Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Activation by Combined Treatment with Cycloheximide and Electrical Stimulation of In-Vitro Matured Porcine Oocytes Improves Subsequent Parthenogenetic Development

  • Naruse Kenji (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Kim Hong-Rye (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Shin Young-Min (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Chang Suk-Min (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Lee Hye-Ran (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Tarte Vaishali (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Quan Yan-Shi (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Kim Beak-Chul (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Park Tae-Young (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Choi Su-Min (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Park Chang-Sik (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Jin Dong-Il (Research Center for Transgenic and Cloned Pigs, Chungnam National University)
  • Published : 2006.03.01
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Abstract

Electrical treatment has been widely used for porcine oocytes activation. However, developmental rates following electrical activation of porcine oocytes is relatively inefficient compared to other domestic animals. To investigate the effects of porcine oocytes on combined activation by both chemical and electrical treatment, in-vitro matured oocytes were activated by combined cycloheximide and electrical pulses treatment. Cumulus-free oocytes were exposed with NCSU-23 medium containing cycloheximide $(10{\mu}g/ml)$ for 0, 5, 10, 20, 30 min and then activated by electrical pulse treatment and cultured in PZM-3 for 8 days. Also effects of exposure to $6.25{\mu}M$ calcium ionophore for 2 min for cumulus-free oocytes were tested. The percentage of blastocyst formation in 10 min exposure to $10{\mu}g/ml$ cycloheximide and electrical pulse treatment was significantly increased (P<0.05) than in the control group. And exposure to $6.25{\mu}M$ calcium ionophore for 2 min with $10{\mu}g/ml$ cycloheximide for 10min and electrical pulse treatment significantly increased (P<0.05) the percentage of blastocyst developmental rates than the control group. In conclusion, activation by combined cycloheximide and electrical stimulation treatment promoted the subsequent development of porcine oocytes and improved the subsequence blastocyst development.

Keywords

References

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