Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)
- Volume 38 Issue 1
- /
- Pages.38-44
- /
- 2006
- /
- 1738-3544(pISSN)
- /
- 2288-1662(eISSN)
An Easy Method of Disk Diffusion Antibiotic Susceptibility Test for Detection of Erythromycin-induced Resistance to Clindamycin in Staphylococci
포도구균의 Erythromycin 유도성 Clindamycin 내성검출을 위한 간편한 디스크 확산법의 유용성
- Joo, Sae-Ick (Department of Labolatory Medicine, Seoul National University Hospital) ;
- Lee, Hyun (Department of Labolatory Medicine, Seoul National University Hospital) ;
- Lim, Kyu-Sang (Department of Labolatory Medicine, Seoul National University Hospital) ;
- Kim, Eui-Chong (Department of Labolatory Medicine, Seoul National University Hospital)
- Published : 2006.04.30
Abstract
A simple and easy modification of AST by disk diffusion was tested for the detection of induced clindamycin resistant Staphylococci and their antimicrobial susceptibility at the same time. The incidence of inducible clindamycin resistant staphylococci in blood culture and their MIC characterization at Seoul National University Hospital was analyzed by an AST contained disk approximation test (D-zone test) and Etest, respectively. Of the total 309 staphylococcal isolates, 139 (45%) isolates presented constitutive resistance to ERY and CLI (ERY-R, CLI-R phenotype), and 59 were ERY-I/R and CLI-S phenotypes. Of the 59 isolates, 19 (32%) isolates were inducible resistant to CLI. The incidence was higher in S. aureus (66.7%) than coagulase-negative staphylococci (CNS, 26.0%). Especially, methicillin-resistant staphylococci (MRSA, 100%; MRCNS, 45.5%) presented higher inducibility than methicillin susceptible (MSSA, 50%; MSCNS, 20%). For most of the inducible clindamycin resistant staphylococci (15 of 19 isolates), their ERY MIC were high (>
Keywords
- D-zone test;
- Inducible clindamycin resistant staphylococci;
- Incidence of inducible $MLS_B$;
- Methicillin resistant staphylococci