Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Effects of Irradiated Frozen Allogenic Bone and Musculoskeletal Transplant Foundation on Bone Formation in Human Fetal Osteoblasts

사람 태아 골모 세포에 대한 냉동 동종골과 근골격이식재의 골형성 유도에 관한 효과

  • Yoon, Ho-Sang (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Pi, Sung-Hee (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Yun, Hyeong-Geun (Department of Periodontology, School of Dentistry, Wonkwang University)
  • 윤호상 (원광대학교 치과대학 치주과학교실) ;
  • 피성희 (원광대학교 치과대학 치주과학교실) ;
  • 윤형근 (원광대학교 치과대학 치주과학교실)
  • Published : 2006.06.30
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Abstract

The purpose of this study was to investigate the effects of ICB(Irradiated frozen allogenic bone, Rocky Mountain Tissue Bank, USA) and MTF(Decalcified freeze-dried bone allograft, Musculoskeletal Transplant Foundation, USA) on the cell proliferation and differentiation of human fetal osteoblasts. Human fetal osteoblasts (hFOB1) were cultured with $10\;ng/m{\ell}$of ICB and MTF. The negatvie control group was cultured with DMSO and positive control group was cultured with BMF ($2\;ng/m{\ell}$). MIT was performed to examine the viability of the cell, and alkaline phosphatase activity was analyzed to examine the mineralization. Calcium accumulation was also evaluated. ICB and MTF did not increase the rate of the cellular proliferation of hFOB1s while they enhanced ALP and calcium accumulation. The expression of osteocalcin (OC) and bone silaloprotein (BSP) increased in hFOB1 treated with ICB and MTF ($10\;ng/m{\ell}$). These results suggest that ICB and MTF stimulate osteoblastic activity of the hFOBl.

Keywords

References

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