The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)
- Volume 19 Issue 3
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- Pages.121-134
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- 2006
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- 1229-4292(pISSN)
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- 2508-3619(eISSN)
Safety and Antioxydative effects of Cuscuta chinensis Lam. in PC12 Cell
PC12 Cell에 대한 토사자(?絲子)의 안정성 및 항산화작용에 대한 연구
- Do, Yong-Ho (Dept. of Ob&Gy, College of Oriental Medicine, Dongguk University) ;
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Kim, Dong-Il
(Dept. of Ob&Gy, College of Oriental Medicine, Dongguk University)
- Published : 2006.08.31
Abstract
Purpose : Cuscuta chinensis Lam. is utilized extensively as important medicines for threatened abortion habitual abortion. However, objective data related to an embryo is not existed until now. Therefore, this study is focused to find out stability of Cuscuta chinensis Lam. about an embryo during pregnancy based on data related to stability of nerve cell and antioxydative effect by using neural cell line, PC 12 cell. Methods : Experimentation concerns cytotoxic effects and antioxydative effect through methods such as MTT ssay, western botting after abstracting an undiluted solution from domestic Cuscuta chinensis Lam. Results : 1. As a result of experimentation on MTT assay according to each magnification from Cuscuta chinensis Lam. extraction solution with different abstraction methods, cytotoxic effect is not observed to all extract except an undiluted solution which is abstracted from MeOH stiring. Also, an undiluted solution in stiring with MeOH could not confirm whether come from Cuscuta chinensis Lam. or not. 2. As a result of revelation of Bax and GSK-3