Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Comparison of Nucleic Acid Levels, Ratio and Ecophysiological Aspects among Three Populations of the Fleshy Prawn Fenneropenaeus chinensis in Korea

  • Published : 2006.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Using biochemical methods, we determined the potential of local female shrimp populations as breeding stock to select the best adult prawns for improving larval production. As condition indexes, we selected total RNA, DNA, their ratio, and trypsin activity. The DNA content in the pleopods of each local population was similar, i.e., between $0.90{\pm}0.06\;and\;1.02{\pm}0.04(SE){\mu}g/mg$. In comparison, the RNA contents differed markedly between $2.00{\pm}0.09$ and $0.96{\pm}0.08\;{\mu}g/mg$. Therefore, the RNA/DNA (R/D) ratio in the pleopod could be used as a condition index because it represents a biochemical characteristic of the population. The mean pleopodal R/D ratio of the Goheung population was the highest at $2.52{\pm}0.19$, which indicated the best condition. Trypsin activity was influenced little by shrimp condition and more by the amount of food ingested. The gonadosomatic index (GSI) and R/D ratio in the gonads provided offsetting information about the instantaneous gonad maturity. The Goheung population had the highest instantaneous GSI, despite some spawning. Based on the condition indexes and time of gonad maturation, the Goheung shrimp population is suitable for use as breeding stock.

