Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Capsosiphon fulvescens Extract on Lipid Metabolism in Rats Fed High Cholesterol Diet

매생이 추출물이 고콜레스테롤 식이를 급여한 흰쥐의 지질대사에 미치는 영향

  • 이정화 (조선대학교 식품영양학과) ;
  • 이유미 (조선대학교 식품영양학과) ;
  • 이재준 (조선대학교 식품영양학과) ;
  • 이명렬 (조선대학교 식품영양학과)
  • Published : 2006.04.01
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Abstract

This study was performed to investigate the effects of Capsosiphon fulvescens (CF) extract on serum and liver lipid metabolism in rats. Male Sprague-Dawley rats were administered 1% cholesterol and 0.25% sodium cholate to induce hypercholesterolemia and were divided into six groups. Experimental groups were administered with following diets; normal diet group (NC), high cholesterol diet group (HC), normal diet and CF extract (200 mg/kg) administered group (NC-CFL), normal diet and CF extract (400 mg/kg) administered group (NC-CFH), high cholesterol diet and CF extract (200 mg/kg) adwlinistered group (HC-CFL), and high cholesterol diet and CF extract (400 mg/kg) administered group (HC-CFH). The body weight gain and feed efficiency ratio were increased by high cholesterol diet, but gradually decreased to the corresponding level of the normal diet group by administered CF extract. The serum total cholesterol concentration of normal diet group was significantly higher than those of NC-CFL group and NC-CFH group. The concentrations of serum total cholesterol LDL-cholesterol and triglyceride and the atherogenic index .were tended to decrease in the CF extract administered groups compared with the high cholesterol diet group. However, HDL-cholesterol and phospholipid concentrations in serum decreased in the high cholesterol diet group and markedly increased by the CF extract administered groups. There were no differences in the concentrations of serum triglyceride, phopholipid, LDL-cholesterol, HDL-cholesterol and free cholesterol in normal diet groups. Concentrations of total cholesterol and triglyceride in liver and kidney were also markedly decreased in the CF administered groups. Total cholesterol and triglyceride concentrations of adipose tissue in the CF administered groups were also decreased compared with the high cholesterol diet group. In addition, there were no differences in the concentrations of liver, kidney and adipose tissue total lipid, triglyceride and total cholesterol in normal diet groups. The activities of heparin-releasable lipoprotein lipase (LPL) and total-extractable LPL in adipose tissue were increased in the high cholesterol diet group compared with the CF extract administered groups, but those of the NC -CFL group and NC-CFH group were similar to the normal diet group. Taken together, it is suggested that the extract of CF have an antiatherosclerotic effect by reducing cholesterol level of serum and liver. Furthermore, the extract of CF decreased LPL activity and triglyceride concentration, resulting in less lipid storage.

