한국식품저장유통학회지 (Food Science and Preservation)

  • 제13권2호
  • /
  • Pages.246-251
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  • 2006
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  • 3022-5477(pISSN)
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  • 3022-5485(eISSN)
한국식품저장유통학회 (The Korean Society of Food Preservation)
권호 표지

감마선 조사를 이용한 유기질 퇴비의 병원성 미생물 저감화

Reduction of Pathogenic Bacteria in Organic Fertilizer using Gamma-Irradiation

  • 윤혜정 (한국원자력연구소 방사선연구원) ;
  • 임상용 (한국원자력연구소 방사선연구원) ;
  • 송현파 (한국원자력연구소 방사선연구원) ;
  • 김병근 (한국원자력연구소 방사선연구원) ;
  • 정진우 (한국원자력연구소 방사선연구원) ;
  • 김동호 (한국원자력연구소 방사선연구원)
  • Yun Hye-Jeong (Korea Atomic Energy Research Institute, Advanced Radiation Technology Institute) ;
  • Lim Sang-Yong (Korea Atomic Energy Research Institute, Advanced Radiation Technology Institute) ;
  • Song Hyun-Pa (Korea Atomic Energy Research Institute, Advanced Radiation Technology Institute) ;
  • Kim Byeong-Keun (Korea Atomic Energy Research Institute, Advanced Radiation Technology Institute) ;
  • Chung Jin-Woo (Korea Atomic Energy Research Institute, Advanced Radiation Technology Institute) ;
  • Kim Dong-Ho (Korea Atomic Energy Research Institute, Advanced Radiation Technology Institute)
  • 발행 : 2006.04.01
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초록

시판 유기질 퇴비(우분, 돈분, 계분 및 혼합분) 16종의 총 호기성 세균수, 대장균 및 대장균군 그리고 Salmonella에 대한 미생물 오염도를 파악하고, 미생물 오염도 저감화 및 위생성 향상을 위해 감마선을 조사한 후 미생물 사멸도를 측정하였다. 총 호기성 세균수는 우분, 돈분, 계분 및 혼합분의 경우 $10^4{\sim}10^\;CFU/g$으로 유의적인 차이를 나타내지 않았다. 대장균군은 16종의 시료 중 12종의 시료에서 $10^1{\sim}10^3\;CFU/g$의 분포를 나타내었고, 대장균은 3종의 시료에서 $10^1{\sim}10^2\;CFU/g$의 분포를 나타내었다. 또한 SS agar plate 분리 enteric group 미생물은 계분제품 중 1종에서만 검출되었다. 한편 총 호기성세균은 10 kGy 감마선 조사에 의해서 평균 4 log cycle 감소하였으며, 대장균군은 5 kGy 감마선 조사시 완전살균 수준의 감소효과를 나타내었다. E. coli와 SS agar plate 분리 enteric group은 3 kGy 감마선 조사에 의해 완전 사멸되었다. 유기질 퇴비 분포 미생물의 D값은 SS agar plate 분리 enteric group은 0.40 kGy, E. coli는 0.39 kGy의 범위를 나타내었다. 시판 유기질 퇴비는 3 kGy 수준에서 축분뇨발효 비료액의 병원성 미생물 공정규정을 충족할수 있으며, 위생화를 위해서는 5 kGy 수준으로 설정하는 것이 바람직할 것으로 사료된다.

Organic compost is a useful fertilizer for organic farming. However, it poses a microbiological hazard to the farm produce because most of the compose are originated from excrementitious matters of domestic animals. Irradiation was performed to improve microbiological safety of organic compost and the effectiveness of gamma-irradiation for inactivating Salmonella typhimurium and E. coli was investigated. Total aerobic bacteria and coliform bacteria in 16 produce or commercial compose were ranged from $10^5\;to\;10^7\;CFU/mL\;and\;0\;to\;10^3\;CFU/mL$, respectively. All coliform bacteria in the composts were eliminated by irradiation at 5 kGy, while about $10^2\;CFU/mL$ of total aerobic bacteria survived up to 10 kGy of irradiation. In the inoculation test the test organisms (inoculated at $10^7\;CFU/mL$) were eliminated by irradiation at 3 kGy. $D_{10}$ values of inoculated Salmonella typhimurium and E. coli in the compost were $0.4{\pm}0.05\;and\;0.39{\pm}0.03kGy$. It was considered that $3{\sim}5kGy$ of gamma irradiation was effective for radicidation (radiation sterilization of pathogenic microbes) of organic fertilizer.

키워드

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