Journal of Microbiology

  • 제44권2호
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  • Pages.226-232
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  • 2006
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Swarming Differentiation of Vibrio vulnificus Downregulates the Expression of the vvhBA Hemolysin Gene via the LuxS Quorum-Sensing System

  • Kim Moon-Young (Research Center for Resistant Cells, Chosun University Medical School) ;
  • Park Ra-Young (Research Center for Resistant Cells, Chosun University Medical School) ;
  • Choi Mi-Hwa (Research Center for Resistant Cells, Chosun University Medical School) ;
  • Sun Hui-Yu (Research Center for Resistant Cells, Chosun University Medical School) ;
  • Kim Choon-Mee (Research Center for Resistant Cells, Chosun University Medical School) ;
  • Kim Soo-Young (Clinical Vaccine R&D Center, National Research Laboratory of Molecular Microbial Pathogenesis, Research Institute of Vibrio Infection and Genome Research Center for Enteropathogenic Bacteria, and Department of Microbiology, Chonnam National University Medical School) ;
  • Rhee Joon-Haeng (Clinical Vaccine R&D Center, National Research Laboratory of Molecular Microbial Pathogenesis, Research Institute of Vibrio Infection and Genome Research Center for Enteropathogenic Bacteria, and Department of Microbiology, Chonnam National University Medical School) ;
  • Shin Sung-Heui (Research Center for Resistant Cells, Chosun University Medical School)
  • 발행 : 2006.04.01
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초록

Swarming has proven to be a good in vitro model for bacterial surface adherence and colonization, and the swarming differentiation of a bacterium has been shown to be coupled with changes in the expression of virulence factors associated with its invasiveness, particularly in the early stages of infection. In this study, we attempted to determine whether the expression of vvhA, which encodes for hemolysin/cytolysin (VvhA), is either upregulated or downregulated during the swarming differentiation of V. vulnificus. The insertional inactivation of vvhA itself exerted no detectable effect on the expression of V. vulnificus swarming motility. However, in our lacZ-fused vvhA transcriptional reporter assay, vvhA expression decreased in swarming V. vulnificus as compared to non-swarming or planktonic V. vulnificus. The reduced expression of vvhA in swarming V. vulnificus increased as a result of the deletional inactivation of luxS, a gene associated with quorum sensing. These results show that vvhA expression in swarming V. vulnificus is downregulated via the activity of the LuxS quorum-sensing system, suggesting that VvhA performs no essential role in the invasiveness of V. vulnificus via the adherence to and colonization on the body surfaces required in the early stages of the infection. However, VvhA may playa significant role in the pathophysiological deterioration occurring after swarming V. vulnificus is differentiated into planktonic V. vulnificus.

