Clean Technology (청정기술)
- Volume 12 Issue 2
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- Pages.53-61
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- 2006
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- 1598-9712(pISSN)
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- 2288-0690(eISSN)
The Production of Protein-loaded Poly(lactide-co-glycolide) Microparticles using Supercritical Carbon Dioxide
초임계 PGSS 법을 이용한 Poly(lactide-co-glycolide)와 단백질의 마이크로복합체 제조에 관한 연구
- Song, Eun-Seok (School of Chemical and Biological Engineering & Institute of Chemical Processes, Seoul National University) ;
- Jung, Heon-Seop (Clean Technology Research Center, Korea Institute of Science and Technology) ;
- Lee, Hanho (School of Chemical and Biological Engineering & Institute of Chemical Processes, Seoul National University) ;
- Kim, Jae-Duck (Clean Technology Research Center, Korea Institute of Science and Technology) ;
- Kim, Hwayong (School of Chemical and Biological Engineering & Institute of Chemical Processes, Seoul National University) ;
- Lee, Youn-Woo (School of Chemical and Biological Engineering & Institute of Chemical Processes, Seoul National University)
- 송은석 (서울대학교 화학생물공학부) ;
- 정헌섭 (한국과학기술연구원 청정기술연구센터) ;
- 이한호 (서울대학교 화학생물공학부) ;
- 김재덕 (한국과학기술연구원 청정기술연구센터) ;
- 김화용 (서울대학교 화학생물공학부) ;
- 이윤우 (서울대학교 화학생물공학부)
- Received : 2006.01.09
- Accepted : 2006.06.20
- Published : 2006.06.30
Abstract
A PGSS (Particles from Gas Saturated Solutions) process designed to generate nano-particles using supercritical fluids has been conducted for the fabrication of Poly(lactide-co-glycolide) (PLGA) microparticles that encapsulate a protein drug. It is demonstrated that the polymer and the dry powder of a protein can be mixed under supercritical carbon dioxide conditions and that the protein component retains its biological activity. In this experiment, the mixture of polymer which is plasticized and dry powder protein was sprayed to form solid polymer that encapsulate the protein. It is found that supercritical fluid process give fine tuning of particle size and particle size distribution by simple manipulations of the process parameters. Porous particles were formed with irregular shape. Protein encapsulated in the polymer was found to have enzymatic activity without significant loss of its initial value.
생분해성 고분자인 Poly(lactide-co-glycolide) (PLGA)와 약물 단백질로 약물 중합체를 만들기 위하여 초임계 나노 입자 제조 공정중 하나인 PGSS법을 이용하여 연구를 수행하였다. 초임계 이산화탄소에 의해서 고분자 PLGA의 유리전이온도(
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