Environmental Mutagens and Carcinogens (한국환경성돌연변이발암원학회지)

Korean Environmental Mutagen Society (한국환경성돌연변이발암원학회)
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Purification of a Antimicrobial Peptide from the Marine Mussel, Mytilus coruscus

홍합 [Mytilus coruscus]으로부터 항균활성 펩타이드의 정제

  • Kim In-Hae (Department of Pharmaceutical Engineering, College of Engineering, Silla University) ;
  • Kim Jin-Wook (Department of Pharmaceutical Engineering, College of Engineering, Silla University) ;
  • Lee Jae-Hwa (Department of Pharmaceutical Engineering, College of Engineering, Silla University)
  • 김인혜 (신라대학교 공과대학 제약공학과) ;
  • 김진욱 (신라대학교 공과대학 제약공학과) ;
  • 이재화 (신라대학교 공과대학 제약공학과)
  • Published : 2006.03.01
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Abstract

Antimicrobial peptides (AMPs) play an important role in this response by rapidly killing invading microorganisms. In this study antimicrobial peptide has been isolated from acidified whole body extract of a bivalve mollusk, the marine mussel (Mytilus coruscus). This peptide purified to homogeneity by gel-filtration and reversed-phase high performance liquid chromatography. The molecular weight was 1464.92 Da, determined by MALDI-TOF Mass spectrometry. In addition to growth inhibition of Escherichia coli D31.

흥합 (Mytilus coruscus) 추출물 RM 60을 사용하여 E. coli D31을 대상으로 항균활성 펩타이드를 분리 정제하였다. 정제한 펩타이드는 MALDI-TOF Mass spectrometry를 사용하여 분자량을 측정한 결과, 1464.92 Da로 나타났으며 Edman 분해법을 이용한 peptide sequencer를 사용하여 일차구조를 분석 중에 있다.

Keywords

References

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