Porphyromonas gingivalis와 Tannerella forsythia의 응집반응

Coaggregation between Porphyromonas gingivalis and Tannerella forsythia

  • Um, Heung-Sik (Department of Periodontology, College of Dentistry, Kangnung National University) ;
  • Lee, Seok-Woo (Division of Periodontics, School of Dental & Oral Surgery, Columbia University) ;
  • Park, Jae-Hong (Department of Pediatric Dentistry, School of Dentistry, Kyung-Hee University) ;
  • Nauman, R.K. (Department of Microbiology, Baltimore College of Dental Surgery, University of Maryland)
  • 발행 : 2006.03.30

초록

Dental plaque, a biofilm consisting of more than 500 different bacterial species, is an etiological agent of human periodontal disease, It is therefore important to characterize interactions among periodontopathic microorganisms in order to understand the microbial pathogenesis of periodontal disease. Previous data have suggested a synergistic effect of tow major periodontal pathogens Porphyromonas gingivalis and Tannerella forsythia in the periodontal lesion. In the present study, to better understand interaction between P. gingivalis and T. forsythia, the coaggregation activity between these bacteria was characterized. The coaggregation activity was observed by a direct visual assay by mixing equal amount (1 ${\times}$ $10^9$)of T. forsythia and P. gingivaJis cells. It was found that the first aggregates began to appear after 5-10 min, and that the large aggregates completely settled within 1 h. Electron and epifluorescence microscopic studies confirmed cell-cell contact between two bacteria. The heat treatment of P. gingivalis completely blocked the activity, suggesting an involvement of a heat-labile component of P. gingivalis in the interaction. On the other hand, heat treatment of T. forsythia significantly increased the coaggregation activity; the aggregates began to appear immediately. The coaggregation activity was inhibited by addition of protease, however carbohydrates did not inhibit the activity, suggesting that coaggregation is a protein-protein interaction. The results of this study suggest that coaggregation between P. gingivalis and T. forsythia is a result of cell-cell physical contact, and that coaggregation is mediated by a heat-labile component of P. gingivalis and T. forsythia component that can be activated on heat treatment.

키워드

참고문헌

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