Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antifungal Activity of Fistulosides, Steroidal Saponins, from Allium fistulosum L

대파(Allium fistulosum L)로부터 fistulosides의 분리와 분리 물질의 항진균 활성

  • Sohn Ho-Yong (Department of Food and Nutrition, Andong National University) ;
  • Kum Eun-Joo (Department of Food and Nutrition, Andong National University) ;
  • Ryu Hee-Young (Department of Food and Nutrition, Andong National University) ;
  • Jeon Su-Jin (Department of Food and Nutrition, Andong National University) ;
  • Kim Nam-Soon (Genome Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Son Kun-Ho (Department of Food and Nutrition, Andong National University)
  • 손호용 (안동대학교 식품영양학과) ;
  • 금은주 (안동대학교 식품영양학과) ;
  • 류희영 (안동대학교 식품영양학과) ;
  • 전수진 (안동대학교 식품영양학과) ;
  • 김남순 (한국생명공학연구원 기능유전체연구센터) ;
  • 손건호 (안동대학교 식품영양학과)
  • Published : 2006.04.01
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Abstract

Allium fistulosum L. (Welsh onion) is a perennial herb that is widely cultivated throughout the world, especially in China, Japan and Korea. Although, various activities were reported, little is known about antimicrobial activity of A. fistulosum L.. In this study, strong antimicrobial substances, fistuloside A, B, and C were isolated from the edible parts of A. fistulosum L. and their antimicrobial activity was evaluated with pathogenic- or food-spoilage microorganism based on disk-diffusion assay, minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) determination. Fistuloside A and fistuloside C showed strong antifungal activity and anti-Proteus activity, while fistuloside B is effective to only fungi. The fistuloside C showed a prominent antifungal activity with $3.1{\sim}6.2{\mu}g/ml$ of MIC and MFC. Our results indicated that fistuloside C has a prominent antifungal activity and support the use of A. fistulosum to treat microbial infection.

대파(Allium fistulosum L)는 백합과 다년초로서 한국, 일본 중국 등 전 세계적으로 식용으로 재배되어 왔다. 현재까지 대파의 다양한 생리활성이 보고되어 왔으나, 항균 활성에 대해서는 거의 보고되어 있지 않다. 본 연구에서는 대파 식용부위로부터 강력한 항미생물성 steroidal saponins (fistuloside A, B, 및 C)을 분리하였고, 이들의 항균력을 병원성균 및 식품부패균을 대상으로 평가하였다. Fistuloside A 및 C는 강력한 항진균 활성과 항Proteus 세균활성을 나타낸 반면, fistuloside B는 항진균 활성만 인정되었다. 특히 fistuloside C는 $3.1{\sim}6.2{\mu}g/ml$의 MIC (minimal inhibitory concentration) 및 MFC (minimal fungicidal concentration)를 나타내어 강력한 항진균 활성이 진균사멸에 의한 항균효과임을 확인하였다. 본 연구결과는 대파가 미생물 감염증 제어용으로 사용되어 온 근거를 제시하며, 또한 fistuloside C의 항진균제로서의 이용 가능성을 제시하고 있다.

