Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Periodontal tissue engineering by hPDLF seeding on scaffold

Scaffold상에 식립한 사람치주인대섬유모세포를 통한 치주조직공학

  • Kim, Seong Sin (Department of Periodontology, College of Dentistry, Chosun University) ;
  • Kim, Byung-Ock (Department of Oral Histology, Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • Park, Joo-Cheol (Department of Oral Histology, Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • Jang, Hyun-Seon (Department of Oral Histology, Oral Biology Research Institute, College of Dentistry, Chosun University)
  • 김성신 (조선대학교 치과대학 치주과학 교실) ;
  • 김병옥 (조선대학교 치과대학 치주과학 교실, 구강생물학연구소) ;
  • 박주철 (조선대학교 치과대학 구강조직학 교실, 구강생물학연구소) ;
  • 장현선 (조선대학교 치과대학 치주과학 교실, 구강생물학연구소)
  • Published : 2006.09.30
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Abstract

Human periodontal ligament fibroblasts (hPDLF) are very important for curing the periodontal tissue because they can be differentiated into various cells. A tissue engineering approach using a cell-scaffold is essential for comprehending today's periodontal tissue regeneration procedure. This study examined the possibility of using an acellular dermal matrix as a scaffold for human periodontalligament fibroblast (hPDLF). The hPDLF was isolated from the middle third of the root of periodontally healthy teeth extracted for orthodontic reasons. The cells were cultured in a medium containing Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum at $37^{\circ}C$ in humidified air with 5% $CO_2$. The acellular dermal matrix(ADM) was provided by the US tissue banks(USA). Second passage cells were used in this study. The hPDLF cells were cultured with the acellular dermal matrix for 2 days, and the dermal matrix cultured by the hPDLF was transferred to a new petri dish and used as the experimental group. The control group was cultured without the acellular dermal matrix, The control and experimental cells were cultured for six weeks. The hPDLF cultured on the acellular dermal matrix was observed by Transmission Electron microscopy (TEM). Electron micrography shows that the hPDLF was proliferated on the acellular dermal matrix. This study suggests that the acellular dermal matrix can be used as a scaffold for hPDLF.

Keywords

References

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