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The Pharmaceutical Society of Korea (대한약학회)
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Cytotoxic and ACAT-inhibitory Sesquiterpene Lactones from the Root of Ixeris dentata forma albiflora

  • Ahn, Eun-Mi (Department of Herbal Food Science, Daegu Haany University) ;
  • Bang, Myun-Ho (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Song, Myoung-Chong (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Park, Mi-Hyun (Erom Life Co. Ltd.) ;
  • Kim, Hwa-Young (Erom Life Co. Ltd.) ;
  • Kwon, Byoung-Mog (Korea Research Institute of Bioscience and Biotechnology) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
  • Published : 2006.11.30
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Abstract

Ixeris dentata forma albiflora was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_{2}O$. Eight sesquiterpenes were isolated through repeated silica gel and octadecyl silica gel ($C_{18},\;ODS$) column chromatography of the EtOAc and n-BuOH fractions. Physicochemical analysis using NMR, MS and IR revealed the chemical structures of the sesquiterpenes, which were zaluzanin (1), 9a-hydroxyguaian-4(15), 10(14), 11 (13)-triene-6, 12-olide(2), $3{\beta}-O-{\beta}-D-glucopyranosyl-8{\beta}-hydroxyguaian$-4(15), 10(14)-diene-6, 12-olide (3), $3-O-{\beta}-D-glucopyranosyl-8{\beta}-hydroxyguauan$-10(14)-ene-6, 12-olide (4), ixerin M (5), glucozaluzanin C (6), crepiside I (7), and ixerin D (8). This is the first time that these sesquiterpene lactones have been isolated from this plant. Compounds 1, 2 and 7 revealed relatively high cytotoxicities on human colon carcinoma cell and lung adeno-carcinoma cell, while compounds 5 and 7 showed acyl-CoA: cholesterol acyltransferase (ACAT) inhibitory activity.

Keywords

References

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