생명과학회지 (Journal of Life Science)

  • 제16권2호
  • /
  • Pages.213-218
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  • 2006
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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알로자임을 이용한 청각의 유전적 다양성과 집단구조

Genetic Diversity and Population Structure of Codium fragile (SURINGAR) HARlOT in Korea Using Allozymes

  • 이복규 (동의대학교 자연과학대학 분자생물학과) ;
  • 박소혜 (동의대학교 자연과학대학 분자생물학과) ;
  • 허윤성 (동의대학교 자연과학대학 분자생물학과) ;
  • 주무열 (동의대학교 자연과학대학 분자생물학과) ;
  • 최주수 (동의대학교 자연과학대학 분자생물학과) ;
  • 허만규 (동의대학교 자연과학대학 분자생물학과)
  • Lee Bok-Kyu (Department of Molecular Biology, Dongeui University) ;
  • Park So-Hye (Department of Molecular Biology, Dongeui University) ;
  • Heo Youn-Seong (Department of Molecular Biology, Dongeui University) ;
  • Ju Mu-Teol (Department of Molecular Biology, Dongeui University) ;
  • Choi Joo-Soo (Department of Molecular Biology, Dongeui University) ;
  • Huh Man-Kyu (Department of Molecular Biology, Dongeui University)
  • 발행 : 2006.04.01
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초록

알로자임 분석을 이용하여 청각의 유전적 다양성과 집단구조를 분석하였다. 이 종은 한국내 생태적, 경제적 중요한 자원이지만 유전적 분석이 수행되지 않았다. 전분 젤 전기영동으로 이 종의 한국내 네 집단에 대해 알로자임 변이와 유전 구조를 조사하였다. 15개 대립유전자좌위에 대해 9개 좌위(60.0%)가 적어도 한 집단에 대해 다형현상을 나타내었다. 종수준에서 유전적 다양성은 매우 높았다($H_{ES}$=0.144). 집단수준에서 유전적 다양성은 비교적 낮았다($H_{EP}$=0.128). 청각에서 전체 유전적 다양도의 87%는 집단내에 내포되어 있었다. 청각의 번식방법은 유성생식보다는 무성생식이 우세하고, 집단의 단절, 낮은 자손의 생성, 지리적 격리, 그리고 정착과정이 낮은 유전적 다양성을 설명하는 요인으로 사료된다. 조사한 청각 집단에서 세대당 이주하는 개체수는 1.69로 평가되었다. 이 값은 보통 수준의 유전자 흐름으로 해류를 통한 이동이 주된 요인으로 보인다.

The study of genetic diversity and population structure was carried out in the Codium fragile using allozyme analysis. Although this species has been regarded as a ecologically and economically important source, there is no report on population structure in Korea. Starch gel electrophoresis was used to investigate the allozyme variation and genetic structure of four Korean populations of this species. Of the 15 genetic loci surveyed, nine (60.0%) was polymorphic in at least one population. Genetic diversity was high at the species level ($H_{ES}$=0.144), and, that of the population level was relatively low ($H_{EP}$=0.128). Nearly 87% of the total genetic diversity in C. fragile was apportioned within populations. The predominant asexual reproduction, population fragmentation, low fecundity, geographic isolation and colonization process are proposed as possible factors contributing to low genetic diversity in this species. The indirect estimated of gene flow based on $G_{ST}$ was 1.69. The moderate level of gene flow in C. fragile populations is mainly caused by thallus developed from isolated utricles dispersal via sea current.

키워드

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