매생이의 체내 지질대사 개선 효능을 구명하기 위하여 in vivo에서 콜레스테롤 식이로 유발된 고콜레스테롤 혈증 억제효능 효과를 측정하여 검토한 결과, 고콜레스테롤 식이만을 급여한 HC군은 NC군에 비하여 높은 체중증가율을 나타내었다. 매생이 에탄올 추출물 투여군들은 HC군에 비하여 감소되는 경향을 나타냈으며, 특히 매생이 에탄올 추출물 고용량 병합 투여한 HC-CFH군의 체중증가율이 HC군에 비하여 유의하게 감소되었다. 정상식이에 매생이 에탄올 추출물을 투여한 NC-CFL군과 NC-CFH군은 NC군에 비해 체중증가율이 저하되 었으나 유의 차는 없었다. 식이 섭취량은 실험군들 간에 유의차가 없었다. 혈청 중 지질함량 변화는 고콜레스테롤 식이를 급여한 HC군은 NC군에 비하여 중성지방과 총콜레스테롤 함량이 증가되었으며, 고콜레스테롤 식이와 매생이 에탄올 추출물을 병합 투여한 HC-CFL군과 HC-CFH군의 경우 NC군에 가깝게 감소되었으나 유의차가 없었다. 혈청 인지질 함량은 NC군이 HC군에 비해 유의한 증가효과를 보였으며, 고콜레스테롤 식이와 매생이 에탄올 병합투여군들의 혈청 인지질 함량은 NC군 수준으로는 증가되지 않았지만 HC군에 비해서는 증가되었다. 정상식이와 매생이 에탄올 추출물을 투여한 NC-CFL군과 NC-CFH군은 혈청중성지방 및 인지질 함량이 NC군과 비슷한 수준이었으나 혈청 총콜레스테롤 함량은 유의하게 저하되었다. 혈청 LDL-콜레스테롤과 HDL-콜레스테롤 함량은 정상식이 혹은 고콜레스테롤 식이와 매생이 에탄올 추출물을 병합 투여한 군들은 HDL-콜레스테롤 함량이 HC군에 비하여 증가하는 경향이었으나, 유리콜레스테롤 함량은 유의하게 감소하는 효과를 보였다. 정상식이와 매생이 에탄올 추출물을 투여한 NC-CFL군과 NC-CFH군의 혈청 LDL-콜레스테롤, HDL-콜레스테롤 및 유리콜레스테롤 함량은 NC군과 비슷한 경향이었다. 혈청 LDL-콜레스테롤, HDL-콜레스테롤 함량 또한 임상진단에서 순환계와 관련한 진단지수인 동맥경화지수와 심혈관 위험지수는 정상식이 혹은 고콜레스테롤 식이와 매생이 에탄올 추출물을 병합 투여한 모든 군들이 고콜레스테롤 식이를 급여한 HC군과는 달리 유의적으로 낮은 수치를 나타내었으며 NC군과는 비슷한 수준이었다. 간 및 신장 중 중성지방 함량은 고콜레스테롤 식이 급여로 증가된 HC군에 비하여 매생이 고용량 병합 투여한 HC-CFH군에서 유의한 감소를 나타냈으며, 총콜레스테롤 함량도 매생이 에탄올 병합투여로 유의적으로 감소하는 경향이었다. 지방조직의 중성지방 함량은 정상식이 혹은 고콜레스테롤 식이와 매생이 에탄올 추출물을 병합 투여한 모든 군들이 HC군에 비해 유의하게 저하되었으며, NC군에 비해서도 유의 차는 없었으나 저하되었다. 간과 신장의 총콜레스테롤 함량도 매생이 추출물 공급으로 감소하였다. 지방조직의 HR-LPL과 TE-LPL 활성 모두 NC군에 비해 HC군이 증가하였고,매생이 에탄올 추출물을 경구 투여한 모든 군들은 HC군에 비해 유의하게 감소되었으나 NC군과는 비슷한 활성을 나타내었다. 이상의 실험결과 매생이 에탄올 추출물이 고콜레스테롤식이에 의한 체내 콜레스테롤과 중성지방의 상승을 억제시키는 효과와 LPL 활성 저하효과가 있다고 사료되나 어떠한 성분의 작용기전에 의한 것인지에 대한 더욱 체계적인 연구가 요구된다.

