klein, M.M., Niroomand, F., Bohme, E. Arginine is physiological precursor of endothelium-derived nitric oxide. Eur. J. Pharmacol. 154: 213-216, 1988 https://doi.org/10.1016/0014-2999(88)90101-X
  28. Rubanyi, G.M. Endothelium-derived relaxing and contracting factors. J. Cell. Biochem. 46: 27-36, 1991 https://doi.org/10.1002/jcb.240460106
  29. MacNaul, K.L., Hutchinson, N.I. Differential of iNOS and cNOS mRNA in human vascular smooth muscle cells and endothelial cells under normal and inflammatory conditions. Biochem. Biophys. Res. Commun. 196: 1330-1334, 1993 https://doi.org/10.1006/bbrc.1993.2398
  30. Meng, W., Ma, J., Ayata, C., Hara, H., Huang, P.L., Fishman, M.C., Moskowitz, M.A. ACh dilates pial arterioles in endothelial and neuronal NOS knockout mice by NO-dependent mechanisms. Am. J. Physiol. 271: H1145-H1150, 1996
  31. Arnold, W.P., Mittal, C.K., Katsuki, S., Murad, F. Nitric oxide activates guanylate cyclase and increase guanosine 3':5'-cyclic monophosphate levels in various tissue preparations. Proc. Natl. Acad. Sci. USA 74: 3203-3207, 1977
  32. Jaiswal, N.L., Lambrecht, G., Mutschler, E., Tache, R., Malik, K.U. Pharmacological characterization of the vascular muscarinic receptors mediating relaxation and contraction in rabbit aorta. J. Pharmacol. Exp. Ther. 258: 842-850, 1991
  33. Hutcheson, I.R., Griffith, T.M. Central role of intracellular calcium stores in acute flow- and agonist-evoked endothelial nitric oxide release. Br. J. Pharmacol. 122: 117-125, 1997 https://doi.org/10.1038/sj.bjp.0701340
  34. Sharma, N.R., Davis, M.J. Substance P-induced calcium entry in endothelial cells is secondary to depletion of intracelluar stores. Am. J. Physiol, 268: H962-H973, 1995
  35. Guerrero, M.F., Puebla, P., Carron, R., Martin, M.L., San Roman, L. Quercetin 3,7-dimethyl ether: a vasorelaxant flavonoid isolated from Croton schiedeanus Schlecht. J. Pharm. Pharmacol. 54: 1373-1378, 2002 https://doi.org/10.1211/002235702760345455
  36. Lemos, V.S., Freitas, M.R., Muller, B., Lino, Y.D., Queiroga, C.E., Cortes, S.F. Dioclein, a new nitric oxide- and endothelium-dependent vasodilator flavonoid. Eur. J. Pharmacol. 386: 41-46, 1999 https://doi.org/10.1016/S0014-2999(99)00747-5
  37. Ko, F.N., Huang, T.F., Teng, C.M. Vasodilatory action mechanisms of apigenin isolated from Apium graveolens in rat thoracic aorta. Biochim. Biophys. Acta 1115: 69-74, 1991 https://doi.org/10.1016/0304-4165(91)90013-7
  38. Mikkelsen, E.O., Thastrup, O., Christensen, S.B.. Effects of thapsigargin in isolated rat thoracic aorta. Pharmacol. Toxicol. 62: 7-11, 1988 https://doi.org/10.1111/j.1600-0773.1988.tb01835.x
  39. Delaflotte, S., Auguet, M., DeFeudis, F.V., Baranes, J., Clostre, F., Drieu, K., Braquet, P. Endothelium-dependent relaxations of rabbit isolated aorta produced by carbachol and by Ginkgo biloba extract. Biomed. Biochim. Acta 43: S212-216, 1984
  40. Van Breeman, C., Cauvin, C., Johns, A., Leijten, P., Yamamoto, H. $Ca^{2+}$ regulation of vascular smooth muscle. Fed. Proc. 45: 2746-2751, 1986
  41. Okabe, K., Kitimura, K., Kuriyama, H. Features of 4-aminopyridine sensitive outward current observed in single smooth muscle cells from the rabbit pulmonary artery. Pflugers Arch. 409: 561-568, 1987 https://doi.org/10.1007/BF00584654
  42. Nevala, R., Paukku, K., Korpela, R., Vapaatalo, H. Calcium-sensitive potassium channel inhibitors antagonize genistein- and daidzein-induced arterial relaxation in vitro. Life Sci. 69: 1407-1417, 2001 https://doi.org/10.1016/S0024-3205(01)01233-4
  43. Busse, R., Fleming, I. Endothelial dysfunction in atherosclerosis. J. Vasc. Res. 33(3):181-194, 1996 https://doi.org/10.1159/000159147
  44. Tanner, M.A., Bu, X., Steimle, J.A., Myers, P.R. The direct release of nitric oxide by gypenosides derived from the herb Gynostemma pentaphyllum. Nitric Oxide 3: 359-365, 1999 https://doi.org/10.1006/niox.1999.0245
  45. Kang D.G., Hur, T.Y., Lee, G.M., Kwon, T.O., Sohn, E.J., Lee, H.S. Effects of Cudrania tricuspidata water extract on blood pressure and renal functions in NO-dependent hypertension. Life Sci. 70: 2599-2609, 2002 https://doi.org/10.1016/S0024-3205(02)01547-3
  46. Kang, D.G, Oh, H., Cho, D.K., Kwon, E.K., Han, J.H., Lee, H.S. Effects of bulb of Fritillaria ussuriensis MAXIM on angiotensin converting enzyme and vascular release of NO/cGMP in rats. J. Ethnopharmacol. 81: 49-55, 2002 https://doi.org/10.1016/S0378-8741(02)00037-5
  47. Kang, D.G., Lee, J.K. Choi, D.H.. Sohn, E.J.. Moon, M.K.. Lee. H.S. Vascular relaxation by the methanol extract of Sorbus cortex via NO/cGMP pathway. Biol. Pharm. Bull. 28(5):860-864, 2005 https://doi.org/10.1248/bpb.28.860