생명과학회지 (Journal of Life Science)

  • 제16권2호
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  • Pages.253-258
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  • 2006
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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산마늘의 지역적 변이와 종다양성 연구

Population Structure and Genetic Diversity of Garlic in Korea by ISSR Marker

  • 허만규 (동의대학교 분자생물학과) ;
  • 성정숙 (농촌진흥청 인삼 약초과) ;
  • 최주수 (동의대학교 분자생물학과) ;
  • 정영기 (동아대학교 응용생물공학부) ;
  • 류은주 (한서대학교 미용학과) ;
  • 정경태 (동의대학교 응용생명과학과)
  • Huh Man-Kyu (Department of Molecular Biology, Dong-eui University) ;
  • Sung Jung-Sook (Department of Ginseng & Medical Crops, National Institute of Crop Science, RDA) ;
  • Choi Joo-Soo (Department of Molecular Biology, Dong-eui University) ;
  • Jeong Young-Kee (Department of Biotechnology, Dong-A University) ;
  • Rhu Eun-Ju (Department of Cosmetology, Hanseo University) ;
  • Chung Kyung-Tae (Department of Life Science and Biotechnology, Dong-eui University)
  • 발행 : 2006.04.01
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초록

마늘은 전 세계적으로 분포하는 다년생 초본이다. 마늘은 약리적, 경제적으로 중요한 작물이다. 야생종과 재배종의 유전관계를 ISSR 마커로 조사하였다. 또 ISSR 분석으로 이들 종의 유전적 다양도와 집단구조를 실시하였다. 한국의 세 야생 집단은 분리되어 있고 패치 분포를 보이지만 재배종에 비해 높은 유전적 다양성을 유지하고 있었다. ISS5R 마커로 야생종과 재배종의 계통관계는 잘 분리되었다. 비록 한국내 재배종 마늘이 산마늘에서 진화하였 다는 직접적 증거는 없지만 본 연구 결과 그런 가능성은 시사된다. 또한 야생종 산마늘 집단은 생식질 동정과 재배종 마늘의 진화과정에서 유익하게 쓰일 수 있다.

Garlic is a perennial herb primarily distributed throughout the world. These plants are regarded as a medically and agricultural important crop in the world. The genetic relationships between cultivated and wild species were investigated at the population levels by constructing tree based on ISSR (inter-simple sequence repeats) markers. In addition, ISSR analysis was also conducted to estimate genetic diversity and population structure of these species. Three wild garlic populations in Korea were found to have more alleles per locus (mean 1.672 vs. 1.510) higher percent polymorphic locus (67.2 vs. 51.0), and higher diversity (0.250 vs. 0.198) than three cultivated populations. The cultivated and wild species in Korea are well separated from each other at phylogenetic trees. Although there is not direct evidence that A. victorialis is an ancestor of Korean A. sativum, there is a possibility that cultivated A. sativum in Korea has evolved from wild A. victorialis in Korea. Populations of A. victorialis may be useful in germ-plasm classification and evolutionary process.

