Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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The Effect of Chlorhexidine on the formation of bone nodules by Periodontal ligament Cells in Vitro

사람치주인대섬유모세포에 의한 골결절 형성시 Chlorhexidine의 효과

  • Choi, Hui-Jun (Department of Periodontology, College of Dentistry, Chosun University) ;
  • Ji, Suk (Department of Periodontology, College of Dentistry, Chosun University) ;
  • Kook, Joong-Ki (Department of Oral Biochemistry, College of Dentistry, Oral Biology Research Institute, Chosun University) ;
  • Jang, Hyun-Seon (Department of Periodontology, College of Dentistry, Oral Biology Research Institute, Chosun University) ;
  • Park, Joo-Cheol (Department of Oral Histology, College of Dentistry, Oral Biology Research Institute, Chosun University) ;
  • Kim, Heung-Joong (Department of Oral Anatomy, College of Dentistry, Oral Biology Research Institute, Chosun University) ;
  • Kim, Chong-Gwan (Research Institute for Periodontal regeneration, College of Dentistry, Yonsei University) ;
  • Kim, Byung-Ock (Department of Periodontology, College of Dentistry, Oral Biology Research Institute, Chosun University)
  • 최희준 (조선대학교 치과대학 치주과학교실) ;
  • 지숙 (조선대학교 치과대학 치주과학교실) ;
  • 국중기 (조선대학교 치과대학 구강생물학교실, 구강생물학연구소) ;
  • 장현선 (조선대학교 치과대학 치주과학교실, 구강생물학연구소) ;
  • 박주철 (조선대학교 치과대학 구강조직학교실, 구강생물학연구소) ;
  • 김흥중 (조선대학교 치과대학 구강해부학교실, 구강생물학연구소) ;
  • 김종관 (연세대학교 치과대학 치주조직재생연구소) ;
  • 김병옥 (조선대학교 치과대학 치주과학교실, 구강생물학연구소)
  • Published : 2006.06.30
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Abstract

사람치주인대섬유모세포(human periodontal ligament fibroblast, PDLF)의 기능 손상과 클로르헥시딘(Chlorhexidine, CHX)의 세포독성에 관한 분자적인 기전은 최근까지도 불명확하다. 이 연구의 목적은 PDLF에 의한 골결절 형성에 있어서 CHX의 효과를 평가하고, 치주수술후에 치주병원균의 최소억제농도(minimal inhibitory concentration, MIC)를 평가하고자 하였다. CHX의 세포독성을 평가하기 위해서 MTT assay법을 실시하였다. CHX은 0.12%에서 0.00012%까지, 즉 10-1000배로 희석시킨 후 30, 60, 120초 동안 PDLF에 적용되었고, 석회화된 결절은 alizarin red 용엑에 염색되었다. 치주병원균에 대한 CHX의 MIC가 평가되었다. 이 연구 결과, 세포생존율 검사에서는, 단지 0.12% CHX 에 노출되었던 세포들만 세포 증식 소견을 다소 나타내었다. 모든 CHX 농도(0.12%-0.00012%)에서 PDLF에 의한 골결절 형성은 의미있는 감소를 나타내었다. 또한 치주병원균에 대한 CHX의 MIC는 0.0012%로 나타났다. PDLF의 골결절 형성에 영향을 주는 농도(0.00012%)는 세포독성을 나타내는 농도(0.12%)보다 더 낮은 농도를 보였고, 치주병원균의 최소억제에 필요한 농도는 0.0012%로 나타났다. 이런한 결과들은 통상적으로 상용되는CHX이 PDLF에 의한 골결절 형성에 있어서 영향을 미칠 수 있음을 시사하였다.