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참고문헌

  1. Allison, C., H.C. Lai, and C. Hughes. 1992. Co-ordinate expression of virulence genes during swarm-cell differentiation and population migration of Proteus mirabilis. Mol. Microbiol. 6, 1583-1591 https://doi.org/10.1111/j.1365-2958.1992.tb00883.x
  2. Connelly, M.B., G.M. Young, and A. Sloma. 2004. Extracellular proteolytic activity plays a central role in swarming motility in Bacillus subtilis. J. Bacteriol. 186, 4159-4167 https://doi.org/10.1128/JB.186.13.4159-4167.2004
  3. Fan, J.J., C.P. Shao, Y.C. Ho, C.K. Yu, and L.I. Hor. 2001. Isolation and characterization of a Vibrio vulnificus mutant deficient in both extracellular metalloprotease and cytolysin. Infect. Immun. 69, 5943-5948 https://doi.org/10.1128/IAI.69.9.5943-5948.2001
  4. Fraser, G.M. and C. Hughes. 1999. Swarming motility. Curr. Opin. Microbiol. 2, 630-635 https://doi.org/10.1016/S1369-5274(99)00033-8
  5. Fraser, G.M., L. Claret, R. Furness, S. Gupta, and C. Hughes. 2002. Swarming-coupled expression of the Proteus mirabilis hpmBA hemolysin operon, Microbiology 148, 2191-2201 https://doi.org/10.1099/00221287-148-7-2191
  6. Gulig, P.A., K.L. Bourdage, and A.M. Starks. 2005. Molecular pathogenesis of Vibrio vulnificus. J. Microbiol. 43, 118-131
  7. Harshey, R.M. 1994. Bees aren't the only ones: swarming in Gram-negative bacteria. Mol. Microbiol. 13, 389-394 https://doi.org/10.1111/j.1365-2958.1994.tb00433.x
  8. Hulsmann, A., T.M. Rosche, I.S. Kong, H.M. Hassen, D.M. Beam, and J.D. Oliver. 2003. RpoS-dependent stress response and exoenzyme production in Vibrio vulnificus. Appl. Environ. Microbiol. 69, 6114-6120 https://doi.org/10.1128/AEM.69.10.6114-6120.2003
  9. Kim, M.Y., R.Y. Park, Y.H. Bai, Y.Y. Chung, C.M. Kim, S.Y. Kim, J.H. Rhee, and S. H. Shin. 2006. X-gal inhibits the swarming of Vibrio species. J. Microbiol. Methods, in press
  10. Kim, S.Y., S.E. Lee, Y.R. Kim, C.M. Kim, P.Y. Ryu, H.E. Choy, S.S. Chung, and J.H. Rhee. 2003. Regulation of Vibrio vulnificus virulence by the LuxS quorum-sensing system. Mol. Microbiol. 48, 1647-1664 https://doi.org/10.1046/j.1365-2958.2003.03536.x
  11. Kim, Y.R. and J.H. Rhee. 2003. Flagellar basal body flg operon as a virulence determinant of Vibrio vulnificus. Biochem. Biophys. Res. Comm. 304, 405-410 https://doi.org/10.1016/S0006-291X(03)00613-2
  12. Kim, Y.R., S.Y. Kim, C.M. Kim, S.E. Lee, and J.H. Rhee. 2005. Essential role of an adenylate cyclase in regulating Vibrio vulnificus virulence. FEMS Microbiol. Lett. 243, 497-503 https://doi.org/10.1016/j.femsle.2005.01.016
  13. Lee, S.E., P.Y. Ryu, S.Y. Kim, Y.R. Kim, J.T. Koh, O.J. Kim, S.S. Chung, H.E. Choy, and J.H. Rhee. 2004. Production of Vibrio vulnificus hemolysin in vivo and its pathogenic significance. Biochem. Biophys. Res. Comm. 324, 86-91 https://doi.org/10.1016/j.bbrc.2004.09.020
  14. Miller, J.H. 1992. A short course in bacterial genetics; A laboratory manual and handbook for Escherichia coli and related bacteria. Cold Spring Harbor Laboratory Press, New York
  15. Shin, S.H., H.Y. Sun, M.H. Choi, R.Y. Park, Y.H. Bai, C.M. Kim, S.Y. Kim, Y.R. Kim, S.E. Lee, and J.H. Rhee. 2005. Inactivation of Vibrio vulnificus hemolysin by oligomerization but not proteolysis. Biol. Pharm. Bull. 28, 1294-1297 https://doi.org/10.1248/bpb.28.1294
  16. Strom, M.S. and R.N. Parangpye. 2000. Epidemiology and pathogenesis of Vibrio vulnificus. Microbes Infect. 2, 177- 188 https://doi.org/10.1016/S1286-4579(00)00270-7
  17. Walker, K.E., S. Moghaddame-Jafar, C.V. Lockatell, D. Johnson, and R. Belas. 1999. ZapA, the IgA-degrading metalloprotease of Proteus mirabilis, is a virulence factor expressed specifically in swarmer cells. Mol. Microbiol. 32, 825-836 https://doi.org/10.1046/j.1365-2958.1999.01401.x
  18. Wang, Q., J.G. Frye, M. McClelland, and R.M. Harshey. 2004. Gene expression patterns during swarming in Salmonella typhimurium: genes specific to surface growth and putative new motility and pathogenicity genes. Mol. Microbiol. 52, 169-187 https://doi.org/10.1111/j.1365-2958.2003.03977.x
  19. Wright, A.C. and J.G. Morris Jr. 1991. The extracellular cytolysin of Vibrio vulnificus: Inactivation and relationship to virulence in mice. Infect. Immun. 59, 192-197
  20. Wright, A.C., L.M. Simpson, J.D. Oliver, and J.G. Morris Jr. 1990. Phenotypic evaluation of acapsular transposon mutants of Vibrio vulnificus. Infect. Immun. 58, 1767-1773