생명과학회지 (Journal of Life Science)

  • 제16권2호
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  • Pages.282-288
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  • 2006
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Pleurotus eryngii 로부터 항암물질의 분리

Antitumor Sterol Isolated from the Fruiting Body of Pleurotus eryngii

  • 이영훈 (진주산업대학교 미생물공학과) ;
  • 박기훈 (경상대학교 응용생명과학부) ;
  • 이병원 (경상대학교 응용생명과학부) ;
  • 조용운 (진주산업대학교 미생물공학과) ;
  • 최영주 (신라대학교 식품영양학부) ;
  • 갈상완 (진주산업대학교 미생물공학과)
  • Lee Young-Hoon (Department of Applied Life Science, Gyeongsang National University) ;
  • Park Ki-Hun (Department of Microbiological Engineering, Chinju National University) ;
  • Lee Byong-Won (Department of Microbiological Engineering, Chinju National University) ;
  • Cho Yong-Un (Department of Applied Life Science, Gyeongsang National University) ;
  • Choi Young-Ju (Department of Food and Nutrition, Silla University) ;
  • Gal Sang-Wan (Department of Applied Life Science, Gyeongsang National University)
  • 발행 : 2006.04.01
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초록

새송이버섯으로부터 활성추적법으로 항암활성이 있는 물질인 에르고스테롤 프록사이드를 분리하였다. 이 스테롤의 구조는 분광법과 NMR법으로 확인하였으며 분자식은 $C_{28}H_{44}O_3$이었다. 폐암과 난소암에 $IC_{50}$값은 각각 $7{\mu}M$과 $14{\mu}M$이었다. DNA단편화 실험에서 이 화합물은 암세포의 chromosimal DNA 를 사닥다리모양으로 분해하였고, 세포 분열주기의 억제실험에서 G1단계를 억제함을 관찰하였다.

Activity-guided fractionations led to the isolation of antitumor compound, ergosterol peroxide ($5{\alpha},\;8{\alpha}-epideoxy-24(R)-methylcholesta-6,\;22-dien-3{\beta}-ol$) from the fruiting body of Pleuratus eryngii that was cultivated artificially. This sterol structure was established by using spectroscopic methods ($^1H\;and\;^{13}C$ nuclear magnetic resonance and high resolution mass spectra). The purified compound showed a molecular formular of $C_{28}H_{44}O_3$ displaying characteristic features of epidioxy sterols. The 50% inhibitory concentrations ($IC_{50}$) of ergosterol peroxide against human lung cancer cell line (A549) and human ovarian cell line (SK-OV3) were $7{\mu}M\;and\;14{\mu}M$, respectively. In the DNA fragmentation assay, the compound showed the programmed cell death causing the chromosomal DNA fragmentation. It reveals that ergosterol peroxide arrests G1 phase of the cell division cycle.

키워드

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피인용 문헌

  1. Anti-inflammatory and Anti-cancer Effects of Sterol-rich Fraction from Nannochloropsis oculata by using Saponification vol.47, pp.6, 2014, https://doi.org/10.5657/KFAS.2014.0770