Microarray-Mediated Transcriptome Analysis of the Tributyltin (TBT)-Resistant Bacterium Pseudomonas aeruginosa 25W in the Presence of TBT

  • Dubey Santosh K. (Center for Marine Environmental Studies (CMES), Ehime University, Department of Microbiology, Goa University) ;
  • Tokashiki Tsutomu (Center for Marine Environmental Studies (CMES), Ehime University) ;
  • Suzuki Satoru (Center for Marine Environmental Studies (CMES), Ehime University)
  • 발행 : 2006.04.01

초록

The tributyltin (TBT)-resistant bacterium, Pseudomonas aeruginosa 25W, which was isolated in seawater from the Arabian Sea, was subjected to transcriptome analysis in the presence of high concentrations of TBT. Only slight effects were observed at TBT concentration of $50{\mu}M$, but exposure to $500{\mu}M$ resulted in the upregulation of 6 genes and the downregulation of 75. Among the 75 downregulated genes, 53% (40 out of 75) were of hypothetical function, followed by 14 transcriptional regulation- and translation-associated genes. The results of this study indicated that although the 25W strain was highly resistant to TBT, high concentrations of TBT result in toxic effect on the transcriptional and translational levels. The target genes likely belong to a specific category of transcription- and translation-associated genes rather than to other gene categories.

키워드

참고문헌

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