DOI QR코드

DOI QR Code

Short-term Toxicity Assay Based on Daphnid Feeding on the Microalga Scenedesmus subspicatus

  • Lee Sang-Ill (Department of Environmental Engineering, Chung Buk National University) ;
  • Park Jong-Ho (Department of Inland Fisheries Research Institute) ;
  • Lee Won-Ho (Department of Construction & Urban Engineering, Chung Ju National University) ;
  • Yeon Ik-Jun (Department of Environmental Engineering, Chung Ju National University) ;
  • Lee Byoung-Chan (Department of Environmental Engineering, Chung Ju National University) ;
  • Cho Kyu-Seok (Department of Inland Fisheries Research Institute) ;
  • Choi Hyun-Ill (POSCO Engineering & Construction Co., Ltd.)
  • Published : 2006.03.01

Abstract

We developed and evaluated a method of short-term acute toxicity testing based on the feeding behavior of Ceriodaphnia dubia. In prior toxicity tests, neonates of C. dubia were hatched and cultivated with the addition of yeast only for the preparation of the transparent daphnid's gut. Scenedesmus subspicatus was supplied as food after 1 to 6 h of exposure to toxicants. The effects of 1-h and 6-h exposure time on test sensitivity did not significantly differ. A comparison of the short-term l-h acute toxicity test developed in this study to the standard 48-h acute toxicity test using heavy metals, cyanide, and pentachlorophenol indicated that the 1-h test provided an acceptable sensitivity level in toxicity testing of C. dubia..

Keywords

References

  1. APHA, AWMA and WPCF. 1998. Standard Methods for the Examination of Water and Wastewater, 17th ed., Washington, American Public Health Association, Washington, D.C., 1532
  2. ASTM. 1988. Standard Practice for Conducting Acute Toxicity Tests with Fishes, Macroinvertebrates, and Amphibians. ASTM E-729-88, American Society for Testing and Materials, Philadelphia, PA., 48-69
  3. Bitton, G., K. Rhodes, B. Koopman and M. Cornejo. 1995. Short-term toxicity assay based on daphnid feeding behaviour. Wat. Environ. Res., 67, 290-293 https://doi.org/10.2175/106143095X131493
  4. Bitton, G., K. Rhodes and B. Koopman. 1996. CerioFAST, an acute toxicity test based on Ceriodaphnia dubia feeding behaviour. Environ. Toxicol. Chem., 15, 123-128 https://doi.org/10.1897/1551-5028(1996)015<0123:SCCAAT>2.3.CO;2
  5. Buikema, A.L., J.G. Geiger and D.R. Lee. 1980. Daphnia toxicity test. In: Aquatic Invertebrate Bioassays, STP 715, Buikema, A.L. and J. Cairns, eds. American Society for Testing and Materials, Philadelphia, PA, 48-69
  6. Finney. 1971. Probit Analysis. Cambridge University Press, Cambridge, Eng. Janssen, C.R. and G. Persoone. Rapid toxicity screening tests for aquatic biota. I. Methodology and experiments with Daphnia magna. Environ. Toxicol. Chem., 12, 711-717 https://doi.org/10.1897/1552-8618(1993)12[711:RTSTFA]2.0.CO;2
  7. Janssen, C.R., G. Persoone., L. Fernandez-Fuentes, M. Calleja, M. Van Steertegem and L. Brendonck. 1993. The IQ Toxicity $Test^{TM}$: A Promising Rapid Acute Mortality Test for Crustaceans, Aqua Survey, Inc., Flemington, NJ, 1-12
  8. Lee, S.I., D.G. Park and I.S. Seo. 1993. Development application of short-term toxicity bioassay technique on the inhibition of ${\beta}$-galactosidase biosynthesis. Korean Solid Waste Eng. Soc., 11, 331-338
  9. Macriorwaski, H.D. and R. Clarke. 1980. Advantages and disadvantages of using invertebrates in toxicity testing. In: Aquatic Invertebrate Bioassays, STP 715, Buikema, A.L. and J. Cairns, eds. American Society for Testing and Materials, Philadelphia, PA, 36-47
  10. Mazidji, C.N., B. Koopman and G. Bitton. 1992. Chelating resin versus ion exchange resin for heavy metal removal in toxicity fractionation. Wat. Sci. Technol., 26, 189-196 https://doi.org/10.2166/wst.1992.0399
  11. Peltier, W.H. and C.I. Weber. 1985. Methods for measuring the acute toxicity of effluents to freshwater and marine organisms. 3rd Ed., EPA/600/4-85/013, U.S. Environ. Prot. Agency, Cicinnati, Ohio, 1-120
  12. Rhodes, K.A. 1992. Rapid acute toxicity test. M.S. Thesis Florida Univ., 52-69
  13. U.S. Environmental Protection Agency. 1985a. Acute toxicity tests for freshwater invertebrates. Report EPA/540/9-85/005.U.S.0-013, U.S. Environ. Prot. Agency, Cicinnati, Ohio, 147-174
  14. U.S. Environmental Protection Agency. 1991. Methods for measuring the acute toxicity of effluents in receiving waters to freshwater and marine organisms. 4th Ed., EPA/600/4-90/027, U.S.Environ. Prot. Agency, Cicinnati, Ohio., 1-302
  15. U.S. Environmental Protection Agency. 1994. User's Guide; Probit Program Version1.5, Ecol. Monit. Res. Div., Cincinnati, Ohio, 1-154