Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Protection of Peroxynitrite-Induced DNA Damage by Dietary Antioxidants

  • Moon Hye-Kyung (School of Life Sciences and Biotechnology and Agro-Biotechnology Education Center, Kyungpook National University) ;
  • Yang Eun-Sun (School of Life Sciences and Biotechnology and Agro-Biotechnology Education Center, Kyungpook National University) ;
  • Park Jeen-Woo (School of Life Sciences and Biotechnology and Agro-Biotechnology Education Center, Kyungpook National University)
  • Published : 2006.03.01
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Abstract

The present study was undertaken to test the hypothesis that dietary antioxidants protect DNA damage induced by peroxynitrite, a potent physiological inorganic toxin. The present study showed that dietary antioxidants such as (-)-epigallocatechin gallate, quercerin, rutin, resveratrol, and ursolic acid inhibit single strand breaks in supercoiled plasmid DNA induced by 3-morpholinosydnomine N-ethylcarbamide (SIN-1), a generator of peroxynitrite through the reaction between nitric oxide and superoxide anion. The formation of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) in calf thymus DNA by SIN-1 was also inhibited by dietary antioxidants. When U937 cells were incubated with 1 mM SIN-1 bolus, a significant increase of 8-OH-dG level was observed. However, oxidative DNA damage was significantly lower in the cells pre-treated with dietary antioxidants when cells were exposed to SIN-1.

Keywords

References

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