Keywords

References

  1. Anger, K., H. J. Hirche. 1990. Nucleic acids and growth of larvae and juvenile spidercrab, Hyas araneus. Mar. Biol. 105, 403-411 https://doi.org/10.1007/BF01316311
  2. Belchier, M., C. Clemmesen, L. Cortes, T. Doan and A. Folkvord, A. Garcia, A. Geffen, H. Hoje, A. Johannessen, E. Moksness, H. de Pontual, R. Ramirez, D. Schnack and B. Sveinsbo. 2004. Recruitment studies: manual on precision and accuracy of tools. ICES Techniques in Marine Environmental Sciences, No. 33, Copenhagen, Denmark, 1-35
  3. Brachet, J. 1961. Nucleocytoplasmic interactions in unicellular organisms. In: The Cell. Brachet. J., Mirsky, A.E. ed., Academic Press, New York. 771-841
  4. Buckley, L.J. 1984. RNA-DNA ratio: an index of larval fish growth in the sea. Mar. Biol. 80, 291-298 https://doi.org/10.1007/BF00392824
  5. Bulow, F.J., 1970. RNA-DNA ratios as indicators of recent growth rates of a fish. J. Fish. Res. Bd. Can. 27, 2343-2349 https://doi.org/10.1139/f70-262
  6. Bulow, F.J., M.E. Zeman, J.R. Winningham and W.F. Hudson. 1981. Seasonal variations in RNA-DNA ratios and indicators of feeding, reproduction, energy storage, and condition in a population of bluegill, Lepomis macrochirus Rafinesque. J. Fish Biol., 18, 237-244 https://doi.org/10.1111/j.1095-8649.1981.tb03766.x
  7. Cha, H.K., C.W. Oh, S.Y. Hong and K.Y. Park. 2002. Reproduction and population dynamics of Penaeus chinensis (Decapoda: Penaeidae) on the western coast of Korea, Yellow Sea. Fish. Res. 56, 25-36 https://doi.org/10.1016/S0165-7836(01)00310-1
  8. Chen, J.C. and C.Y. Lin. 1992. Lethal effects of ammonia on Penaeus chinensis Osbeck juveniles at different salinity levels. J. Exp. Mar. Biol. Ecol. 156, 139-148 https://doi.org/10.1016/0022-0981(92)90021-2
  9. Chen, J.C. and C.Y. Lin. 1995. Responses of oxygen consumption, ammonia-N excretion and urea-N excretion of Penaeus chinensis exposed to ambient ammonia at different salinity and pH levels. Aquaculture 136, 243-255 https://doi.org/10.1016/0044-8486(95)01060-2
  10. Chen, J.C., J.N. Lin, C.T. Chen and M.N. Lin. 1996. Survival, growth and intermoult period of juvenile Penaeus chinensis (Osbeck) reared at different combinations of salinity and temperature. J. Exp. Mar. Biol. Ecol. 204, 169-178 https://doi.org/10.1016/0022-0981(96)02589-0
  11. Chicharo, L.M.Z. and M.A. Chicharo. 1995. The RNA/DNA ratio as a useful indicator of the nutritional condition in juveniles of Ruditapes decussatus. Sci. Mar. 59 (Suppl 1), 95-101
  12. Chu, K.H., Q.C. Chen, L.M. Hung and C.K. Wong. 1995. Morphometric analysis of commercially important penaeid shrimps from the Zhujiang estuary, China. Fish. Res. 23, 89-93
  13. Clarke, A., P.G. Rodhouse, J.J. Holmes and P.L. Pascoe. 1989. Growth rate and nucleic acid ratio in cultured cuttlefish Sepia officinalis (Mollusca: Cephalopoda). J. Exp. Mar. Biol. Ecol. 133, 229-240
  14. Clemmesen, C. 1994. The effect of food availability, age or size on the RNA and DNA content in individual measured herring larvae: laboratory calibration. Mar. Biol. 118, 377-382 https://doi.org/10.1007/BF00350294
  15. Fukuda, M., H. Sako, T. Shigta adn R. Shibata. 2001. Relationship between growth and biochemical indices in laboratory reared juvenile Japanese flounder (Paralichthys olivaeus), and its application to wild fish. Mar. Biol. 138, 47-55 https://doi.org/10.1007/s002270000431
  16. Garcia, S. 1985. Reproduction, stock assessment models and population parameters in exploited penaied shrimp populations. In: Proceedings of the Second Australian National Prawn Seminar, Rothlisberg, P.C., Hill, B.J., Staples, D.J. ed., Cleveland, Australia, NSP2, 139-158
  17. Hur, S.B. and H.J. Kim. 1996. Effect of temperature and salinity on hatching rate of fertilized egg in fleshy shrimp, Penaeus chinensis. J. Kor. Fish. Soc. 29, 1-8
  18. Kim, B.A. 1973. Studies on the distribution and migration of Korean shrimp, Penaeus orientalis Kishinouye, in the Yellow Sea. Bull. Fish. Res. Dev. Agency, Busan 11, 7-23
  19. Kim, S.K., H. Rosenthal, C. Clemmesen, K. Y. Park, D.H. Kim, Y.S. Choi and H.C. Seo. 2005. Various methods to determine the gonadal development and spawning season of the purplish Washington clam, Saxidomus pupuratus (Sowerby), J. Appl. Ichthyol. 21, 101-106 https://doi.org/10.1111/j.1439-0426.2004.00636.x
  20. Lee, J.Y. 2005. The study on the reproductive physiology of the fleshy prawn, Fenneropenaeus chinensis. Ph.D. Thesis, Dong Eui University, 1-114
  21. Mustafa, S. 1977. Influence of maturation on the concentrations of RNA and DNA in the flesh of the catfish Clarius batrachus. Trans. Am. Fish. Soc., 106, 449-451 https://doi.org/10.1577/1548-8659(1977)106<449:IOMOTC>2.0.CO;2
  22. Neidhardt, F.C and B. Magasanic. 1960. Studies on the role of ribonucleic acid in the growth of bacteria. Biochem. Biophys. Acta, 42, 99-116 https://doi.org/10.1016/0006-3002(60)90757-5
  23. Nolting, M., B. Ueberschaer and H. Rosenthal. 1999. Trypsin activity and physiological aspects in larval rearing of European sea bass (Dicentrarchus labrax) using live prey and compound diets. J. Appl. Ichthyol., 15, 138-142 https://doi.org/10.1046/j.1439-0426.1999.00138.x
  24. Ueberschaer, Bern. B.H. Pedersen and K. Hjelmeland, 1992. Quantification of trypsin with radioimmunoassay in herring larvae (Clupea harengus) compared with a highly sensitive fluorescence technique to determine tryptic enzyme activity. Mar. Biol., 13, 469-473
  25. Wang, J.Q., D. Li, S. Dong, K. Wang and X. Tian. 1998. Experimental studies on polyculture in closed shrimp pond I. Intensive polyculture of Chinese shrimp (Penaeus chinensis) with tilapia hybrids. Aquaculture 163, 11-27 https://doi.org/10.1016/S0044-8486(98)00165-3
  26. Wright, D.A. and E.W. Hetzel. 1985. Use of RNA/DNA ratios as an indicator of nutritional stress in the American oyster Crassostrea virginica. Mar. Ecol. Prog. Ser., 25, 199-206 https://doi.org/10.3354/meps025199
  27. Xu, X.L., W.J. Ji, J.D. Castell and R.K. O'Dor. 1994. Influence of dietary lipid sources on fecundity, egg hatchability and fatty acid composition of Chinese prawn (Penaeus chinensis) broodstock. Aquaculture, 119, 359-370 https://doi.org/10.1016/0044-8486(94)90300-X

Cited by

  1. Relationship between White Spot Symptom and Physiological Status of Two Penaeid Shrimps vol.35, pp.4, 2017, https://doi.org/10.11626/KJEB.2017.35.4.461
  2. 산란을 위해 회유한 한국 연어(Oncorhynchus keta) 지역개체군의 생화학적 특성 vol.41, pp.3, 2006, https://doi.org/10.5657/kfas.2008.41.3.182
  3. 민들조개 (Gomphina melanaegis) 생식주기에 관한 조직.생화학적 연구 vol.42, pp.1, 2009, https://doi.org/10.5657/kfas.2009.42.1.041
  4. 한국 서해안 꽃게, Portunus trituberculatus (Miers, 1876)의 난 성숙과 산란 vol.42, pp.1, 2006, https://doi.org/10.5657/kfas.2009.42.1.048