Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Genetic Analysis of a Structural Motif Within the Conserved 530 Stem-Loop of Escherichia coli 16S rRNA

  • Szatkiewicz Jin P. (Department of Biological Sciences, Wayne State University) ;
  • Cho Hyun-Dae (Department of Biological Sciences, Wayne State University) ;
  • Ryou Sang-Mi (Department of Life Science, Chung-Ang University) ;
  • Kim Jong-Myung (Department of Life Science, Chung-Ang University) ;
  • Cunningham Philip R. (Department of Biological Sciences, Wayne State University) ;
  • Lee Kang-Seok (Department of Life Science, Chung-Ang University)
  • Published : 2006.04.01
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Abstract

The 530 stem-loop is a 46 nucleotide stem-loop structure found in all small-subunit ribosomal RNAs. Phylogenetic and mutational studies by others suggest the requirement for Watson-Crick interactions between the nucleotides 505-507 and 524-526 (530 pseudoknot), which are highly conserved. To examine the nature and functional significance of these interactions, a random mutagenesis experiment was conducted in which the nucleotides in the proposed pseudoknot were simultaneously mutated and functional mutants were selected and analyzed. Genetic analysis revealed that the particular nucleotide present at each position except 524 was not exclusively critical to the selection of functional mutants. It also indicated that basepairing interactions between the positions 505-507 and 524-526 were required for ribosomal function, and much weaker base-pairing interactions than those of the wild-type also allowed high ribosomal function. Our results support the hypothesis that the 530 pseudoknot structure may undergo a 'conformational switch' between folded and unfolded states during certain stages of the protein synthesis process by interacting with other ligands present in its environment.

Keywords

References

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