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Thermal Cycling Control System Design for Polymerase Chain Reaction(PCR) Machine

중합효소연쇄반응 기기의 온도 사이클링 제어시스템 설계

  • 김종해 (선문대학교 전자공학과) ;
  • 조용석 (건양대학교 전자정보공학과) ;
  • 오도창 (건양대학교 전자정보공학과)
  • Published : 2006.05.01

Abstract

In this study, a thermal control system which applied a Peltier device for the polymerase chain reaction(PCR) machine is to be designed. Here in order for it to easily follow the characteristics of the thermal cycle existing for gene amplification of the PCR sample, a PCR control board utilizing a thermal sensor, a Peltier, and a 8 bit microprocessor is made up. Especially a fuzzy type PD control algorithm is applied periodically in time response, and control system is implemented. For that matter, the characteristic data of subject system is obtained and analysed to begin with. Based on this analysed data, the proposed control algorithm is applied and an evaluation of the performance of the whole system take place through the PC.

Keywords

References

  1. R. A. Eeles and A. C. Stamps, Ploymerase Chain Reaction(PCR): The Technique and its applications, Austin: R G Landes Co., 1993
  2. M. J. McPherson, P. Quirke, and G. R. Taylor, PCR: A Practical Approach, IRL Press at Oxford University Press. Oxford, UK, 1991
  3. D. S. Yoon, Y. S. Lee, Y. Lee, H. J. Cho, S. W. Sung, K. W. Oh, J. H. Cha, and G. B. Lim, 'Precise temperature control and rapid thermal cycling in micromachined DNA polymerase chain reaction chip,' Journal of Micromechanics and Microengineering, vol. 12, pp. 813-823, 2002 https://doi.org/10.1088/0960-1317/12/6/312
  4. R. Higuchi, C. Fokler, G. Dollinger, and R. Watson, 'Kinetic PCR analysis, Real-time monitoring of DNA amplification reactions,' Bio/technology, Nature Pub. Co., vol. 11, no. 9, pp. 1026, 1993 https://doi.org/10.1038/nbt0993-1026
  5. S. Tzafestas and N. P. Papanikolopoulos, 'Incremental fuzzy expert PID control,' IEEE Trans. Industrial Electronics, vol. 37, pp. 365-371, Oct. 1990 https://doi.org/10.1109/41.103431
  6. W. Pedrycz, Fuzzy Control and Fuzzy Systems, second extended edition, Research studies press ltd., Taunton, Somerset, England, 1993