키워드

참고문헌

  1. Allnutt, T. R., A. C. Newton, A. Premoli and A. Lara. 2003. Genetic variation in the threatened South American conifer Pilgerodendron uviferum (Cuperssaceae), detected using RAPD markers. Biol. Conserv. 114, 245-253 https://doi.org/10.1016/S0006-3207(03)00044-2
  2. Barrett, S. C. H., C. G. Eckert and B. C. Husband. 1993. Evolutionary processes in aquatic plant populations. Aquat. Bot. 44, 105-145 https://doi.org/10.1016/0304-3770(93)90068-8
  3. Bowman, K. D., K. Hutcheson, E. P. Odum and L. R. Shenton. 1971. Comments on the distribution of indices of diversity. Stat. Ecol. 3, 315-359
  4. Bussell, L. D. 1999. The distribution of random amplified polymorphic DNA (RAPD) diversity among populations of Isotoma petraea (Lobeliaceae). Mol. Ecol. 8, 775-789 https://doi.org/10.1046/j.1365-294X.1999.00627.x
  5. Doebley, J. 1989. Isozymic evidence and the evolution of crop plants, pp. 46-72, In Soltis D. E. and P. S. Soltis (eds.), Isozymes in Plant Biology, Dioscorides Press, Portland
  6. Etoh, T. 1985. Studies on the sterility in garlic, Allium sativum L. Mem. Fac. Agr. Kagoshima Univ. 21, 77-132
  7. Felsenstein, J. 1993. PHYLIP (Phylogeny Inference Package) Version 3.5s. Distributed by the Author. Department of Genetics, Univ. Washington: Seattle
  8. Fisher, M. and D. Matthies. 1998. RAPD variation in relation to population size and plant performance in the rare Gentianella germanica. Am. J. Bot. 85, 811-819 https://doi.org/10.2307/2446416
  9. Fu, C., Y. Qiu. and H. Kong. 2003. RAPD analysis for genetic diversity in Changium smyrnioides (Apiaceae), an endangered plant. Bot. Bull. Acad. Sin. 44, 13-18
  10. Hamrick, J. L. and M. J. W. Godt. 1989. Allozyme diversity in plant species, pp. 304-319, In A. H. D. Brown, M. T. Clegg, A. L. Kahler and B. S. Weir (eds.), Plant Population Genetics, Breeding and Genetic Resources. Sinauer, Sunderland, MA
  11. Hoa, G., D. H. Lee, J. S. Lee and N. S. Lee. 2002. A study of taxonomical relationships among species of Korean Allium sect. sacculiferum (Liliaceae) and related species using inter-simple sequence repeat (ISSR) markers. Bot. Bull. Acad. Sin. 43, 63-68
  12. Huh, M. K. and H. W. Huh. 2001. Genetic diversity and population structure of wild lentil tare. Crop Sci. 41, 1940-1946 https://doi.org/10.2135/cropsci2001.1940
  13. Jiao, S. D. 2003. Ten Lectures of the Use of Medicinals. Paradigm Publications, Massachusetts, U.S.A
  14. Lee, M. K., Y. P. Lim and J. W. Bang. 2002. Genetic analysis of garlic (Allium sativum L.) cultivars using AFLP. Korean J. Genetics, 24, 75-81
  15. Lee, Y. N. 1997. Flora of Korea. Kyo-Hak Publishing Co, Seoul, Korea
  16. Lewontin, R. C. 1972. The apportionment of human diversity. Evol. Biol. 6, 381-398
  17. Maa ${\beta}$, H. I. and M. Klass. 1995. Infraspecific differentiation of garlic (Allium sativum L.) by isozymes and RAPD markers. Theor. Appl. Genet. 91, 89-97
  18. Moritz, C. 1995. Uses of molecular phylogenies for conservation. Philoso. Trans. Royal Soc. London, Series B. 349, 113-118 https://doi.org/10.1098/rstb.1995.0097
  19. Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA. 70, 3321-3323
  20. Nei, M. and W. H. Li. 1979. Mathematical model for studying genetical variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA. 74, 5267-5273
  21. Newton, A. C., T. Allnutt, A. C. M. Gillies, A. Lowe and R. A. Ennos. 1999. Molecular phylogeography, interspecific variation and the conservation of tree species. Trends Ecol. Evol. 14, 140-145 https://doi.org/10.1016/S0169-5347(98)01555-9
  22. Paul, S. P., F. N. Wachira, W. Powell and R. Waugh. 1997. Diversity and genetic differentiation among populations of Indian and Kenyan tea (Camellia sinensis (L.) O. Kuntze) revealed by AFLP markers, Theor. Appl. Genet. 94, 255-263 https://doi.org/10.1007/s001220050408
  23. Prokhanov, J. I. 1930. About culinary of China and Japan a critical literature view (in Russian). Bull. Appl. Bot. Genet. Plant Breed. 24, 123-183
  24. Saitou, N., and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425
  25. Yeh, F. C., R. C. Yang and T. Boyle. 1999. POPGENE Version 1.31, Microsoft Windows-based Freeware for Population Genetic Analysis. University of Alberta, Alberta