대한한방부인과학회지 (The Journal of Korean Obstetrics and Gynecology)
- 제19권4호
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- Pages.61-76
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- 2006
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- 1229-4292(pISSN)
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- 2508-3619(eISSN)
안태(安胎)에 활용되는 산약(山藥)의 신경세포주에 대한 안전성 및 항산화효과에 대한 연구
Non-toxic and Anti-oxydative effect of Dioscoreae Rhizoma on PC12 Cell
- 남주영 (동국대학교 한의과대학 부인과학교실) ;
- 노진주 (동국대학교 한의과대학 부인과학교실) ;
- 성준호 (동국대학교 한의과대학 부인과학교실) ;
- 손미영 (동국대학교 이과대학 생물학교실) ;
- 길미정 (동국대학교 이과대학 생물학교실) ;
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성정석
(동국대학교 이과대학 생물학교실) ;
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김동일
(동국대학교 이과대학 생물학교실)
- Nam, Ju-Young (Dept. of OB & GY, College of Oriental Medicine, Dongguk University) ;
- Roh, Jin-Ju (Dept. of OB & GY, College of Oriental Medicine, Dongguk University) ;
- Seung, Jun-Ho (Dept. of OB & GY, College of Oriental Medicine, Dongguk University) ;
- Son, Mi-Young (Dept. of Biology, Collage of Science, Dongguk University) ;
- Khil, Mee-Jeong (Dept. of Biology, Collage of Science, Dongguk University) ;
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Sung, Jung-Suk
(Dept. of Biology, Collage of Science, Dongguk University) ;
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Kim, Dong-Il
(Dept. of Biology, Collage of Science, Dongguk University)
- 발행 : 2006.11.30
초록
Purpose : This study examined the non-toxic and the anti-oxidative effect of Dioscoreae Rhizoma on PC12 cells. Sanyak(Dioscoreae Rhizoma; chinese yam, shan yao) is well-known for its curing power for kidney, lung, spleen. Tonifies and augments the spleen and stomach. Tonifies the lung gi and augments the kidney yin. Tonifies the kidneys and also stabilizes and binds. it also binds the essence and treats spermatorrhea, frequent urination, and vaginal discharge. We are therefore interested in whether Dioscoreae Rhizoma is capable of causing abnormal apoptosis processes, and whether this condition can be rectified through Dioscoreae Rhizoma herb treatment. Methods : We used aqueous extract to treat PC12 cells with different concentrations treated with a water or a MeOH extract of Dioscoreae Rhizoma (0, x10, x20, x40, x80). The MTT (3, (4, 5-dimethyl-thiazol) 2, 5-diphenyl-tetraxolium bromide) reduction assay was employed to quantify the differences in cell activity and viability. The Bax expression level was monitored using western-blotting techniques. The patterns of the changes in expression were scanned and analyzed. Results : Bax and GSK-3