약학회지 (YAKHAK HOEJI)

  • 제50권2호
  • /
  • Pages.99-104
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  • 2006
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  • 0377-9556(pISSN)
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  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

강활 에틸아세테이트 분획에 의한 PG 분해, iNOS, $PGE_2$, 활성 억제 및 진통효과

Analgesic Activity and Inhibitory Effect of PG Degradation, iNOS and $PGE_2$ by Ethyl Acetate Fraction of Angelica Koreana Radix

  • 김시나 ((주) KMSI 부설 한국의과학연구소) ;
  • 이현지 ((주) KMSI 부설 한국의과학연구소) ;
  • 이은정 ((주) KMSI 부설 한국의과학연구소) ;
  • 남경숙 ((주) KMSI 부설 한국의과학연구소) ;
  • 김희석 ((주) KMSI 부설 한국의과학연구소) ;
  • 황성완 ((주) KMSI 부설 한국의과학연구소) ;
  • 황성연 ((주) KMSI 부설 한국의과학연구소)
  • 발행 : 2006.04.01
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초록

Prostaglandins biosynthesis and nitric oxide production have been implicated in the process of inflammation. In this study we investigated on the effects of ethyl acetate extract of Angelica Koreana Radix (EAKR) on the activities of prostaglandin $E_2\;(PGE_2)$, proteoglycan (PG) degradation and nitric oxide synthase (NO) in inflammation cytokines-activated rabbit articular chondrocytes. EAKR exhibited inhibitory activities on NO production and $PGE_2$ production as $73.08\%\;and\;89.49\%$, respectively at $20{\mu}g/ml$ and inhibited the degradation of PG in a concentration-dependent manner Zelatin zymography analysis demonstrated that EAKR significantly inhibited MMP-2, 9 expression in chondrocytes. In vivo, EAKR was shown to have inhibitory effects on acetic acid-induced pain. This study suggests that modulation of $PGE_2$, NO, PG degradation and MMP-2, 9 by EARK may be important in the prevention of inflammation and osteroarthritis.

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