Keywords

References

  1. Al-Waili, N. S. 2004. Investigating the antimicrobial activity of natural honey and its effects on the pathogenic bacterial infections of surgical wounds and conjunctiva. J. Med. Food 7, 210-222 https://doi.org/10.1089/1096620041224139
  2. Carotenuto, A., E. Fattorusso, V. Lanzotti, S. Mango, V. de Feo and C. Cicala. 1997. The flavonoids of Allium neopolitanum. Phytochemistry 44, 949-957 https://doi.org/10.1016/S0031-9422(96)00663-2
  3. Chen, J.-H., H.-I. Chen, J.-S. Wang, S.-J. Tsai and C. J. Jen. 2000. Effect of welsh onion extracts on human platelet function in vitro. Life Sci. 66, 1571-1579 https://doi.org/10.1016/S0024-3205(00)00477-X
  4. Do, J. C., K. Y. Jung and K. H. Son. 1992. Steroidal saponins from the subterranean part of Allium fistulosum. J. Natural Prod. 55, 168-173 https://doi.org/10.1021/np50080a003
  5. Fattorusso, E, V. Lanzotti, O. Taglialatela-Scafati and C. Cicala. 2001. The flavonoids of leek, Allium porrum. Phytochemistry 57, 565-569 https://doi.org/10.1016/S0031-9422(01)00039-5
  6. Grayer, R. J. and J. B. Harborne. 1994. Survey of antifungal compounds from higher plants. Phytochemistry 37, 19-42 https://doi.org/10.1016/0031-9422(94)85005-4
  7. Iwalokun, B. A., A. Ogunledun, D. O. Ogbolu, S. B. Bamiro and J. Jimi-Omojola. 2004. In vitro antimicrobial properties of aqueous garlic extract against multidrug- resistant bacteria and Candida species from Nigeria. J. Med. Food 7, 327-333 https://doi.org/10.1089/jmf.2004.7.327
  8. Jiangsu New Medical College. 1986. Zhong-yao-da-ci-dian, pp. 2316-2318, In: Zhong-yao-da-ci-dian. Shanghai Science and Technology Publisher, Shanghai
  9. Lam, Y. W., H. X. Wang and T. B. Ng. 2000. A robust cysteine-deficient chitinase-like antifungal protein from inner shoots of the edible chive Allium tuberosum. Biochem. Biophys. Res. Comm. 279, 74-80 https://doi.org/10.1006/bbrc.2000.3821
  10. Negi, P. S., C. Anandharamakrishnan and G. K. Jayaprakasha, 2003. Antibacterial activity of Aristolochia bracteata root extracts. J. Med. Food 6, 401-403 https://doi.org/10.1089/109662003772519994
  11. Phay, N., H. Higashiyama, M. Tsuji, H. Matsuura, Y. Fukushi, A. Yokota and F. Tomita. 1999. An antifungal compound from roots of welsh onion. Phytochemistry 52, 271-274 https://doi.org/10.1016/S0031-9422(99)00170-3
  12. Sang, S., A. Lao, Y. Wang, C.-K. Chin, R. T. Rosen and C.-T. Ho. 2002. Antifungal constituents from the seeds of Allium fistulosum L. J. Agric. Food Chem. 50, 6318-6321 https://doi.org/10.1021/jf025651o
  13. Seo, G.-W., J.-Y. Cho, J.-H. Kuk, J.-H. Wee, J.-H. Moon, S.-H. Kim and K.-H. Park. 2003. Identification of antioxidative substances in Allium fistulosum L. by GC-MS. Korean J. Food Sci. Technol. 35, 988-993
  14. Shogakukan. 1985. The Dictionary of Chinese drugs. pp. 1599-1600, In: The Dictionary of Chinese drugs Vol. 3. Shanghai Science and Technology Publisher and Shougakukan, Tokyo
  15. Sohn, H.-Y., K. H. Son, C.-S. Kwon, G.-S. Kwon and S. S. Kang. 2004. Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai. Phytomedicine 11, 666-672 https://doi.org/10.1016/j.phymed.2003.09.005
  16. Yamamoto, Y., S. Aoyama, N. Hamaguchi and G.-S. Rhi. 2005. Antioxidative and antihypertensive effects of welsh onion on rats fed with a high-fat high-sucrose diet. Biosci. Biotecnol. Biochem. 69, 1311-1317 https://doi.org/10.1271/bbb.69.1311
  17. Yin, M.-C. and S.-M. Tsao. 1999. Inhibitory effect of seven Allium plants upon three Aspergillus species. Int. J. Food Microbiol. 49, 49-56 https://doi.org/10.1016/S0168-1605(99)00061-6
  18. Wang, B.-S., J.-H. Chen, Y.-C. Liang and P.-D. Duh. 2005. Effects of welsh onion on oxidation of low-density lipoprotein and nitric oxide production in macrophage cell line RAW 264.7. Food Chem. 91, 147-155 https://doi.org/10.1016/j.foodchem.2004.06.009

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