Keywords

References

  1. Moon SJ. 1996. Nutritional problems in Korea. Korean J Nutr 29: 743-752
  2. David K, Shirley AT, Lisa ML, Larry MD, David MK. 1988. Serum and liver lipids of rats fed cocoa butter, corn oil, palm kernel oil, coconut oil and cholesterol. Nutr Res 8: 87-94
  3. Levy RI. 1981. Cholesterol, lipoprotein, apoproteins and heart disease: present status and future prospects. Clin Chem 27: 653-662
  4. Lee JM, Choi SW, Cho SH, Rhee SJ. 2003. Effect of seeds extract of Paeonia lactiflora on antioxidative system and lipid peroxidation of liver in rats fed high-cholesterol diet. Korean J Nutr 36: 793-800
  5. Yokozawa T, Nakagawa T, Kitani K. 2002. Antioxidative activity of green tea polyphenol in cholesterol-fed rats. J Agric Food Chem 50: 3549-3552 https://doi.org/10.1021/jf020029h
  6. Mckenny JA. 2001. Lipid management: tools for getting to the goal. Am J Manag Care 7: 299-306
  7. Oh IS, Kang JA, Kang JS. 2002. Gender difference in the effects of gonadectomy and hypercholesterol diet on plasma and liver cholesterol and triglyceride levels, platelet aggregation and liver tissue in sprague rats. Korean J Nutr 35: 15-23
  8. Kim SH, Park HY, Park WK. 1988. Determination and physical properties of dietary fiber in seaweed products. J Korean Soc Food Sci Nutr 17: 320-325
  9. Cho KJ, Lee YS, Ryu BH. 1990. Antitumor effect and immunology activity of seaweeds toward sarcoma-180. Bull Korean Fish Soc 23: 345-352
  10. Hurch F, Meade J, Treanor R, Whinna H. 1989. Antithrombotic activity of fucoidin with heparin cofactor II, antithrombin III and thrombin. J Biol Chem 6: 361-375
  11. Ren D, Noda H, Amano H, Nishino T, Nishizawa K. 1994. Study on antihypertensive and antilipidemic effects marine algae. Fish Sci 60: 83-88 https://doi.org/10.2331/fishsci.60.83
  12. Yu HJ. 2004. Inhibitory effect on the melanogenesis of ethanol extract of Capsosiphon fulvescens. PhD Dissertation. Wonkwang University
  13. Kang YS. 2000. A comprehensive bibliography on the fishery special commodity in Korea. Suhyepmunhwasa, Seoul. p 418-421
  14. Park JC, Choi JS, Song SH, Choi MR, Kim KY, Choi JW. 1997. Hepatoprotective effect of extracts and phenolic compound from marine algae in bromobenzene-treated rats. Korean J Pharmacogn 28: 239-246
  15. Han HA. 2002. A study of flavor on Capsosiphon fulvescens. MS Thesis. Yosu University
  16. Reeves PG, Nielson FH, Fahey GC Jr. 1993. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123: 1939- 1951 https://doi.org/10.1093/jn/123.11.1939
  17. McGowan M, Artiss J, Strandbergh D. 1983. A peroxidase- coupled method for the colorimetric determination of serum triglycerides. Clin Chem 29: 538-542
  18. Richmond W. 1976. Use of cholesterol oxidase for assay of total and free cholesterol in serum by continuous flow analysis. Clin Chem 22: 1579-1588
  19. Eng L, Noble E. 1957. The maturation of rat brain myelin. Lipids 3: 157-162 https://doi.org/10.1007/BF02531734
  20. Takayama M, Itoh S, Naguski T, Tanimizn I. 1977. A new enzymatic method for determination of serum choline-containing phospholipids. Clin Chim Acta 79: 93-98 https://doi.org/10.1016/0009-8981(77)90465-X
  21. Noma A, Nakayama K, Kota M, Okabe H. 1978. Simul-taneous determination of serum cholesterol in high and low density lipoprotein with use of heparin, $Ca^{2+}$ and an anion exchange resin. Clin Chem 24: 1504-1580
  22. Friedwald W, Levy R, Fredrickson D. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18: 499-502
  23. Folch J, Lees M, Sloane-Stanley G. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497-509
  24. Zlatkis A, Zak B. 1969. Study of a new cholesterol reagent. Anal Biochem 29: 143-148 https://doi.org/10.1016/0003-2697(69)90017-7
