생명과학회지 (Journal of Life Science)

  • 제16권2호
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  • Pages.345-351
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  • 2006
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Streptococcus iniae의 유전학적 다양성과 RAPD fingerprint profile의 비교

Phylogenetic Diversity and Comparison of RAPD Fingerprint Profile of Streptococcus iniae

  • Jeong Yong-Uk (Faculty of marine science, Cheju National University) ;
  • Heo Moon-Soo (Faculty of marine science, Cheju National University)
  • 발행 : 2006.04.01
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초록

2004년부터 2005년까지 제주도에서 전형적인 연쇄구균증상을 나타내는 양식넙치로부터 Streptococcus sp.를 분리하였다. 생화학적 성상 조사와 multiplex PCR assay법을 통해 S. iniae 94 균주를 동정하였다. RAPD fingerprint profile을 조사해 본 결과 A, B, C-type 총 3가지의 genotype을 확인하였으며, 동일종에 대해 외국에서 보고된 선행 연구결과와 비교하였을 경우, 지리적으로 다른 유전적 다형현상을 나타내는 것으로 추정되었다. 근래에 제주도에 우점하는 genotype은 A-type이었고, RAPD fingerprint profile은 약 2,000 bp, 1,300 bp, 1,000 bp, 850 bp, 550 bp의 밴드 양상을 나타내었다. S. iniae의 serotype 동정에 유효한 것으로 보고되어진 P14 random primer는 유전적 다형현상에 의해 제주도에서 분리된 S. iniae의 serotype 동정에 적합하지 않은 것으로 판단되었다.

Streptococcus sp. were isolated from cultured flounder (Paralichthys olivaceus) having Streptococcosis during 2004 to 2005 in Jeju Island. Ninety four Streptococcus iniae strains were isolated using biochemical test and multiplex PCR assay. Three genotypes (A, B, C-type) of S. iniae were appeared in the RAPD analysis and they showed international or local genetic polymorphism. Presently, S. iniae having A-type is a dominant S. iniae genotype in Jeju and showed band patterns at about 550, 850, 1000, 1300 and 2000 base pares. In this study, the reported P14 random primer, that used to distinguish serotypes of S. iniae could not be applied to distinguish Jeju island S. iniae's genetic polymorphism.

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참고문헌

  1. Bachrach, G., A. Zlotkin, A. Hurvitz, D. L. Evans, and A. Eldar, 2001. Recovery of Streptococcus iniae from diseased fish previously vaccinated with a Streptococcus vaccine. Appl. Environ. Microbiol. 67, 3756-3758 https://doi.org/10.1128/AEM.67.8.3756-3758.2001
  2. Carmen R., B. Magarinos, S. Lopez-Romalde, A. E. Toranzo, and J. L. Romalde, 2003. Molecular Fingerprinting of Fish-Pathogenic Lactococcus garvieae Strains by Random Amplified Polymorphic DNA Analysis. J. Clin. Microbiol. 41, 751-756 https://doi.org/10.1128/JCM.41.2.751-756.2003
  3. Colorni, A., A. Diamant, A. Eldar, H. Kvitt, A. Zlotkin, 2002. Streptococcus iniae infections in Red Sea Cage-cultured and wild fishes. Dis. Aquat. Org. 49, 165-170 https://doi.org/10.3354/dao049165
  4. Eldar, A., P. F. Frelier, L. Assenta, P. W. Varner, S. Lawhon, H. Bercovier. 1995. Streptococcus shiloi, the name for an agent causing septicemic infection in fish, is a junior synonym of Streptococcus iniae. Int. J. Syst. Bacteriol. 45, 840-842 https://doi.org/10.1099/00207713-45-4-840
  5. Gilbert, D. A., N. Lehman, S. J. O. Brien and R. K. Wayne, 1990. Genetic fingerprinting reflects population differentiation in the California Channel Island fox. Nature 344, 764-767 https://doi.org/10.1038/344764a0
  6. Inglis, V., D. Colquhoun, M. D. Pearson, M. Miyata, and T. Aoki, 1996. Analysis of DNA relationships among Aeromonas species by RAPD (randomly amplified polymorphic DNA) typing. Aquaculture Int. 4, 43-53
  7. Kvitt, H., A. Colorni, 2004. Strain variation and geographic endemism in Streptococcus iniae. Dis. Aquat. Org. 61, 67-73 https://doi.org/10.3354/dao061067
  8. Mata, A. I., A. Gibello, A. Casamayor, M. M. Blanco, L. Dominguez, and J. F. Fernandez-Garayzabal, 2004. Multiplex PCR Assay for Detection of Bacterial Pathogens Associated with Warm-Water Streptococcosis in fish. Appl. Environ. Microbiol. 70, 2004
  9. Neeman, R., N. Keller, A. Barzilai, Z. Korenman, S. Sela, 1998. Prevalence of internalisation-associated gene, prtF1, among persisting group-A streptococcus strains isolated from asymptomatic carriers. Lancet 352, 1974-1977 https://doi.org/10.1016/S0140-6736(97)12452-7
  10. Ravelo, C., B. Magarinos, S. Lopez-Romalde, A. E. Toranzo, J. L. Romalde, 2003. Molecular Fingerprinting of Fish-Pathogenic Lactococcus garvieae Strains by Random Amplified Polymorphic DNA Analysis. J. Clin. Microbiol. 41(2), 751-756 https://doi.org/10.1128/JCM.41.2.751-756.2003
  11. Richard, F., J. Elliott, L. Shewmaker, and A. Reingold, 2005. Identification and Characterization of Sporadic Isolates of Streptococcus iniae Isolated from Humans Isolated from Humans. J. Clin. Microbiol. 43, 933-937 https://doi.org/10.1128/JCM.43.2.933-937.2005
  12. Romalde, J. L., B. Magarinos, C. Viller, J. L. Barja, A. E. Toranzo, 1999. Genetic analysis of turbot pathogenic Streptococcus parauberis strains by ribotyping and random amplified polymorphic DNA. FEMS Microbiol. Lett. 15, 179(2)
  13. Susanna, K. P., L. Patrick, C. Y. Woo, Herman Tse, Kit-Wah Leung, Samson S. Y. Wong, and Kwok-Yung Yuen, 2003. Invasive Streptococcus iniae Infections Outside North America. J. Clin. Microbiol. 41, 1004-1009 https://doi.org/10.1128/JCM.41.3.1004-1009.2003
  14. Warner, J. M., and J. D. Oliver, 1999. Randomly amplified polymorphic DNA analysis of clinical and environmental isolates of Vibrio vulnificus and other Vibrio species. Appli. Environ. Microbiol. 65, 1141-1144
  15. Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski, and S. V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6531-6535 https://doi.org/10.1093/nar/18.22.6531
  16. Zlotkin, A., S. Chilmonczyk., M. Eyngor, A. Hurvitz, C. Ghittino, A. Eldar, 2003. Trojan Horse Effect: Phagocyte- Mediated Streptococcus iniae Infection of Fish. Infection and Immunity 71(5), May

피인용 문헌

  1. Genotype and virulence of Streptococcus iniae isolated from diseased olive flounder Paralichthys olivaceus in Korea vol.80, pp.6, 2014, https://doi.org/10.1007/s12562-014-0804-0
  2. Immunomodulatory effect of sodium alginate enriched diet in kelp grouper Epinephelus brneus against Streptococcus iniae vol.30, pp.2, 2011, https://doi.org/10.1016/j.fsi.2010.11.023