Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Naturally Occurring Biflavonoid, Ochanflavone, Inhibits Cyclooxygenases-2 and 5-Lipoxygenase in Mouse Bone Marrow-derived Mast Cells

  • Son Min-Jung (Skeletal Diseases Genome Research Center, Kyungpook National University) ;
  • Moon Tae-Chul (College of Pharmacy, Yeungnam University) ;
  • Lee Eun-Kyung (College of Pharmacy, Yeungnam University) ;
  • Son Kun-Ho (Department of Food and Nutrition, Andong National University) ;
  • Kim Hyun-Pyo (College of Pharmacy, Kangwon National University) ;
  • Kang Sam-Sik (Natural Products Research Institute, Seoul National University) ;
  • Son Jong-Keun (College of Pharmacy, Yeungnam University) ;
  • Lee Seung-Ho (College of Pharmacy, Yeungnam University) ;
  • Chang Hyeun-Wook (College of Pharmacy, Yeungnam University)
  • Published : 2006.04.01
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Abstract

Ochnaflavone is a medicinal herbal product isolated from Lonicera japonica that inhibits cyclooxygenase-2 (COX-2) dependent phases of prostaglandin $D_2(PGD_2)$ generation in bone marrow-derived mast cells (BMMC) in a concentration-dependent manner with $IC_{50}$ values of $0.6{\mu}M$. Western blotting probed with specific anti-COX-2 antibodies showed that the decrease in quantity of the $PGD_2$ product was accompanied by a decrease in the COX-2 protein level. In addition, this compound consistently inhibited the production of leukotriene $C_4 (LTC_4)$ in a dose dependent manner, with an $IC_{50}$ value of $6.56 {\mu}M$. These results demonstrate that ochnaflavone has a dual cyclooxygenase-2/5-lipoxygenase inhibitory activity. Furthermore, this compound strongly inhibited degranulation reaction in a dose dependent manner, with an $IC_{50}$ value of $3.01{\mu}M$. Therefore, this compound might provide a basis for novel anti-inflammatory drugs.

Keywords

References

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