  25. Biggs HG, Erikson TM, Moorehead WR. 1975. A manual colorimetric assay of triglyceride in serum. Clin Chem 21: 437-441
  26. Nilsson-Ehle P, Schotz MC. 1976. A stable radioactive substrate emulsion for assay of lipoprotein lipase. J Lipid Res 17: 536-541
  27. Fried SK, Zechner R. 1989. Cachectin/tumor necrosis factor decreases human adipose tissue lipoprotein lipase mRNA levels, synthesis, and activity. J Lipid Res 30: 1917-1923
  28. Iverius PH, Brunzell JD. 1985. Human adipose tissue lipopro-tein lipase: changes with feeding and relation to postheparin plasma enzyme. Am J Physiol 249: E107-E114
  29. Miller NE. 1987. The evidence for the antiatherogenicity of high density lipoprotein in man. Lipid 13: 914-919 https://doi.org/10.1007/BF02533850
  30. Oda T, Shikate T, Natio C, Suzuki H, Kametaka T. 1970. Phospholipid fatty liver: a report of three cases with a new type of fatty liver. Jpn J Exp Med 40: 127-140
  31. Gordon T, Castelli W, Dawber T. 1981. Lipoprotein, cardiovascular disease and death, the Framingham study. Arch Inter Med 141: 1128-1135 https://doi.org/10.1001/archinte.141.9.1128
  32. Tall A. 1990. Plasma high density lipoproteins metabolism and relationship to atherogenesis. J Clin Invest 86: 379-384 https://doi.org/10.1172/JCI114722
  33. Rosenfeld L. 1989. Lipoprotein analysis. Arch Pathol Lab Med 113: 1101-1110
  34. Yun YP, Kang WS, Lee MY. 1996. The antithrombotic effects of green tea catechins. J Food Hyg Safety 11: 77- 82
  35. Kang SM, Shin JY, Hwang SJ, Hong S, Jang HE, Park MH. 2003. Effects of Saengshik supplementation on health improvement in diet-induced hypercholesterolemic rats. J Korean Soc Food Sci Nutr 32: 906-912 https://doi.org/10.3746/jkfn.2003.32.6.906
  36. Lee JH. 2006. Effect of Capsosiphon fulvescens extract on lipid metabolism of rats. MS Thesis. Chosun University
  37. Simon JA, Hodgkins ML, Browner WS, Neuhaus JA, Bernert JT, Hulley SB. 1995. Serum fatty acids and the risk of coronary heart disease. Am J Epidemiol 142: 469-476 https://doi.org/10.1093/oxfordjournals.aje.a117662
  38. Grundy SM, Denke MA. 1990. Dietary influences on serum lipids and lipoproteins. J Lipid Res 31: 1149-1172
  39. Rustan AC, Nossen JO, Christiansen EN, Drevon CA. 1988. Eicosapentaenoic acid reduces hepatic synthesis and secretion of triacylglycerol by decreasing the activity of acylcoenzyme A; 1,2-diacylglycerol acyltransferase. J Lipid Res 29: 1417-1426
  40. Willumsen N, Hexeberg S, Skorve J, Lundquist M, Berge RK. 1993. Docosahexaenoic acid shows no triglyceride-lowering effects but increases the peroxisomal fatty acid oxidation in liver of rats. J Lipid Res 34: 13-22
  41. Wong SH, Marsh JB. 1988. Inhibition of apolipoprotein secretion and phosphatidate phosphohydrolase activity by eicosapentaenic and docosahexaeonic acids in the perfused rat liver. Metabolism 37: 1177-1181 https://doi.org/10.1016/0026-0495(88)90197-7
  42. Schreiner CF, Klahr S. 1991. Diet and kidney disease: the role of dietary fatty acids. Proc Soc Exp Med 230: 1-11
  43. Kim WY, Lee SJ, Jeong HJ. 1998. Effect of different types of dietary fats on renal functions in aged rats. Korean J Nutr 31: 253-262
  44. Lee JJ, Chun CS, Kin JG, Choi BD. 2000. Effect of fasting refeeding on rat adipose tissue lipoprotein lipase activity and lipogenesis: Influence of food restriction during re-feeding. J Korean Soc Food Sci Nutr 29: 471-478
  45. O'Brien KD, Gordon D, Deeb S, Ferguson M, Chait A. 1992. Lipoprotein lipase is synthesized by macrophage-derived foam cells in human coronary atherosclerotic plaques. J Clin Invest 89: 1544-1550
  46. Semenkovich CF, Coleman T, Daugherty A. 1998. Effects of heterozygous lipoprotein lipase deficiency on diet-induced atherosclerosis in mice. J Lipid Res 39: 1141-1148

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