Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Inhibitory Effects of Citaowan on Metastatic Cancer Growth and Agiogenesis in an Orthotopic Model of Breast Cancer

생쥐의 동소이식 유방암에서 자도환(慈桃丸)의 in vivo 전이암 성장 억제 및 혈관신생 억제 효과

  • Myung, Eu-Gene (Department of Pathology, College of Oriental medicine, Kyunghee University) ;
  • Kang, Hee (Department of Pathology, College of Oriental medicine, Kyunghee University) ;
  • Shim, Bum-Sang (Department of Pathology, College of Oriental medicine, Kyunghee University)
  • 명유진 (경희대학교 한의과대학 병리학교실) ;
  • 강희 (경희대학교 한의과대학 병리학교실) ;
  • 심범상 (경희대학교 한의과대학 병리학교실)
  • Published : 2006.12.25
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Abstract

To investigate the inhibitory effects of Citaowan (CTW) on the growth and angiogenesis of breast cancer in vivo. Orthotopic breast cancer model was established by injection of MDA-MB-231 cells into mammary fat pad of nude mice. Seven weeks after injection, CTW was orally administered at dose of 50, 100 mg/mouse every day for 40 days. Body weight, tumor volume, tumor apoptosis, microvessel density and tumor proliferation were evaluated, after the mice were sacrificed. The body weight and tumor volume were not significantly changed in CTW group compared with the control group. Tumor apoptosis, proliferation and microvessel density were significantly reduced in CTW group (100 mg/mouse) compared with the control group. These data indicate that CTW has anti-angiogenic and proapoptotic effects on breast cancer.

Keywords

References

  1. 대한병리학회. 병리학, 서울, 고문사. 1991
  2. Wilhelm, S.M., Collier, I.E., Marmer, B.L., Eisen, A.Z., Grant, G.A., Goldberg, G.I. SV40-transformed human lung fibroblasts secrete a 92-kDa type IV collagenase which is identical to that secreted by normal human macrophages. J Biol Chem, 264(29):17213-17221, 1989
  3. O'Reilly, M.S., Holmgren, L., Shing, Y., Chen, C., Rosenthal, R.A., Moses, M., et al. Angiostatin: a novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma. Cell 79(2):315-328, 1994 https://doi.org/10.1016/0092-8674(94)90200-3
  4. O'Reilly, M.S., Boehm, T., Shing, Y., Fukai, N., Vasios, G., Lane, W.S. et al. Endostatin: an endogenous inhibitor of angiogenesis and tumor growth. Cell 88(2):277-285, 1997 https://doi.org/10.1016/S0092-8674(00)81848-6
  5. Kang, D.I., Kim, S.H., Shim, B.S., Choi, S.H., Ahn, K.S. Study on the Angiogenic Inhibition Effects of Molyaksan. Korean Journal of Oriental Medical Pathology, 14(2):91-107, 2000
  6. Sohn, J.G., Kim, S.H., Seok, D.S., Shim, B.S., Choi, S.H., Ahn, K.S. Effects of Huoluoxiaolindan on Anti-metastasis. Korean Journal of Oriental Medical Pathology, 14(2):182-198, 2000
  7. 趙建成. 段鳳舞腫瘤積驗方. 合肥: 安徽科學技術出版社, 1991
  8. Kang, H.S., Kim, S.H., Shim, B.S., Choi, S.H., Ahn, K.S. Study on the Angiogenic Inhibition Effects of Kamijadowhan. Korean Journal of Oriental Medical Pathology, 4(2):168-181, 2000
  9. Wang, T.C., Park, J.H., Yu, Y.B., Shim, B.S., Ahn, K.S., Choi, S.H. Study on the effects of Jiaweicitaowan ingredients on angiogenic inhibition. Korean Journal of Oriental Medical Pathology, 14(2):155-167, 2000
  10. Wang, T.C., Park, J.H., Yu, Y.B., Shim, B.S., Choi, S.H., Ahn, K.S. Study on the effects of Jiaweicitaowan ingredients on angiogenic inhibition (II). Korean Journal of Oriental Medical Pathology, 15(2):235-240, 2001
  11. Fidler, I.J., Naito, S., Pathak, S. Orthotopic implantation is essential for the selection, growth and metastasis of human renal cell cancer in nude mice [corrected]. Cancer Metastasis Rev, 9(2):149-165, 1990 https://doi.org/10.1007/BF00046341
  12. Fu, X., Le, P., Hoffman, R.M. A metastatic orthotopic- transplant nude-mouse model of human patient breast cancer. Anticancer Res, 13(4):901-904, 1993
  13. Pulaski, B.A. Ostrand-Rosenberg S. Reduction of established spontaneous mammary carcinoma metastases following immunotherapy with major histocompatibility complex class II and B7.1 cell-based tumor vaccines. Cancer Res, 58(7):1486-1493, 1998
  14. Connolly, E.M., Harmey, J.H., O'Grady, T., Foley, D., Roche-Nagle, G., Kay, E. et al. Cyclo-oxygenase inhibition reduces tumour growth and metastasis in an orthotopic model of breast cancer. Br J Cancer, 87(2):231-237, 2002 https://doi.org/10.1038/sj.bjc.6600462
  15. 최승훈. 동의종양학. 서울, 행림출판. 1995
  16. Shim, B.S., Park, K.K., Choi, S.H. Anti-metastatic effects of fuzhengfangaitang on human fibrosarcoma cells HT1080. Am J Chin Med, 31(2):235-246, 2003 https://doi.org/10.1142/S0192415X03000953
  17. 全英秀. 加味慈桃丸의 항암 및 면역증강효과에 관한 실험적 연구. 서울: 경희대학교, 1998
  18. 최승훈, 정세영, 박건구. inventors; 암전이 억제 조성물. 대한민국patent 291630. 14 March, 2001