Keywords

References

  1. De Sanctis M, Zucchelli G, Clauser C. Bacterial colonization of barrier material and periodontal regeneration. J Clin Periodontol 1996;23:1039-1046 https://doi.org/10.1111/j.1600-051X.1996.tb00534.x
  2. Lindhe J, Karring T, Lang NP. Clinical periodontology and implant dentistry. Munksgaard, Copenhagen, Denmark. 3rd ed. 2000;461-487
  3. Selvig KA, Kersten BG, Chamberlain DH, Wikesjo UME, Nilveus RE. Regenerative surgery of infrabony periodontal defects using ePTFE barrier membrane: Scanning electron microscopic evaluation of retrieved membranes versus clinical healing. J Periodontol 1992;63:974-978 https://doi.org/10.1902/jop.1992.63.12.974
  4. De Sanctis M, Zucchelli G, Clauser C. Bacterial colonization of bioresorbable barrier material and periodontal regeneration. J Periodontol 1996;67:1193-1200 https://doi.org/10.1902/jop.1996.67.11.1193
  5. Zucchelli G, Cesari C, Clauser C, DeSancis M. Early bacterial colonization of guided tissue regeneration membrane materials. An in vivo study. J Periodontol 1998;69:1193-1202 https://doi.org/10.1902/jop.1998.69.11.1193
  6. Alleyn CD, O'Neal RB, Strong SL, et al. The Effect of Chlorhexidine Treatment of root surfaces on the attachment of human gingival fibroblasts in vitro. J Periodontol 1991;62:434-438 https://doi.org/10.1902/jop.1991.62.7.434
  7. Arceo N, Sauk JJ, Moehring J, Foster RA, Somerman MJ. Human periodontal cells initiate mineral-like nodules in vitro. J Periodontol 1991;62:499-503 https://doi.org/10.1902/jop.1991.62.8.499
  8. Nohutcu RM, McCauley LK, Shigeyama Y, Somerman MJ. Expression of mineral-associated proteins by periodontal ligament cells: in vitro vs. ex vivo. J Periodont Res 1996;31:369-372 https://doi.org/10.1111/j.1600-0765.1996.tb00505.x
  9. Nohutch RM, McCauley LK, Koh AJ, Somerman MJ. Expression of extracellular matrix proteins in human periodontal ligament cells during mineralization in vitro. J Periodontol 1997;68:320-327 https://doi.org/10.1902/jop.1997.68.4.320
  10. Paunio KU, Knuuttila M, Mielityinen H. The effect of CHX gluconate on the formation of experimental granulation tissue. J Periodontol 1978;49(2):92-95 https://doi.org/10.1902/jop.1978.49.2.92
  11. Louis SM, Pearson RM. A comparison of the effects of nonoxynol-9 and chlorhexidine on sperm motility. Contraception 1985;32:199-205 https://doi.org/10.1016/0010-7824(85)90108-8
  12. Gabler WL, Roberts D, Harold W. The Effec of Chlorhexidine on blood cells. J. Periodontal Research 1987;22:150-155 https://doi.org/10.1111/j.1600-0765.1987.tb01555.x
  13. Knuuttila M, Soederling E. Effect of chlorhexidine on the release of lysosomal enzymes from cultured macrophases. Acta Odontol Scand 1981;39:285-289 https://doi.org/10.3109/00016358109162291
  14. Hegeland K, Heyden G, Rolla G. Effect of chlorhexidine on animal cells in vitro. Scand J Dent Res 1971;79:209-215
  15. Pucher JJ, Daniel JC. The Effects of Chlorhexidine digluconate on human Fibroblasts in vitro. J. Periodontol 1993;62:526-532
  16. Goldschmidt P, Cogen R, Taubman S. Cytopathologic effects of CHX on human cells. J. Periodontol 1977;48(4):212-215 https://doi.org/10.1902/jop.1977.48.4.212
  17. Cline NV, Layman DL. The Effects of Chlorhexidine on the attachment and growth of cultured periodontal cells. J Periodontol 1992;63:598-602 https://doi.org/10.1902/jop.1992.63.7.598
  18. Chang YC, Huang FM, Tae KW, Chou MY. The effect of sodium hypochlorite and chlorhexidne on cultured human periodontal ligament cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001;92:446-450 https://doi.org/10.1067/moe.2001.116812
  19. Mariotti AJ, Rumpf DA. Chlorhexidine-induced Changes ot human gingival fibroblast collagen and non-collagen protein production. J Periodontol 1999;70:1443-1448 https://doi.org/10.1902/jop.1999.70.12.1443
  20. Bassetti C, Kallenberger A. Influence of Chlorhexidine rinsing on the healing of oral mucosa and osseous lesions. J Clinical Periodontology 1980;7:443-456 https://doi.org/10.1111/j.1600-051X.1980.tb02151.x
  21. Lin SJ, Hou LT, Liu CM, et al. Bacterial morphotypes and early celluar responses in clinically infected and non-infected sites after combination therapy of guided tissue regeneration and allograft. J Dentistry 2000;28:199-206 https://doi.org/10.1016/S0300-5712(99)00067-6
  22. Ling LJ, Hung SL, Lee CF, Chen YT, Wu KM. The influence of membrane exposure on the outcomes of guided tissue regeneration: clinical and microbiological aspects. J Periodont Res 2003;38:57-63 https://doi.org/10.1034/j.1600-0765.2003.01641.x
  23. Urbani G, Graziani A, Lombardo G, Caton JG. Clinical Results with exposed polyglactin 910 resorbable membranes for Guided Tissue Regeneration. Int J Periodont Rest Dent 1997;17:41-51
  24. Hirst RC, Egelberg R, Hornbuckle GC, Oliver RC, Rothbun WE. Microscopic evaluation of topically applied chlorhexidine gluconate on gingival wound healing in dogs. J Southern Calif State Dent Assoc 1973;41:311-317
  25. Hump SE, Rosling B, Lindhe J. Effect of Chlorhexidine on gingival wound healing in the dog: A histometric study. J Clinical Periodontology 1975;2:143-152 https://doi.org/10.1111/j.1600-051X.1975.tb01736.x
  26. Leonard EP, Mandel EJ. Use of Chlorhexidine gluconate to prevent bone resorption in the rice rat. J Dent Res 1979;58(2):672 https://doi.org/10.1177/00220345790580022601
  27. Lindhe J, Heyden G, Svanberg G, Loe H, Schiott C. Effect of local applications of chlorhexidine on the oral mucosa of the hamster. J Periodont Res 1970;5:177-182 https://doi.org/10.1111/j.1600-0765.1970.tb00713.x
  28. Rydbert B, Ahren C. Effect of a cationic detergent on healing of experimenal skin wounds closed by suture and non-suture technique. Acta Chir Scand 1969;135:281-287
  29. Hidalgo E, Dominguez C. Mechanisms underlying Chlorhexidine-induced cytotoxicity. Toxicology in Vitro 2001;15:271-276 https://doi.org/10.1016/S0887-2333(01)00020-0